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ELISA Kit for Mouse Endoplasmin

SKU : BHE13803522

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E0823m
Alternative Names	94 kDa glucose-regulated protein ; GRP-94 ; Endoplasmic reticulum resident protein 99 ; ERp99 ; Heat shock protein 90 kDa beta member 1 ; Polymorphic tumor rejection antigen 1 ; Tumor rejection antigen gp96 ; Grp94 ; Tra-1 ; Tra1
Categories	Elisa
Function	Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
Specificity	Natural and recombinant Mouse Endoplasmin
Subcellular Location	Endoplasmic reticulum lumen Melanosome
Detection Range	0.156-10ng/mL
Entryname	ENPL_MOUSE
Method Type	Sandwich
Platform	PF00183
Protein Name	Endoplasmin
Sensitivity	0.081ng/mL
Sequence	50: MRVLWVLGLC ; CVLLTFGFVR ; ADDEVDVDGT ; VEEDLGKSRE ; GSRTDDEVVQ ; 100: REEEAIQLDG ; LNASQIRELR ; EKSEKFAFQA ; EVNRMMKLII ; NSLYKNKEIF ; 150: LRELISNASD ; ALDKIRLISL ; TDENALAGNE ; ELTVKIKCDK ; EKNLLHVTDT ; 200: GVGMTREELV ; KNLGTIAKSG ; TSEFLNKMTE ; AQEDGQSTSE ; LIGQFGVGFY ; 250: SAFLVADKVI ; VTSKHNNDTQ ; HIWESDSNEF ; SVIADPRGNT ; LGRGTTITLV ; 300: LKEEASDYLE ; LDTIKNLVRK ; YSQFINFPIY ; VWSSKTETVE ; EPLEEDEAAK ; 350: EEKEESDDEA ; AVEEEEEEKK ; PKTKKVEKTV ; WDWELMNDIK ; PIWQRPSKEV ; 400: EEDEYKAFYK ; SFSKESDDPM ; AYIHFTAEGE ; VTFKSILFVP ; TSAPRGLFDE ; 450: YGSKKSDYIK ; LYVRRVFITD ; DFHDMMPKYL ; NFVKGVVDSD ; DLPLNVSRET ; 500: LQQHKLLKVI ; RKKLVRKTLD ; MIKKIADEKY ; NDTFWKEFGT ; NIKLGVIEDH ; 550: SNRTRLAKLL ; RFQSSHHSTD ; ITSLDQYVER ; MKEKQDKIYF ; MAGSSRKEAE ; 600: SSPFVERLLK ; KGYEVIYLTE ; PVDEYCIQAL ; PEFDGKRFQN ; VAKEGVKFDE ; 650: SEKTKESREA ; TEKEFEPLLN ; WMKDKALKDK ; IEKAVVSQRL ; TESPCALVAS ; 700: QYGWSGNMER ; IMKAQAYQTG ; KDISTNYYAS ; QKKTFEINPR ; HPLIRDMLRR ; 750: IKEDEDDKTV ; MDLAVVLFET ; ATLRSGYLLP ; DTKAYGDRIE ; RMLRLSLNID ; 800: PEAQVEEEPE ; EEPEDTSEDA ; EDSEQDEGEE ; MDAGTEEEEE ; ETEKESTEKD ; 802: EL
Sequence Length	802
String	P08113
Sub Unit	Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with OS9 (By similarity). Interacts with AIMP1; regulates its retention in the endoplasmic reticulum.

Uniprot	P08113
Gene Name	Hsp90b1
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:22027
Unigene	Mm.87773

Reference	1. "ERp99, an abundant, conserved glycoprotein of the endoplasmic reticulum, is homologous to the 90-kDa heat shock protein (hsp90) and the 94-kDa glucose regulated protein (GRP94)." Mazzarella R.A., Green M. J. Biol. Chem.262:8875-8883(1987) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. Lubec G., Kang S.U. Submitted (2007-04) to the UniProtKB [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 76-84; 88-95; 117-135; 143-156; 244-265; 270-285; 385-404; 416-428; 435-448; 512-530; 609-623 AND 640-660;IDENTIFICATION BY MASS SPECTROMETRY strain: C57BL/6. tissue: Brain. 3. "Isolation and identification of partial cDNA clones for endoplasmin, the major glycoprotein of mammalian endoplasmic reticulum." Smith M.J., Koch G.L.E. J. Mol. Biol.194:345-347(1987) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 378-507 4. "5'-structural analysis of genes encoding polymorphic antigens of chemically induced tumors." Srivastava P.K., Chen Y.-T., Old L.J. Proc. Natl. Acad. Sci. U.S.A.84:3807-3811(1987) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-88 5. "HSP100, a 100-kDa heat shock protein, is a Ca2+-calmodulin-regulated actin-binding protein." Koyasu S., Nishida E., Miyata Y., Sakai H., Yahara I. J. Biol. Chem.264:15083-15087(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 271-284; 328-339 AND 633-659 6. "Analysis of the structure and synthesis of GRP94, an abundant stress protein of the endoplasmic reticulum." Qu D., Mazzarella R.A., Green M. DNA Cell Biol.13:117-124(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERCHAIN DISULFIDE BOND 7. "A subset of chaperones and folding enzymes form multiprotein complexes in endoplasmic reticulum to bind nascent proteins." Meunier L., Usherwood Y.-K., Chung K.T., Hendershot L.M. Mol. Biol. Cell13:4456-4469(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: COMPONENT OF A CHAPERONE COMPLEX 8. "Age-specific CUGBP1-eIF2 complex increases translation of CCAAT/enhancer-binding protein beta in old liver." Timchenko L.T., Salisbury E., Wang G.-L., Nguyen H., Albrecht J.H., Hershey J.W., Timchenko N.A. J. Biol. Chem.281:32806-32819(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN AN EIF2 COMPLEX WITH EIF2S1; EIF2S2; CELF1; CALR3; HSPA5 AND CALR 9. "Mzb1 protein regulates calcium homeostasis, antibody secretion, and integrin activation in innate-like B cells." Flach H., Rosenbaum M., Duchniewicz M., Kim S., Zhang S.L., Cahalan M.D., Mittler G., Grosschedl R. Immunity33:723-735(2010) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MZB1 10. "Folding of Toll-like receptors by the HSP90 paralogue gp96 requires a substrate-specific cochaperone." Liu B., Yang Y., Qiu Z., Staron M., Hong F., Li Y., Wu S., Li Y., Hao B., Bona R., Han D., Li Z. Nat. Commun.1:79-79(2010) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH CNPY3; TLR4; TLR9 AND TLR11;SUBCELLULAR LOCATION;MUTAGENESIS OF GLU-103 11. Liu B., Yang Y., Qiu Z., Staron M., Hong F., Li Y., Wu S., Li Y., Hao B., Bona R., Han D., Li Z. Nat. Commun.3:653-653(2012) [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM 12. "Aminoacyl-tRNA synthetase-interacting multifunctional protein 1/p43 controls endoplasmic reticulum retention of heat shock protein gp96: its pathological implications in lupus-like autoimmune diseases." Han J.M., Park S.G., Liu B., Park B.-J., Kim J.Y., Jin C.H., Song Y.W., Li Z., Kim S. Am. J. Pathol.170:2042-2054(2007) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH AIMP1 13. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol.27:378-386(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-217 14. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways." Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y. Mol. Cell50:919-930(2013) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-479;SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-404 AND LYS-633;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS] tissue: Embryonic fibroblast.

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