ELISA Kit for Mouse TGF-beta-activated kinase 1 and MAP3K7-binding protein 2

Mfr.No.

E2882m

Alternative Names

Mitogen-activated protein kinase kinase kinase 7-interacting protein 2 ; TAK1-binding protein 2 ; TAB-2 ; TGF-beta-activated kinase 1-binding protein 2 ; Kiaa0733 ; Map3k7ip2

Categories

Elisa

Function

Adapter linking MAP3K7/TAK1 and TRAF6. Promotes MAP3K7 activation in the IL1 signaling pathway. The binding of 'Lys-63'-linked polyubiquitin chains to TAB2 promotes autophosphorylation of MAP3K7 at 'Thr-187' (By similarity). Regulates the IL1-mediated translocation of NCOR1 out of the nucleus. Involved in heart development.

Specificity

Natural and recombinant Mouse TGF-beta-activated kinase 1 and MAP3K7-binding protein 2

Subcellular Location

Nucleus Cytoplasm cytosol Cytoplasmic when activated. Following IL1 stimulation, localized in the to cytosol.

Detection Range

0.156-10ng/mL

Entryname

TAB2_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF02845

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

TGF-beta-activated kinase 1 and MAP3K7-binding protein 2

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sensitivity

0.087ng/mL

Sequence

50:

MAQGSHQIDF ; QVLHDLRQKF ; PEVPEVVVSR ; CMLQNNNNLD ; ACCAVLSQES ;

100:

TRYLYGEGDL ; NFSDESGISG ; LRNHMTSLNL ; DLQSQNVYHH ; GREGSRVNGS ;

150:

RTLTHSVSDG ; QLHGGQSNNE ; LFQQEPQTAP ; AQVPQGFNVF ; GMPSTSGASN ;

200:

STPHLGFHLG ; SKGTSNLSQQ ; TPRFNPIMVT ; LAPNIQTGRS ; TPTSLHIHGV ;

250:

PPPVLNSPQG ; NSIYIRPYIT ; TPSGTARQTQ ; QHSGWVSQFN ; PMNPQQAYQP ;

300:

SQPGPWTTYP ; ASNPLPHTST ; QQPNQQGHQT ; SHVYMPISSP ; TTPQPPTIHS ;

350:

SGSSQSSAHS ; QYNIQNISTG ; PRKNQIEIKL ; EPPQRNSSSK ; LRSSGPRTAS ;

400:

TSSLVNSQTL ; NRNQPTVYIA ; ASPPNTDEMI ; SRSQPKVYIS ; ANATAGDEQG ;

450:

MRNQPTLFIS ; TNSGPSAASR ; NMSGQVSMGP ; AFIHHHPPKS ; RVLGGNSATS ;

500:

PRVVVTQPNT ; KYTFKITVSP ; NKPPAVSPGV ; VSPTFELTNL ; LNHPDHYVET ;

550:

ENIQHLTDPA ; LAHVDRISEA ; RKLSMGSDDA ; AYTQALLVHQ ; KARMERLQRE ;

600:

LEMQKKKLDK ; LKSEVNEMEN ; NLTRRRLKRS ; NSISQIPSLE ; EMQQLRSCNR ;

650:

QLQIDIDCLT ; KEIDLFQARG ; PHFNPSAIHN ; FYDNIGFVGP ; VPPKPKDQRS ;

693:

TIKAPKTQDA ; EDEEGAQWNC ; TACTFLNHPA ; LIRCEQCEMP ; RHF

Sequence Length

693

String

Q99K90

Sub Unit

Interacts with MAP3K7 and TRAF6. Identified in the TRIKA2 complex composed of MAP3K7, TAB1 and TAB2. Binds 'Lys-63'-linked polyubiquitin chains. Interacts with NCOR1 and HDAC3 to form a ternary complex. Interacts (via C-terminal) with NUMBL (via PTB domain). Interacts (via the C-terminus) with WDR34 (via WD domains). Interacts with RBCK1.

Uniprot	Q99K90
Gene Name	Tab2
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:68652
Unigene	Mm.193041

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SKU	Supplier No.	Size	Your price	Quantity
BHE13810697	E2882m	96T	$776		Add to Cart

ELISA Kit for Mouse TGF-beta-activated kinase 1 and MAP3K7-binding protein 2

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