ELISA Kit for Mouse Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein

Mfr.No.

E11526m

Categories

Elisa

Function

Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins.

Specificity

Natural and recombinant Mouse Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein

Subcellular Location

Endoplasmic reticulum membrane Multi-pass membrane protein

Detection Range

0.312-20ng/ml

Entryname

HERP1_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HERP1and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	99-109%	97-111%	95-115%	98-112%
EDTA plasma(n=5)	93-110%	95-110%	92-113%	93-109%
heparin plasma(n=5)	97-108%	94-112%	96-108%	96-107%

Method Type

Sandwich

Platform

PF00240

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.9%

Protein Name

Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein

Recovery

Recovery was determined by spiking various levels of HERP1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	104	95-107
Plasma	102	96-105

Sensitivity

0.156ng/mL

Sequence

50:

MEPEPQPEPV ; TLLVKSPNQR ; HRDLELSGDR ; SWSVSRLKAH ; LSRVYPERPR ;

100:

PEDQRLIYSG ; KLLLDHQCLQ ; DLLPKQEKRH ; VLHLVCNVKN ; PSKMPETSTK ;

150:

GAESTEQPDN ; SNQTQHPGDS ; SSDGLRQREV ; LRNLSPSGWE ; NISRPEAVQQ ;

200:

TFQGLGPGFS ; GYTTYGWLQL ; SWFQQIYARQ ; YYMQYLAATA ; ASGTFVPTPS ;

250:

AQEIPVVSTP ; APAPIHNQFP ; AENQPANQNA ; AAQAVVNPGA ; NQNLRMNAQG ;

300:

GPLVEEDDEI ; NRDWLDWTYS ; AATFSVFLSI ; LYFYSSLSRF ; LMVMGATVVM ;

350:

YLHHVGWFPF ; RQRPVQNFPD ; DGGPRDAANQ ; DPNNNLQGGM ; DPEMEDPNRL ;

391:

PPDREVLDPE ; HTSPSFMSTA ; WLVFKTFFAS ; LLPEGPPALA ; N

Sequence Length

391

String

Q9JJK5

Sub Unit

Interacts with PSEN1 and PSEN2. Interacts with SYVN1 and UBXN6.

Uniprot	Q9JJK5
Gene Name	Herpud1
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:64209
Unigene	Mm.29151

Reference	1. "Herp, a new ubiquitin-like membrane protein induced by endoplasmic reticulum stress." Kokame K., Agarwala K.L., Kato H., Miyata T. J. Biol. Chem.275:32846-32853(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]

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SKU	Supplier No.	Size	Your price	Quantity
BHE13805211	E11526m	96T	$776		Add to Cart

ELISA Kit for Mouse Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein

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