ELISA Kit for Mouse HMG box-containing protein 1

Mfr.No.

E6776m

Alternative Names

HMG box transcription factor 1 ; High mobility group box transcription factor 1

Categories

Elisa

Function

Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.

Specificity

Natural and recombinant Mouse HMG box-containing protein 1

Subcellular Location

Nucleus

Detection Range

0.156-10ng/mL

Entryname

HBP1_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HBP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-104%	90-105%	95-100%	93-101%
EDTA plasma(n=5)	90-107%	93-105%	91-104%	90-106%
heparin plasma(n=5)	93-104%	94-102%	92-102%	91-105%

Method Type

Sandwich

Platform

PF08517

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

Protein Name

HMG box-containing protein 1

Recovery

Recovery was determined by spiking various levels of HBP1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-103
Plasma	98	93-106

Sensitivity

0.083ng/mL

Sequence

50:

MVWEVKTNQM ; PNAVQKLLLV ; MDKRAPGMSD ; SLELLQCNEN ; LPSSPGYNSC ;

100:

DEHMELDDLP ; ELQAVQSDPT ; QSAIYQLSSD ; VSHQEYPRSS ; WSQNTSDIPE ;

150:

NTHREDEVDW ; LTELANIATS ; PQSPLMQCSF ; YNRSSPVHII ; ATSKSLHSYA ;

200:

RPPPVSSSSK ; SGPAFPHDHW ; KEETPVRHER ; ANSESESGIF ; CMSSLSDDDD ;

250:

LGWCNSWPST ; IWHCFLKGTR ; LCFHKESNKE ; WQDVEDFARA ; ASCDNEEEIQ ;

300:

MGTHKGYGSD ; GLKLLSHEES ; VSFGESVLKL ; TFDPGTVEDG ; LLTVECKLDH ;

350:

PFYVKNKGWS ; SFYPSLTVVQ ; HGIPCCEIHI ; GDVCLPPGHP ; DAINFDDSGV ;

400:

FDTFKSYDFT ; PMDSSAVYVL ; SSMARQRRAS ; LSCGGGPGTG ; QEFSGSEFSK ;

450:

SCGSPGSSQL ; SSSSLYAKAV ; KSHSSGTVSA ; TSPNKCKRPM ; NAFMLFAKKY ;

500:

RVEYTQMYPG ; KDNRAISVIL ; GDRWKKMKNE ; ERRMYTLEAK ; ALAEEQKRLN ;

516:

PDCWKRKRTN ; SGSQQH

Sequence Length

516

String

Q8R316

Sub Unit

Binds TCF4 (By similarity). Binds RB1. Binds the second PAH repeat of SIN3A.

Uniprot	Q8R316
Gene Name	Hbp1
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:73389
Unigene	Mm.390461

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SKU	Supplier No.	Size	Your price	Quantity
BHE13804691	E6776m	96T	$776		Add to Cart

ELISA Kit for Mouse HMG box-containing protein 1

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