ELISA Kit for Mouse Interleukin-1 receptor-associated kinase 3

Mfr.No.

E9261m

Alternative Names

IL-1 receptor-associated kinase M ; IRAK-M

Categories

Elisa

Function

Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.

Specificity

Natural and recombinant Mouse Interleukin-1 receptor-associated kinase 3

Detection Range

0.312-20ng/mL

Entryname

IRAK3_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IRAK3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-103%	90-106%	91-105%	92-101%
Saliva(n=3)	90-104%	93-105%	89-106%	90-106%
heparin plasma(n=5)	93-104%	94-102%	95-101%	91-104%

Method Type

Sandwich

Platform

PF00531

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

Protein Name

Interleukin-1 receptor-associated kinase 3

Recovery

Recovery was determined by spiking various levels of IRAK3 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-103
Plasma	98	93-106

Sensitivity

0.171ng/mL

Sequence

50:

MAGRCGARGA ; LSPQLLLFDL ; PPALLGELCG ; ILDSCDGPLG ; WRGLAERLSN ;

100:

SWLDVRHIEK ; YVNQGKSGTR ; ELLWSWAQKN ; KTIGDLLEVL ; QDMGHQRAIH ;

150:

LIINYGVSWT ; PSVQTHHELP ; FPSFPPEVKH ; ACRENDPGPL ; EPANVTVDNV ;

200:

LVPEHNEKGT ; LQKTPISFQS ; ILEGTKHFHK ; DFLIGEGEIF ; EVYRVDIRNQ ;

250:

AYAVKLFKQE ; KKMQLKKHWK ; RFLSELEVLL ; LFRHPHILEL ; AAYFTETEKL ;

300:

CLVYPYMSNG ; TLFDRLQCTN ; GTTPLSWHVR ; ISVLIGIAKA ; IQYLHNTQPC ;

350:

AVICGNVSSA ; NILLDDQLQP ; KLTDFAAAHF ; RPNLEQQSST ; INMTGGGRKH ;

400:

LWYMPEEYIR ; QGRLSVKTDV ; YSFGIVIMEV ; LTGCKVVLDD ; PKHVQLRDLL ;

450:

MELMEKRGLD ; SCLSFLDRKI ; PPCPRNFSAK ; LFSLAGRCVA ; TKAKLRPTMD ;

500:

EVLSSLESTQ ; PSLYFAEDPP ; TSLKSFRCPS ; PLFLDNVPSI ; PVEDDENQNN ;

550:

HSVPPKEVLG ; TDRVTQKTPF ; ECSQSEVTFL ; GLDRNRGNRG ; SEADCNVPSS ;

600:

SHEECWSPEL ; VAPSQDLSPT ; VISLGSSWEV ; PGHSYGSKPM ; EKRCSSGLFC ;

609:

SEHEQSKKQ

Sequence Length

609

String

Q8K4B2

Uniprot	Q8K4B2
Gene Name	Irak3
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:73914
Unigene	Mm.146194

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SKU	Supplier No.	Size	Your price	Quantity
BHE13806070	E9261m	96T	$776		Add to Cart

ELISA Kit for Mouse Interleukin-1 receptor-associated kinase 3

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