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ELISA Kit for Mouse Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

SKU : BHE13808685

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E14307m
Alternative Names	Phosphatidylinositol 4 ; 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha ; PtdIns-3-kinase subunit p110-alpha ; Phosphoinositide-3-kinase catalytic alpha polypeptide ; Serine/threonine protein kinase PIK3CA ; 2.7.11.1
Categories	Elisa
Function	Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol-3,4,5-triphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS.
Specificity	Natural and recombinant Mouse Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Detection Range	0.78-50ng/mL
Entryname	PK3CA_MOUSE
Method Type	Sandwich
Platform	PF00454
Protein Name	Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Sensitivity	0.39ng/mL
Sequence	50: MPPRPSSGEL ; WGIHLMPPRI ; LVECLLPNGM ; IVTLECLREA ; TLVTIKHELF ; 100: REARKYPLHQ ; LLQDETSYIF ; VSVTQEAERE ; EFFDETRRLC ; DLRLFQPFLK ; 150: VIEPVGNREE ; KILNREIGFV ; IGMPVCEFDM ; VKDPEVQDFR ; RNILNVCKEA ; 200: VDLRDLNSPH ; SRAMYVYPPN ; VESSPELPKH ; IYNKLDKGQI ; IVVIWVIVSP ; 250: NNDKQKYTLK ; INHDCVPEQV ; IAEAIRKKTR ; SMLLSSEQLK ; LCVLEYQGKY ; 300: ILKVCGCDEY ; FLEKYPLSQY ; KYIRSCIMLG ; RMPNLMLMAK ; ESLYSQLPID ; 350: SFTMPSYSRR ; ISTATPYMNG ; ETSTKSLWVI ; NSALRIKILC ; ATYVNVNIRD ; 400: IDKIYVRTGI ; YHGGEPLCDN ; VNTQRVPCSN ; PRWNEWLNYD ; IYIPDLPRAA ; 450: RLCLSICSVK ; GRKGAKEEHC ; PLAWGNINLF ; DYTDTLVSGK ; MALNLWPVPH ; 500: GLEDLLNPIG ; VTGSNPNKET ; PCLELEFDWF ; SSVVKFPDMS ; VIEEHANWSV ; 550: SREAGFSYSH ; TGLSNRLARD ; NELRENDKEQ ; LRALCTRDPL ; SEITEQEKDF ; 600: LWSHRHYCVT ; IPEILPKLLL ; SVKWNSRDEV ; AQMYCLVKDW ; PPIKPEQAME ; 650: LLDCNYPDPM ; VRSFAVRCLE ; KYLTDDKLSQ ; YLIQLVQVLK ; YEQYLDNLLV ; 700: RFLLKKALTN ; QRIGHFFFWH ; LKSEMHNKTV ; SQRFGLLLES ; YCRACGMYLK ; 750: HLNRQVEAME ; KLINLTDILK ; QEKKDETQKV ; QMKFLVEQMR ; QPDFMDALQG ; 800: FLSPLNPAHQ ; LGNLRLEECR ; IMSSAKRPLW ; LNWENPDIMS ; ELLFQNNEII ; 850: FKNGDDLRQD ; MLTLQIIRIM ; ENIWQNQGLD ; LRMLPYGCLS ; IGDCVGLIEV ; 900: VRNSHTIMQI ; QCKGGLKGAL ; QFNSHTLHQW ; LKDKNKGEIY ; DAAIDLFTRS ; 950: CAGYCVATFI ; LGIGDRHNSN ; IMVKDDGQLF ; HIDFGHFLDH ; KKKKFGYKRE ; 1000: RVPFVLTQDF ; LIVISKGAQE ; YTKTREFERF ; QEMCYKAYLA ; IRQHANLFIN ; 1050: LFSMMLGSGM ; PELQSFDDIA ; YIRKTLALDK ; TEQEALEYFT ; KQMNDAHHGG ; 1068: WTTKMDWIFH ; TIKQHALN
Sequence Length	1068
String	P42337
Sub Unit	Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3) (By similarity). Interacts with IRS1 in nuclear extracts (By similarity). Interacts with RUFY3 (By similarity). Interacts with RASD2 (By similarity). Interacts with APPL1 (By similarity). Interacts with HRAS1 and KRAS (By similarity). Interaction with HRAS1/KRAS is required for PI3K pathway signaling and cell proliferation stimulated by EGF and FGF2.

Uniprot	P42337
Gene Name	Pik3ca
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:18706
Unigene	Mm.260521

Reference	1. "The interaction of small domains between the subunits of phosphatidylinositol 3-kinase determines enzyme activity." Klippel A., Escobedo J.A., Hirano M., Williams L.T. Mol. Cell. Biol.14:2675-2685(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: BALB/c. 2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (2005-07) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] 3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: C57BL/6. tissue: Eye. 4. "Control of cell size through phosphorylation of upstream binding factor 1 by nuclear phosphatidylinositol 3-kinase." Drakas R., Tu X., Baserga R. Proc. Natl. Acad. Sci. U.S.A.101:9272-9276(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH IRS1 5. "A pharmacological map of the PI3-K family defines a role for p110alpha in insulin signaling." Knight Z.A., Gonzalez B., Feldman M.E., Zunder E.R., Goldenberg D.D., Williams O., Loewith R., Stokoe D., Balla A., Toth B., Balla T., Weiss W.A., Williams R.L., Shokat K.M. Cell125:733-747(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION 6. "Critical role for the p110alpha phosphoinositide-3-OH kinase in growth and metabolic regulation." Foukas L.C., Claret M., Pearce W., Okkenhaug K., Meek S., Peskett E., Sancho S., Smith A.J.H., Withers D.J., Vanhaesebroeck B. Nature441:366-370(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DISRUPTION PHENOTYPE;MUTAGENESIS OF ASP-933 7. "The p110alpha isoform of PI3K is essential for proper growth factor signaling and oncogenic transformation." Zhao J.J., Cheng H., Jia S., Wang L., Gjoerup O.V., Mikami A., Roberts T.M. Proc. Natl. Acad. Sci. U.S.A.103:16296-16300(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DISRUPTION PHENOTYPE 8. "Binding of ras to phosphoinositide 3-kinase p110alpha is required for ras-driven tumorigenesis in mice." Gupta S., Ramjaun A.R., Haiko P., Wang Y., Warne P.H., Nicke B., Nye E., Stamp G., Alitalo K., Downward J. Cell129:957-968(2007) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DISRUPTION PHENOTYPE;INTERACTION WITH HRAS AND KRAS;MUTAGENESIS OF THR-208 AND LYS-227 9. "Angiogenesis selectively requires the p110alpha isoform of PI3K to control endothelial cell migration." Graupera M., Guillermet-Guibert J., Foukas L.C., Phng L.-K., Cain R.J., Salpekar A., Pearce W., Meek S., Millan J., Cutillas P.R., Smith A.J.H., Ridley A.J., Ruhrberg C., Gerhardt H., Vanhaesebroeck B. Nature453:662-666(2008) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DISRUPTION PHENOTYPE 10. "VEGF-mediated PI3K class IA and PKC signaling in cardiomyogenesis and vasculogenesis of mouse embryonic stem cells." Bekhite M.M., Finkensieper A., Binas S., Mueller J., Wetzker R., Figulla H.-R., Sauer H., Wartenberg M. J. Cell Sci.124:1819-1830(2011) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION ON CARDIOMYOGENESIS;FUNCTION ON VASCULOGENESIS

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