Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
Specificity
Natural and recombinant Mouse Kinase suppressor of Ras 1
Subcellular Location
Cytoplasm Membrane Peripheral membrane protein Endoplasmic reticulum membrane In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. Interacts with VRK2.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);INTERACTION WITH HSPCA; YWHAB; CDC37 AND MAP2K
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1 AND 2);INTERACTION WITH MAPK;MUTAGENESIS OF GLY-572; ARG-589; ARG-615; ASP-700 AND CYS-809;TISSUE SPECIFICITY
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 49-474 (ISOFORMS 1/2)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MARK3;PHOSPHORYLATION AT SER-297 AND SER-392;MUTAGENESIS OF SER-297; SER-392; ILE-397 AND VAL-401
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PPP2R1A AND PPP2CA;DEPHOSPHORYLATION BY PPP2CA
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 331-378 IN COMPLEX WITH ZINC
Reviews of ELISA Kit for Mouse Kinase suppressor of Ras 1