JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Assay Kits / ELISA Kits

ELISA Kit for Mouse Signal transducer and activator of transcription 1

SKU : BHE13809947

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

EIAAB SCIENCE INC, WUHAN

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	E1740m
Categories	Elisa
Function	Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine-and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
Specificity	Natural and recombinant Mouse Signal transducer and activator of transcription 1
Subcellular Location	Cytoplasm Nucleus Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to IFN-gamma and signaling by activated FGFR1; FGFR2; FGFR3 or FGFR4.
Detection Range	0.156-10ng/mL
Entryname	STAT1_MOUSE
Method Type	Sandwich
Platform	PF00017
Protein Name	Signal transducer and activator of transcription 1
Sensitivity	0.078ng/mL
Sequence	50: MSQWFELQQL ; DSKFLEQVHQ ; LYDDSFPMEI ; RQYLAQWLEK ; QDWEHAAYDV ; 100: SFATIRFHDL ; LSQLDDQYSR ; FSLENNFLLQ ; HNIRKSKRNL ; QDNFQEDPVQ ; 150: MSMIIYNCLK ; EERKILENAQ ; RFNQAQEGNI ; QNTVMLDKQK ; ELDSKVRNVK ; 200: DQVMCIEQEI ; KTLEELQDEY ; DFKCKTSQNR ; EGEANGVAKS ; DQKQEQLLLH ; 250: KMFLMLDNKR ; KEIIHKIREL ; LNSIELTQNT ; LINDELVEWK ; RRQQSACIGG ; 300: PPNACLDQLQ ; TWFTIVAETL ; QQIRQQLKKL ; EELEQKFTYE ; PDPITKNKQV ; 350: LSDRTFLLFQ ; QLIQSSFVVE ; RQPCMPTHPQ ; RPLVLKTGVQ ; FTVKSRLLVK ; 400: LQESNLLTKV ; KCHFDKDVNE ; KNTVKGFRKF ; NILGTHTKVM ; NMEESTNGSL ; 450: AAELRHLQLK ; EQKNAGNRTN ; EGPLIVTEEL ; HSLSFETQLC ; QPGLVIDLET ; 500: TSLPVVVISN ; VSQLPSGWAS ; ILWYNMLVTE ; PRNLSFFLNP ; PCAWWSQLSE ; 550: VLSWQFSSVT ; KRGLNADQLS ; MLGEKLLGPN ; AGPDGLIPWT ; RFCKENINDK ; 600: NFSFWPWIDT ; ILELIKNDLL ; CLWNDGCIMG ; FISKERERAL ; LKDQQPGTFL ; 650: LRFSESSREG ; AITFTWVERS ; QNGGEPDFHA ; VEPYTKKELS ; AVTFPDIIRN ; 700: YKVMAAENIP ; ENPLKYLYPN ; IDKDHAFGKY ; YSRPKEAPEP ; MELDDPKRTG ; 749: YIKTELISVS ; EVHPSRLQTT ; DNLLPMSPEE ; FDEMSRIVGP ; EFDSMMSTV
Sequence Length	749
String	P42225
Sub Unit	In response to IFN alpha/beta, three subunits (STAT1-alpha, STAT1-beta, STAT2) of ISGF3, become phosphorylated on tyrosine, migrate into the nucleus, and assemble into a complex together with ISGF3 gamma (p48), a DNA-binding protein that specifically binds to the IFN-stimulated response element. In response to IFN gamma, STAT1 forms homodimers, that also translocate into the nucleus to activate IFN gamma-responsive genes. Interacts with PIAS1 (dimethylated on arginine); the interaction results in release of STAT1 from its target gene (By similarity). Interacts with NMI. Interacts with PIAS1; the interaction requires phosphorylation on Ser-727 and inhibits STAT1 activation. Interacts with IFNAR1 and IFNAR2.

Uniprot	P42225
Gene Name	Stat1
Immunogen Species	Mus musculus (Mouse)
Unigene	Mm.277406

Reference	1. "Stat3 and Stat4: members of the family of signal transducers and activators of transcription." Zhong Z., Wen Z., Darnell J.E. Jr. Proc. Natl. Acad. Sci. U.S.A.91:4806-4810(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Thymus. 2. "c-Src activates both STAT1 and STAT3 in PDGF-stimulated NIH3T3 cells." Cirri P., Chiarugi P., Marra F., Raugei G., Camici G., Manao G., Ramponi G. Biochem. Biophys. Res. Commun.239:493-497(1997) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION;FUNCTION;INTERACTION WITH SRC 3. "Identification of tyrosine residues in constitutively activated fibroblast growth factor receptor 3 involved in mitogenesis, Stat activation, and phosphatidylinositol 3-kinase activation." Hart K.C., Robertson S.C., Donoghue D.J. Mol. Biol. Cell12:931-942(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;PHOSPHORYLATION 4. "Identification of a nuclear Stat1 protein tyrosine phosphatase." ten Hoeve J., de Jesus Ibarra-Sanchez M., Fu Y., Zhu W., Tremblay M., David M., Shuai K. Mol. Cell. Biol.22:5662-5668(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION;DEPHOSPHORYLATION BY PTPN2 5. "Proteomic identification of proteins conjugated to ISG15 in mouse and human cells." Giannakopoulos N.V., Luo J.K., Papov V., Zou W., Lenschow D.J., Jacobs B.S., Borden E.C., Li J., Virgin H.W., Zhang D.E. Biochem. Biophys. Res. Commun.336:496-506(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ISGYLATION 6. "Multiple functions of the IKK-related kinase IKKepsilon in interferon-mediated antiviral immunity." Tenoever B.R., Ng S.L., Chua M.A., McWhirter S.M., Garcia-Sastre A., Maniatis T. Science315:1274-1278(2007) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN THE ISGF3 COMPLEX;PHOSPHORYLATION AT SER-708 AND SER-744;MUTAGENESIS OF SER-708 AND 744-SER--SER-747 7. "Analysis of STAT1 activation by six FGFR3 mutants associated with skeletal dysplasia undermines dominant role of STAT1 in FGFR3 signaling in cartilage." Krejci P., Salazar L., Kashiwada T.A., Chlebova K., Salasova A., Thompson L.M., Bryja V., Kozubik A., Wilcox W.R. PLoS ONE3:E3961-E3961(2008) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;PHOSPHORYLATION AT TYR-701 IN RESPONSE TO CONSTITUTIVELY ACTIVATED FGFR3 8. "Mechanisms of STAT protein activation by oncogenic KIT mutants in neoplastic mast cells." Chaix A., Lopez S., Voisset E., Gros L., Dubreuil P., De Sepulveda P. J. Biol. Chem.286:5956-5966(2011) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION IN RESPONSE TO KIT SIGNALING 9. "Inhibitor of kappaB kinase epsilon (IKK(epsilon)), STAT1, and IFIT2 proteins define novel innate immune effector pathway against West Nile virus infection." Perwitasari O., Cho H., Diamond M.S., Gale M. Jr. J. Biol. Chem.286:44412-44423(2011) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;PHOSPHORYLATION AT TYR-701; SER-708 AND SER-727

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13809947	E1740m	96T	$776		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.