ELISA Kit for Mouse Interleukin-22

Mfr.No.

E2032m

Alternative Names

IL-10-related T-cell-derived-inducible factor ; IL-TIF ; IL-TIF alpha ; Interleukin-22a ; IL-22a ; Il22a ; Iltif ; Iltifa

Categories

Elisa

Function

Cytokine that contributes to the inflammatory response in vivo.

Specificity

Natural and recombinant Mouse Interleukin-22

Subcellular Location

Secreted

Detection Range

15.6-1000pg/mL

Entryname

IL22_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL22 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-105%	93-101%	97-105%	94-107%
EDTA plasma(n=5)	93-101%	97-105%	97-103%	96-106%
heparin plasma(n=5)	96-104%	94-109%	95-101%	97-110%

Method Type

Sandwich

Platform

PF00726

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.9%

Protein Name

Interleukin-22

Recovery

Recovery was determined by spiking various levels of IL22 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	97-104
Plasma	101	95-106

Sensitivity

7.8pg/mL

Sequence

50:

MAVLQKSMSF ; SLMGTLAASC ; LLLIALWAQE ; ANALPVNTRC ; KLEVSNFQQP ;

100:

YIVNRTFMLA ; KEASLADNNT ; DVRLIGEKLF ; RGVSAKDQCY ; LMKQVLNFTL ;

150:

EDVLLPQSDR ; FQPYMQEVVP ; FLTKLSNQLS ; SCHISGDDQN ; IQKNVRRLKE ;

179:

TVKKLGESGE ; IKAIGELDLL ; FMSLRNACV

Sequence Length

179

String

Q9JJY9

Uniprot	Q9JJY9
Gene Name	Il22
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:50929
Unigene	Mm.103585

Reference	1. "Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9." Dumoutier L., Louahed J., Renauld J.-C. J. Immunol.164:1814-1819(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: AKR. 2. "IL-TIF/IL-22: genomic organization and mapping of the human and mouse genes." Dumoutier L., Van Roost E., Colau D., Ameye G., Michaux L., Renauld J.-C. Genes Immun.1:488-494(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE strain: 129. 3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]

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ELISA Kit for Mouse Interleukin-22

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