ELISA Kit for Mouse S-phase kinase-associated protein 2

Mfr.No.

E9870m

Alternative Names

Cyclin-A/CDK2-associated protein p45 ; F-box protein Skp2 ; F-box/WD-40 protein 1 ; FWD1

Categories

Elisa

Function

Substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. The SCF complex provides substrate specificity and interacts with both, the E2 ubiquitin-conjugating enzyme and the substrate. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1.

Specificity

Natural and recombinant Mouse S-phase kinase-associated protein 2

Detection Range

0.312-20ng/mL

Entryname

SKP2_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF00646

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.6%

Protein Name

S-phase kinase-associated protein 2

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	92-103
Plasma	103	95-107

Sensitivity

0.175ng/mL

Sequence

50:

MHRKHLQEIP ; DQSGNVTTSF ; TWGWDSSKTS ; ELLSGMGVSA ; LEKEEVDSEN ;

100:

IPHGLLSNLG ; HPQSPPRKRV ; KGKGSDKDFV ; IIRRPKLSRE ; NFPGVSWDSL ;

150:

PDELLLGIFS ; CLCLPELLRV ; SGVCKRWYRL ; SLDESLWQSL ; DLAGKNLHPD ;

200:

VTVRLLSRGV ; VAFRCPRSFM ; EQPLGESFSS ; FRVQHMDLSN ; SVINVSNLHK ;

250:

ILSECSKLQN ; LSLEGLQLSD ; PIVKTLAQNE ; NLVRLNLCGC ; SGFSESAVAT ;

300:

LLSSCSRLDE ; LNLSWCFDFT ; EKHVQAAVAH ; LPNTITQLNL ; SGYRKNLQKT ;

350:

DLCTIIKRCP ; NLIRLDLSDS ; IMLKNDCFPE ; FFQLNYLQHL ; SLSRCYDIIP ;

400:

DTLLELGEIP ; TLKTLQVFGI ; VPEGTLQLLR ; EALPRLQINC ; AYFTTIARPT ;

424:

MDSKKNLEIW ; GIKCRLTLQK ; PSCL

Sequence Length

424

String

Q9Z0Z3

Sub Unit

Part of a SCF(SKP2) complex consisting of CUL1, RBX1, SKP1 and SKP2. Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Interacts directly with CUL1 and SKP1. Interacts with CKS1. Interacts with the cyclin-A-CDK2 complex. Interacts with ORC1, phosphorylated CDT1, phosphorylated RBL2, ELF4, phosphorylated RAG2, FOXO1A, UBP43, MYC, TOB1, TAL1 and MLL. Interacts with TRIM21.

Uniprot	Q9Z0Z3
Gene Name	Skp2
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:27401
Unigene	Mm.35584

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SKU	Supplier No.	Size	Your price	Quantity
BHE13810295	E9870m	96T	$776		Add to Cart

ELISA Kit for Mouse S-phase kinase-associated protein 2

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