ELISA Kit for Mouse Interleukin-1 receptor-associated kinase 1

Mfr.No.

E1514m

Alternative Names

Pelle-like protein kinase ; mPLK ; Il1rak

Categories

Elisa

Function

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-TAK1/NR2C2-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, TAK1/NR2C2 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

Specificity

Natural and recombinant Mouse Interleukin-1 receptor-associated kinase 1

Subcellular Location

Cytoplasm Nucleus Translocates to the nucleus when sumoylated.

Detection Range

78-5000pg/mL

Entryname

IRAK1_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IRAK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	99-110%	102-110%	101-109%	99-109%
EDTA plasma(n=5)	98-113%	99-107%	96-112%	97-108%
heparin plasma(n=5)	100-107%	97-110%	102-107%	100-106%

Method Type

Sandwich

Platform

PF00531

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.3%

Protein Name

Interleukin-1 receptor-associated kinase 1

Recovery

Recovery was determined by spiking various levels of IRAK1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	105	100-110
Plasma	104	102-112

Sensitivity

40pg/mL

Sequence

50:

MAGGPGPGEP ; VVPGAQHFLY ; EVPPWVMCRF ; YKVMDALEPA ; DWCQFAALIV ;

100:

RDQTELRLCE ; RSEQRTASVL ; WPWINRNARV ; ADLVHILTHL ; QLLRARDIIT ;

150:

AWHPPAPVVP ; PSTAAPRPSS ; ISAGSEAGDW ; SPRKLQSSAS ; TFLSPAFPGS ;

200:

QTHSESELLQ ; VPLPVSLGPP ; LPSSAPSSTK ; SSPESPVSGL ; QRAHPSPFCW ;

250:

PFCEISQGTC ; NFSEELRIGE ; GGFGCVYRAV ; MRNTTYAVKR ; LKEEADLEWT ;

300:

MVKQSFLTEV ; EQLSRFRHPN ; IVDFAGYCAE ; SGLYCLVYGF ; LPNGSLEDQL ;

350:

HLQTQACSPL ; SWPQRLDILL ; GTARAIQFLH ; QDSPSLIHGD ; IKSSNVLLDE ;

400:

RLMPKLGDFG ; LARFSRFAGA ; KASQSSTVAR ; TSTVRGTLAY ; LPEEYIKTGR ;

450:

LAVDTDTFSF ; GVVILETLAG ; QRAVRTQGAK ; TKYLKDLIED ; EAEEAGVTLK ;

500:

STQPTLWVGV ; ATDAWAAPIA ; AQIYKKHLDS ; RPGPCPPQLG ; LALAQLACCC ;

550:

MHRRAKKRPP ; MTQVYKRLEG ; LQAGPPWELE ; VAGHGSPSPQ ; ENSYMSTTGS ;

600:

AQSGDEPWQP ; LVVTTRAPAQ ; AAQQLQRSPN ; QPVESDESVP ; GLSATLHSWH ;

650:

LTPGSHPSPA ; SFREASCTQG ; GTTRESSVRS ; SPGFQPTTME ; GSPTGSSSLL ;

700:

SSEPPQIIIN ; PARQKMVQKL ; ALYEEGVLDS ; LQLLSSGFFP ; GLDLEPEKSQ ;

710:

GPEESDEFQS ;

Sequence Length

710

String

Q62406

Sub Unit

Homodimer (By similarity). Interacts with TOLLIP; this interaction occurs in the cytosol prior to receptor activation. Interacts with MYD88; this interaction recruits IRAK1 to the stimulated receptor complex. Interacts with IL1RL1. Interacts with IRAK1BP1 (By similarity). Associates with TRAF6, PELI1 and IRAK4; this complex recruits TAK1/NR2C2, TAB1 and TAB2 to mediate NF-kappa-B activation. Interacts (when polyubiquitinated) with IKBKG/NEMO.

Uniprot	Q62406
Gene Name	Irak1
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:16179
Unigene	Mm.38241

Reference	1. "Cloning of mouse IRAK." Kopp E.B., Ghosh S. Submitted (1998-11) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1) 2. "Comparative sequence analysis of the MECP2-locus in human and mouse reveals new transcribed regions." Reichwald K., Thiesen J., Wiehe T., Weitzel J., Poustka W.A., Rosenthal A., Platzer M., Stratling W.H., Kioschis P. Mamm. Genome11:182-190(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2) 3. "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.more... PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] strain: C57BL/6J. 4. "Developmental and tissue-specific expression of mouse pelle-like protein kinase." Trofimova M., Sprenkle A.B., Green M., Sturgill T.W., Goebl M.G., Harrington M.A. J. Biol. Chem.271:17609-17612(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 34-710 (ISOFORM 1);FUNCTION;DEVELOPMENTAL STAGE;TISSUE SPECIFICITY;AUTOPHOSPHORYLATION tissue: Embryo. 5. "SIMPL is a tumor necrosis factor-specific regulator of nuclear factor-kappaB activity." Vig E., Green M., Liu Y., Yu K.-Y., Kwon H.-J., Tian J., Goebl M.G., Harrington M.A. J. Biol. Chem.276:7859-7866(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH IRAK1BP1 6. "Identification of threonine 66 as a functionally critical residue of the interleukin-1 receptor-associated kinase." Ross K., Yang L., Dower S., Volpe F., Guesdon F. J. Biol. Chem.277:37414-37421(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DIMERIZATION;MUTAGENESIS OF THR-66 7. "Mouse pellino-2 modulates IL-1 and lipopolysaccharide signaling." Yu K.-Y., Kwon H.-J., Norman D.A.M., Vig E., Goebl M.G., Harrington M.A. J. Immunol.169:4075-4078(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PELI2 8. "Interleukin-1 receptor-associated kinase-1 plays an essential role for Toll-like receptor (TLR)7- and TLR9-mediated interferon-{alpha} induction." Uematsu S., Sato S., Yamamoto M., Hirotani T., Kato H., Takeshita F., Matsuda M., Coban C., Ishii K.J., Kawai T., Takeuchi O., Akira S. J. Exp. Med.201:915-923(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE 9. "Smad6 negatively regulates interleukin 1-receptor-Toll-like receptor signaling through direct interaction with the adaptor Pellino-1." Choi K.C., Lee Y.S., Lim S., Choi H.K., Lee C.H., Lee E.K., Hong S., Kim I.H., Kim S.J., Park S.H. Nat. Immunol.7:1057-1065(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN COMPLEX WITH IRAK4; MYD88; PELI1 AND TRAF6 10. "Antiviral protein Viperin promotes Toll-like receptor 7- and Toll-like receptor 9-mediated type I interferon production in plasmacytoid dendritic cells." Saitoh T., Satoh T., Yamamoto N., Uematsu S., Takeuchi O., Kawai T., Akira S. Immunity34:352-363(2011) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH RSAD2;SUBCELLULAR LOCATION

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ELISA Kit for Mouse Interleukin-1 receptor-associated kinase 1

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