ELISA Kit for Mouse Protein kinase C gamma type

Mfr.No.

E8823m

Alternative Names

Pkcc ; Pkcg ; Prkcc

Categories

Elisa

Function

Calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. Serves as the receptor for phorbol esters, a class of tumor promoters. Phosphorylates a number of proteins involved in neuronal development and is required for stress responses in neurological tissue. Modulates cerebellar neural transmission. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum.

Specificity

Natural and recombinant Mouse Protein kinase C gamma type

Subcellular Location

Cytoplasm Cell membrane Peripheral membrane protein Cell junction synapse synaptosome Translocates to synaptic membranes on stimulation.

Detection Range

0.156-10ng/mL

Entryname

KPCG_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PKCG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	94-103%	93-106%	96-102%	91-107%
EDTA plasma(n=5)	92-104%	90-108%	92-104%	94-104%
heparin plasma(n=5)	95-103%	91-106%	93-102%	96-100%

Method Type

Sandwich

Platform

PF00130

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.8%

Protein Name

Protein kinase C gamma type

Recovery

Recovery was determined by spiking various levels of PKCG into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	93-105
Plasma	98	94-106

Sensitivity

0.087ng/mL

Sequence

50:

MAGLGPGGGD ; SEGGPRPLFC ; RKGALRQKVV ; HEVKSHKFTA ; RFFKQPTFCS ;

100:

HCTDFIWGIG ; KQGLQCQVCS ; FVVHRRCHEF ; VTFECPGAGK ; GPQTDDPRNK ;

150:

HKFRLHSYSS ; PTFCDHCGSL ; LYGLVHQGMK ; CSCCEMNVHR ; RCVRSVPSLC ;

200:

GVDHTERRGR ; LQLEIRAPTS ; DEIHITVGEA ; RNLIPMDPNG ; LSDPYVKLKL ;

250:

IPDPRNLTKQ ; KTKTVKATLN ; PVWNETFVFN ; LKPGDVERRL ; SVEVWDWDRT ;

300:

SRNDFMGAMS ; FGVSELLKAP ; VDGWYKLLNQ ; EEGEYYNVPV ; ADADNCSLLQ ;

350:

KFEACNYPLE ; LYERVRMGPS ; SSPIPSPSPS ; PTDSKRCFFG ; ASPGRLHISD ;

400:

FSFLMVLGKG ; SFGKVMLAER ; RGSDELYAIK ; ILKKDVIVQD ; DDVDCTLVEK ;

450:

RVLALGGRGP ; GGRPHFLTQL ; HSTFQTPDRL ; YFVMEYVTGG ; DLMYHIQQLG ;

500:

KFKEPHAAFY ; AAEIAIGLFF ; LHNQGIIYRD ; LKLDNVMLDA ; EGHIKITDFG ;

550:

MCKENVFPGS ; TTRTFCGTPD ; YIAPEIIAYQ ; PYGKSVDWWS ; FGVLLYEMLA ;

600:

GQPPFDGEDE ; EELFQAIMEQ ; TVTYPKSLSR ; EAVAICKGFL ; TKHPGKRLGS ;

650:

GPDGEPTIRA ; HGFFRWIDWE ; RLERLEIAPP ; FRPRPCGRSG ; ENFDKFFTRA ;

697:

APALTPPDRL ; VLASIDQADF ; QGFTYVNPDF ; VHPDARSPTS ; PVPVPVM

Sequence Length

697

String

P63318

Sub Unit

Interacts with CDCP1.

Uniprot	P63318
Gene Name	Prkcg
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:18752
Unigene	Mm.7980

Reference	1. "Isolation and sequence of a mouse brain cDNA coding for protein kinase C-gamma isozyme." Bowers B.J., Parham C.L., Sikela J.M., Wehner J.M. Gene123:263-265(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Brain. 2. Tseng C.P., Verma A. Submitted (1994-01) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: BALB/c. tissue: Brain. 3. "Modified hippocampal long-term potentiation in PKC gamma-mutant mice." Abeliovich A., Chen C., Goda Y., Silva A.J., Stevens C.F., Tonegawa S. Cell75:1253-1262(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN LONG-TERM POTENTIATION 4. "Impaired motor coordination correlates with persistent multiple climbing fiber innervation in PKC gamma mutant mice." Chen C., Kano M., Abeliovich A., Chen L., Bao S., Kim J.J., Hashimoto K., Thompson R.F., Tonegawa S. Cell83:1233-1242(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE 5. "Preserved acute pain and reduced neuropathic pain in mice lacking PKCgamma." Malmberg A.B., Chen C., Tonegawa S., Basbaum A.I. Science278:279-283(1997) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN NEUROPATHIC PAIN 6. "Enhanced mu-opioid responses in the spinal cord of mice lacking protein kinase Cgamma isoform." Narita M., Mizoguchi H., Suzuki T., Narita M., Dun N.J., Imai S., Yajima Y., Nagase H., Suzuki T., Tseng L.F. J. Biol. Chem.276:15409-15414(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN OPIOID SIGNALING 7. "Involvement of spinal protein kinase Cgamma in the attenuation of opioid mu-receptor-mediated G-protein activation after chronic intrathecal administration of [D-Ala2,N-MePhe4,Gly-Ol(5)]enkephalin." Narita M., Mizoguchi H., Narita M., Nagase H., Suzuki T., Tseng L.F. J. Neurosci.21:3715-3720(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN OPIOID SIGNALING 8. "Involvement of protein kinase Cgamma isoform in morphine-induced reinforcing effects." Narita M., Aoki T., Ozaki S., Yajima Y., Suzuki T. Neuroscience103:309-314(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN OPIOID SIGNALING 9. "Reduced development of tolerance to the analgesic effects of morphine and clonidine in PKC gamma mutant mice." Zeitz K.P., Malmberg A.B., Gilbert H., Basbaum A.I. Pain94:245-253(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN OPIOID SIGNALING 10. "Abnormal features in mutant cerebellar Purkinje cells lacking junctophilins." Ikeda A., Miyazaki T., Kakizawa S., Okuno Y., Tsuchiya S., Myomoto A., Saito S.Y., Yamamoto T., Yamazaki T., Iino M., Tsujimoto G., Watanabe M., Takeshima H. Biochem. Biophys. Res. Commun.363:835-839(2007) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-514; THR-655 AND THR-674;SUBCELLULAR LOCATION;FUNCTION;TISSUE SPECIFICITY 11. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics6:283-293(2007) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS] tissue: Brain cortex. 12. "Isoflurane inhibits protein kinase Cgamma and calcium/calmodulin dependent protein kinase ii-alpha translocation to synaptic membranes in ischemic mice brains." Matsumoto S., Murozono M., Nagaoka D., Matsuoka S., Takeda A., Narita H., Watanabe S., Isshiki A., Watanabe Y. Neurochem. Res.33:2302-2309(2008) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION 13. "PKCgamma participates in food entrainment by regulating BMAL1." Zhang L., Abraham D., Lin S.T., Oster H., Eichele G., Fu Y.H., Ptacek L.J. Proc. Natl. Acad. Sci. U.S.A.109:20679-20684(2012) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH ARNTL;DISRUPTION PHENOTYPE;TISSUE SPECIFICITY;PHOSPHORYLATION AT THR-655

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ELISA Kit for Mouse Protein kinase C gamma type

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