ELISA Kit for Mouse Leucine-rich PPR motif-containing protein, mitochondrial

Mfr.No.

E14393m

Alternative Names

130 kDa leucine-rich protein ; LRP 130 ; Lrp130

Categories

Elisa

Function

May play a role in RNA metabolism in both nuclei and mitochondria. In the nucleus binds to HNRPA1-associated poly(A) mRNAs and is part of nmRNP complexes at late stages of mRNA maturation which are possibly associated with nuclear mRNA export. May bind mature mRNA in the nucleus outer membrane. In mitochondria binds to poly(A) mRNA. Plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. May be involved in transcription regulation. Cooperates with PPARGC1A to regulate certain mitochondrially encoded genes and gluconeogenic genes and may regulate docking of PPARGC1A to transcription factors. Seems to be involved in the transcription regulation of the multidrug-related genes MDR1 and MVP. Part of a nuclear factor that binds to the invMED1 element of MDR1 and MVP gene promoters (By similarity). Binds single-stranded DNA.

Specificity

Natural and recombinant Mouse Leucine-rich PPR motif-containing protein, mitochondrial

Subcellular Location

Mitochondrion Nucleus Nucleus nucleoplasm Nucleus inner membrane Nucleus outer membrane

Detection Range

78-5000pg/mL

Entryname

LPPRC_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	88-103%	88-101%	91-105%	89-103%
EDTA plasma(n=5)	87-97%	91-103%	92-101%	88-105%
heparin plasma(n=5)	90-104%	94-108%	101-106%	91-109%

Method Type

Sandwich

Platform

PF01535

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 5.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 8.7%

Protein Name

Leucine-rich PPR motif-containing protein, mitochondrial

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	93-102
Plasma	99	95-105

Sensitivity

39.04pg/mL

Sequence

50:

MAALLRPARW ; LLGAAAAPRL ; PLSLRLPAGV ; PGRLSSVVRV ; AAVGSRPAAG ;

100:

ERLSQARLYA ; IVAEKRDLQE ; EPAPVRKNSS ; QFDWALMRLD ; NSVRRTGRIT ;

150:

KGLLQRVFES ; TCSSGSPGSN ; QALLLLRSCG ; SLLPELSLAE ; RTEFAHKIWD ;

200:

KLQQLGVVYD ; VSHYNALLKV ; YLQNEYKFSP ; TDFLAKMEGA ; NIQPNRVTYQ ;

250:

RLIAAYCNVG ; DIEGASKILG ; FMKTKDLPIT ; EAVFSALVTG ; HARAGDMENA ;

300:

ENILTVMKQA ; GIEPGPDTYL ; ALLNAHAERG ; DIGQVRQILE ; KVEKSDHYFM ;

350:

DRDFLQVIFS ; FSKAGYPQYV ; SEILEKITYE ; RRSIPDAMNL ; ILFLATEKLE ;

400:

DTAFQVLLAL ; PLSKDESSDN ; FGSFFLRHCV ; TLDLPPEKLI ; DYCRRLRDAK ;

450:

LHSSSLQFTL ; HCALQANRTA ; LAKAVMEALR ; EEGFPIRPHY ; FWPLLAGHQK ;

500:

TKNVQGIIDI ; LKIMNKVGVD ; PDQETYINYV ; FPCFDSAQSV ; RAALQENECL ;

550:

LASSTFAQAE ; VKNEAINGNL ; QNILSFLESN ; TLPFSFSSLR ; NSLILGFRRS ;

600:

MNIDLWSKIT ; ELLYKDERYC ; SKPPGPAEAV ; GYFLYNLIDS ; MSDSEVQAKE ;

650:

ERLRQYFHQL ; QEMNVKVPEN ; IYKGICNLLN ; TYHVPELIKD ; IKVLVDREKV ;

700:

DSQKTSQVTS ; SDLESTLEKL ; KAEGQPVGSA ; LKQLLLLLCS ; EENMQKALEV ;

750:

KAKYESDMVI ; GGYAALINLC ; CRHDNAEDAW ; NLKQEVDRLD ; ASAILDTAKY ;

800:

VALVKVLGKH ; SRLQDAINIL ; KEMKEKDVVI ; KDATVLSFFH ; ILNGAALRGE ;

850:

IETVKQLHEA ; IVTLGLAKPS ; SNISFPLVTV ; HLEKGDLPAA ; LEASIACHKK ;

900:

YKVLPRIHDV ; LCKLVEKGET ; DLIQKAMDFV ; SQEQGEMTML ; YDLFFAFLQT ;

950:

GNYKEAKKII ; ETPGIRARPT ; RLQWFCDRCI ; ASNQVEALEK ; LVELTEKLFE ;

1000:

CDRDQMYYNL ; LKLYKISSDW ; QRADAAWTKM ; QEENIIPRER ; TLRLLAEILK ;

1050:

TSNQEVPFDV ; PELWFGDDRP ; SLSPSSRSAG ; EDVTEKTLLS ; NCKLKKSKDA ;

1100:

YNIFLKAEKQ ; NVVFSSETYS ; TLIGLLLSKD ; DFTQAMHVKD ; FAETHIKGFT ;

1150:

LNDAANSLLI ; IRQVRRDYLK ; GALATLRAAL ; DLKQVPSQIA ; VTRLIQALAL ;

1200:

KGDVESIEAI ; QRMVAGLDTI ; GLSKMVFINN ; IALAQMKNNK ; LDAAIENIEH ;

1250:

LLASENQAIE ; PQYFGLSYLF ; RKVIEEQMEP ; ALEKLSIMSE ; RMANQFALYK ;

1300:

PVTDLFLQLV ; DSGKVDEARA ; LLERCGAIAE ; QSSLLSVFCL ; RTSQKPKKAP ;

1350:

VLKTLLELIP ; ELRDNDKVYS ; CSMKSYALDK ; DVASAKALYE ; YLTAKNLKLD ;

1392:

DLFLKRYAAL ; LKDVGEPVPF ; PEPPESFAFY ; IKQLKEARES ; PS

Sequence Length

1392

String

Q6PB66

Sub Unit

Interacts with CECR2, HEBP2, MAP1S and UXT (By similarity). Interacts with PPARGC1A (By similarity). Interacts with FOXO1. Component of mRNP complexes associated with HNRPA1.

Uniprot	Q6PB66
Gene Name	Lrpprc
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:72416
Unigene	Mm.217027

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BHE13806860	E14393m	96T	$776		Add to Cart

ELISA Kit for Mouse Leucine-rich PPR motif-containing protein, mitochondrial

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