ELISA Kit for Mouse Tumor necrosis factor alpha-induced protein 3

Mfr.No.

E9843m

Alternative Names

Putative DNA-binding protein A20 ; Zinc finger protein A20 ; Tnfip3

Categories

Elisa

Function

Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.

Specificity

Natural and recombinant Mouse Tumor necrosis factor alpha-induced protein 3

Subcellular Location

Cytoplasm Nucleus

Detection Range

0.312-20ng/mL

Entryname

TNAP3_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNAP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-105%	93-102%	95-100%	92-106%
Saliva(n=3)	92-106%	91-108%	90-107%	90-110%
heparin plasma(n=5)	93-104%	94-102%	96-103%	95-101%

Method Type

Sandwich

Platform

PF02338

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤8.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.2%

Protein Name

Tumor necrosis factor alpha-induced protein 3

Recovery

Recovery was determined by spiking various levels of TNAP3 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	98	90-104
Plasma	99	91-107

Sensitivity

0.174ng/mL

Sequence

50:

MAEQLLPQAL ; YLSNMRKAVK ; IRERTPEDIF ; KPTNGIIYHF ; KTMHRYTLEM ;

100:

FRTCQFCPQF ; REIIHKALID ; RSVQASLESQ ; KKLNWCREVR ; KLVALKTNGD ;

150:

GNCLMHAACQ ; YMWGVQDTDL ; VLRKALCSTL ; KETDTRNFKF ; RWQLESLKSQ ;

200:

EFVETGLCYD ; TRNWNDEWDN ; LVKMASADTP ; AARSGLQYNS ; LEEIHIFVLS ;

250:

NILRRPIIVI ; SDKMLRSLES ; GSNFAPLKVG ; GIYLPLHWPA ; QECYRYPIVL ;

300:

GYDSQHFVPL ; VTLKDSGPEL ; RAVPLVNRDR ; GRFEDLKVHF ; LTDPENEMKE ;

350:

KLLKEYLIVM ; EIPVQGWDHG ; TTHLINAAKL ; DEANLPKEIN ; LVDDYFELVQ ;

400:

HEYKKWQENS ; DQARRAAHAQ ; NPLEPSTPQL ; SLMDIKCETP ; NCPFFMSVNT ;

450:

QPLCHECSER ; RQKNQSKLPK ; LNSKLGPEGL ; PGVGLGSSNW ; SPEETAGGPH ;

500:

SAPPTAPSLF ; LFSETTAMKC ; RSPGCPFTLN ; VQHNGFCERC ; HARQINASHT ;

550:

ADPGKCQACL ; QDVTRTFNGI ; CSTCFKRTTA ; EPSSSLTSSI ; PASCHQRSKS ;

600:

DPSQLIQSLT ; PHSCHRTGNV ; SPSGCLSQAA ; RTPGDRAGTS ; KCRKAGCMYF ;

650:

GTPENKGFCT ; LCFIEYRENK ; QSVTASEKAG ; SPAPRFQNNV ; PCLGRECGTL ;

700:

GSTMFEGYCQ ; KCFIEAQNQR ; FHEARRTEEQ ; LRSSQHRDMP ; RTTQVASRLK ;

750:

CARASCKNIL ; ACRSEELCME ; CQHLSQRVGS ; VAHRGEPTPE ; EPPKQRCRAP ;

775:

ACDHFGNAKC ; NGYCNECYQF ; KQMYG

Sequence Length

775

String

Q60769

Sub Unit

Homodimer. Interacts with NAF1, TAX1BP1 and TRAF2. Interacts with RNF11, ITCH and TAX1BP1 only after TNF stimulation; these interaction are transient and they are lost after 1 hour of stimulation with TNF.

Uniprot	Q60769
Gene Name	Tnfaip3
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:21929
Unigene	Mm.116683

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BHE13811620	E9843m	96T	$776		Add to Cart

ELISA Kit for Mouse Tumor necrosis factor alpha-induced protein 3

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