ELISA Kit for Mouse Kynurenine 3-monooxygenase

Mfr.No.

E13545m

Alternative Names

Kynurenine 3-hydroxylase

Categories

Elisa

Function

Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract.

Specificity

Natural and recombinant Mouse Kynurenine 3-monooxygenase

Subcellular Location

Mitochondrion outer membrane Multi-pass membrane protein

Detection Range

0.78-50ng/mL

Entryname

KMO_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KMO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-107%	93-110%	96-105%	98-104%
EDTA plasma(n=5)	90-108%	92-106%	91-107%	92-105%
heparin plasma(n=5)	93-104%	94-106%	93-104%	94-106%

Method Type

Sandwich

Platform

PF01494

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.4%

Protein Name

Kynurenine 3-monooxygenase

Recovery

Recovery was determined by spiking various levels of KMO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	92-106
Plasma	99	95-105

Sensitivity

0.41ng/mL

Sequence

50:

MASSDTQGKR ; VAVIGGGLVG ; ALNACFLAKR ; NFQVDVYEAR ; EDIRVAKSAR ;

100:

GRSINLALSY ; RGRQALKAIG ; LEDQIVSKGV ; PMKARMIHSL ; SGKKSAIPYG ;

150:

NKSQYILSIS ; RENLNKDLLT ; AVESYANAKV ; HFGHKLSKCI ; PEEGVLTVLG ;

200:

PDKVPRDVTC ; DLVVGCDGAY ; STVRAHLMKK ; PRFDYTQQYI ; PHGYMELTIP ;

250:

PKNGEYAMEP ; NCLHIWPRNA ; YMMIALPNMD ; KSFTCTLFMP ; FEEFERLPTR ;

300:

SDVLDFFQKN ; FPDAIPLMGE ; QALMRDFFLL ; PAQPMISVKC ; SPFHLKSHCV ;

350:

LMGDAAHAIV ; PFFGQGMNAG ; FEDCLVFDEL ; MDKFNNNLSM ; CLPEFSRFRI ;

400:

PDDHAISDLS ; MYNYIEMRAH ; VNSRWFLFQK ; LLDKFLHAIM ; PSTFIPLYTM ;

450:

VAFTRIRYHE ; AVLRWHWQKK ; VINRGLFVLG ; SLIAIGGTYL ; LVHHLSLRPL ;

479:

EFLRRPAWMG ; TTGYWTRSTD ; ISLQVPWSY

Sequence Length

479

String

Q91WN4

Uniprot	Q91WN4
Gene Name	Kmo
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:98256
Unigene	Mm.27217

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BHE13806202	E13545m	96T	$776		Add to Cart

ELISA Kit for Mouse Kynurenine 3-monooxygenase

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