ELISA Kit for Mouse Occludin

Mfr.No.

E14928m

Alternative Names

Ocl

Categories

Elisa

Function

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Specificity

Natural and recombinant Mouse Occludin

Subcellular Location

Membrane Multi-pass membrane protein Cell junction tight junction

Detection Range

0.156-10ng/mL

Entryname

OCLN_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OCLN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-102%	90-103%	93-98%	91-100%
EDTA plasma(n=5)	90-106%	93-101%	91-102%	93-102%
heparin plasma(n=5)	93-103%	94-100%	92-100%	91-104%

Method Type

Sandwich

Platform

PF01284

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.7%

Protein Name

Occludin

Recovery

Recovery was determined by spiking various levels of OCLN into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	91-103
Plasma	97	93-106

Sensitivity

0.078ng/mL

Sequence

50:

MSVRPFESPP ; PYRPDEFKPN ; HYAPSNDMYG ; GEMHVRPMLS ; QPAYSFYPED ;

100:

EILHFYKWTS ; PPGVIRILSM ; LIIVMCIAIF ; ACVASTLAWD ; RGYGTGLFGG ;

150:

SLNYPYSGFG ; YGGGYGGGYG ; GYGYGYGGYT ; DPRAAKGFLL ; AMAAFCFIAS ;

200:

LVIFVTSVIR ; SGMSRTRRYY ; LIVIIVSAIL ; GIMVFIATIV ; YIMGVNPTAQ ;

250:

ASGSMYGSQI ; YMICNQFYTP ; GGTGLYVDQY ; LYHYCVVDPQ ; EAIAIVLGFM ;

300:

IIVAFALIIF ; FAVKTRRKMD ; RYDKSNILWD ; KEHIYDEQPP ; NVEEWVKNVS ;

350:

AGTQDMPPPP ; SDYAERVDSP ; MAYSSNGKVN ; GKRSYPESFY ; KSTPLVPEVA ;

400:

QEIPLTLSVD ; DFRQPRYSSN ; GNLETPSKRA ; PTKGKAGKGK ; RTDPDHYETD ;

450:

YTTGGESCEE ; LEEDWVREYP ; PITSDQQRQL ; YKRNFDAGLQ ; EYKSLQAELD ;

500:

DVNKELSRLD ; KELDDYREES ; EEYMAAADEY ; NRLKQVKGSA ; DYKSKRNYCK ;

521:

QLKSKLSHIK ; RMVGDYDRRK ; P

Sequence Length

521

String

Q61146

Sub Unit

Interacts with TJP1/ZO1 and with VAPA.

Uniprot	Q61146
Gene Name	Ocln
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:18260
Unigene	Mm.4807

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SKU	Supplier No.	Size	Your price	Quantity
BHE13808515	E14928m	96T	$776		Add to Cart

ELISA Kit for Mouse Occludin

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