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Home / Assay Kits / ELISA Kits

ELISA Kit for Mouse NAD-dependent protein deacylase sirtuin-5, mitochondrial

SKU : BHE13810461

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E11384m
Antigen	SIRT5
Alternative Names	Regulatory protein SIR2 homolog 5 ; SIR2-like protein 5 ; Sir2l5
Categories	Elisa
Function	NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as Uox.
Specificity	Natural and recombinant Mouse NAD-dependent protein deacylase sirtuin-5, mitochondrial
Subcellular Location	Mitochondrion Cytoplasm Cytosol Nucleus Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small amounts are also detected in the nucleus.
Detection Range	0.156-10ng/mL
Entryname	SIR5_MOUSE
Method Type	Sandwich
Platform	PF02146
Protein Name	NAD-dependent protein deacylase sirtuin-5, mitochondrial
Sensitivity	0.086ng/mL
Sequence	50: MRPLLIAPGR ; FISQLCCRRK ; PPASPQSKIC ; LTMARPSSNM ; ADFRKCFANA ; 100: KHIAIISGAG ; VSAESGVPTF ; RGAGGYWRKW ; QAQDLATPQA ; FARNPSQVWE ; 150: FYHYRREVMR ; SKEPNPGHLA ; IAQCEARLRD ; QGRRVVVITQ ; NIDELHRKAG ; 200: TKNLLEIHGT ; LFKTRCTSCG ; TVAENYRSPI ; CPALAGKGAP ; EPETQDARIP ; 250: VDKLPRCEEA ; GCGGLLRPHV ; VWFGENLDPA ; ILEEVDRELA ; LCDLCLVVGT ; 300: SSVVYPAAMF ; APQVASRGVP ; VAEFNMETTP ; ATDRFRFHFP ; GPCGKTLPEA ; 310: LAPHETERTS ;
Sequence Length	310
Sub Unit	Monomer. Homodimer. Interacts with CPS1.

Uniprot	Q8K2C6
Gene Name	Sirt5
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:68346
Unigene	Mm.35325

Reference	1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] tissue: Mammary tumor. 2. "SIRT5 Deacetylates carbamoyl phosphate synthetase 1 and regulates the urea cycle." Nakagawa T., Lomb D.J., Haigis M.C., Guarente L. Cell137:560-570(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF N-TERMINUS;FUNCTION;MUTAGENESIS OF HIS-158;SUBCELLULAR LOCATION;INTERACTION WITH CPS1;DISRUPTION PHENOTYPE;TISSUE SPECIFICITY 3. "The first identification of lysine malonylation substrates and its regulatory enzyme." Peng C., Lu Z., Xie Z., Cheng Z., Chen Y., Tan M., Luo H., Zhang Y., He W., Yang K., Zwaans B.M., Tishkoff D., Ho L., Lombard D., He T.C., Dai J., Verdin E., Ye Y., Zhao Y.more... Mol. Cell. Proteomics10:M111.012658.01-M111.012658.12(2011) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DISRUPTION PHENOTYPE 4. "Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase." Du J., Zhou Y., Su X., Yu J.J., Khan S., Jiang H., Kim J., Woo J., Kim J.H., Choi B.H., He B., Chen W., Zhang S., Cerione R.A., Auwerx J., Hao Q., Lin H. Science334:806-809(2011) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DISRUPTION PHENOTYPE 5. "SIRT5 deacetylates and activates urate oxidase in liver mitochondria of mice." Nakamura Y., Ogura M., Ogura K., Tanaka D., Inagaki N. FEBS Lett.586:4076-4081(2012) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION 6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways." Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y. Mol. Cell50:919-930(2013) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;DISRUPTION PHENOTYPE 7. "Metabolic characterization of a Sirt5 deficient mouse model." Yu J., Sadhukhan S., Noriega L.G., Moullan N., He B., Weiss R.S., Lin H., Schoonjans K., Auwerx J. Sci. Rep.3:2806-2806(2013) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE

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SKU	Supplier No.	Size	Your price	Quantity
BHE13810461	E11384m	96T	$776		Add to Cart

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