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ELISA Kit for Mouse GTPase HRas

SKU : BHE13809883

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E1294m
Alternative Names	H-Ras-1 ; Transforming protein p21 ; c-H-ras ; p21ras ; Hras1
Categories	Elisa
Function	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Specificity	Natural and recombinant Mouse GTPase HRas
Subcellular Location	Cell membrane Cell membrane Lipid-anchor Cytoplasmic side Golgi apparatus Golgi apparatus membrane Lipid-anchor Shuttles between the plasma membrane and the Golgi apparatus. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form.
Detection Range	0.156-10ng/ml
Entryname	RASH_MOUSE
Method Type	Sandwich
Platform	PF00071
Protein Name	GTPase HRas
Sensitivity	0.081ng/mL
Sequence	50: MTEYKLVVVG ; AGGVGKSALT ; IQLIQNHFVD ; EYDPTIEDSY ; RKQVVIDGET ; 100: CLLDILDTAG ; QEEYSAMRDQ ; YMRTGEGFLC ; VFAINNTKSF ; EDIHQYREQI ; 150: KRVKDSDDVP ; MVLVGNKCDL ; AARTVESRQA ; QDLARSYGIP ; YIETSAKTRQ ; 189: GVEDAFYTLV ; REIRQHKLRK ; LNPPDESGPG ; CMSCKCVLS
Sequence Length	189
String	Q61411
Sub Unit	Forms a signaling complex with RASGRP1 and DGKZ. In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C and RASSF5. Interacts with PDE6D. Interacts with IKZF3. Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3 and RGS14. Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain). Interacts with GNB2L1 (By similarity). Interacts with RGL3. Interacts with HSPD1.

Uniprot	Q61411
Gene Name	Hras
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:15461
Unigene	Mm.334313

Reference	1. "Nucleotide sequence of c-H-ras-1 gene from B6C3F1 mice." Przybojewska B., Plucienniczak G. Acta Biochim. Pol.43:575-578(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] strain: C57BL/6 X C3H. tissue: Liver. 2. Lin L., Fu D. Submitted (2003-08) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1) strain: BALB/c. tissue: Keratinocyte. 3. "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.more... PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] 4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (2005-07) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] 5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1) tissue: Olfactory epithelium. 6. "Spontaneous mutation at codon 61 of the Ha-ras gene in the nascent liver of B6C3F1, C3H/He and C57BL/6 mice." Moulds B.A., Goodman J.I. Mutat. Res.311:1-7(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 59-75;MUTAGENESIS OF GLN-61 7. Lubec G., Kang S.U. Submitted (2007-04) to the UniProtKB [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 136-147;IDENTIFICATION BY MASS SPECTROMETRY strain: C57BL/6. tissue: Brain. 8. "Alternative splicing of the human proto-oncogene c-H-ras renders a new Ras family protein that trafficks to cytoplasm and nucleus." Guil S., de La Iglesia N., Fernandez-Larrea J., Cifuentes D., Ferrer J.C., Guinovart J.J., Bach-Elias M. Cancer Res.63:5178-5187(2003) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 151-189 (ISOFORM 2);ALTERNATIVE SPLICING strain: NIH/3T3. 9. "An interaction between p21ras and heat shock protein hsp60, a chaperonin." Ikawa S., Weinberg R.A. Proc. Natl. Acad. Sci. U.S.A.89:2012-2016(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HSPD1 10. "A novel RalGEF-like protein, RGL3, as a candidate effector for rit and Ras." Shao H., Andres D.A. J. Biol. Chem.275:26914-26924(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH RGL3 11. "Ras is an indispensable coregulator of the class IB phosphoinositide 3-kinase p87/p110gamma." Kurig B., Shymanets A., Bohnacker T., Prajwal X., Brock C., Ahmadian M.R., Schaefer M., Gohla A., Harteneck C., Wymann M.P., Jeanclos E., Nurnberg B. Proc. Natl. Acad. Sci. U.S.A.106:20312-20317(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PIK3CG

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