ELISA Kit for Mouse DNA topoisomerase 1

Mfr.No.

E9586m

Alternative Names

DNA topoisomerase I ; Top-1

Categories

Elisa

Function

The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

Specificity

Natural and recombinant Mouse DNA topoisomerase 1

Subcellular Location

Nucleus nucleolus Nucleus nucleoplasm Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.

Detection Range

0.156-10ng/mL

Entryname

TOP1_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-102%	91-99%	91-104%	89-99%
EDTA plasma(n=5)	87-96%	93-98%	89-95%	90-102%
heparin plasma(n=5)	88-101%	89-102%	91-107%	94-110%

Method Type

Sandwich

Platform

PF14370

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 7.4%

Protein Name

DNA topoisomerase 1

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	95-102
Plasma	94	93-99

Sensitivity

0.078ng/mL

Sequence

50:

MSGDHLHNDS ; QIEADFRLND ; SHKHKDKHKD ; REHRHKEHKK ; DKDKDREKSK ;

100:

HSNSEHKDSE ; KKHKEKEKTK ; HKDGSSEKHK ; DKHKDRDKER ; RKEEKIRAAG ;

150:

DAKIKKEKEN ; GFSSPPRIKD ; EPEDDGYFAP ; PKEDIKPLKR ; LRDEDDADYK ;

200:

PKKIKTEDIK ; KEKKRKSEEE ; EDGKLKKPKN ; KDKDKKVAEP ; DNKKKKPKKE ;

250:

EEQKWKWWEE ; ERYPEGIKWK ; FLEHKGPVFA ; PPYEPLPESV ; KFYYDGKVMK ;

300:

LSPKAEEVAT ; FFAKMLDHEY ; TTKEIFRKNF ; FKDWRKEMTN ; DEKNTITNLS ;

350:

KCDFTQMSQY ; FKAQSEARKQ ; MSKEEKLKIK ; EENEKLLKEY ; GFCVMDNHRE ;

400:

RIANFKIEPP ; GLFRGRGNHP ; KMGMLKRRIM ; PEDIIINCSK ; DAKVPSPPPG ;

450:

HKWKEVRHDN ; KVTWLVSWTE ; NIQGSIKYIM ; LNPSSRIKGE ; KDWQKYETAR ;

500:

RLKKCVDKIR ; NQYREDWKSK ; EMKVRQRAVA ; LYFIDKLALR ; AGNEKEEGET ;

550:

ADTVGCCSLR ; VEHINLHPEL ; DGQEYVVEFD ; FPGKDSIRYY ; NKVPVEKRVF ;

600:

KNLQLFMENK ; QPEDDLFDRL ; NTGILNKHLQ ; DLMEGLTAKV ; FRTYNASITL ;

650:

QQQLKELTAP ; DENVPAKILS ; YNRANRAVAI ; LCNHQRAPPK ; TFEKSMMNLQ ;

700:

SKIDAKKDQL ; ADARRDLKSA ; KADAKVMKDA ; KTKKVVESKK ; KAVQRLEEQL ;

750:

MKLEVQATDR ; EENKQIALGT ; SKLNYLDPRI ; TVAWCKKWGV ; PIEKIYNKTQ ;

767:

REKFAWAIDM ; TDEDYEF

Sequence Length

767

String

Q04750

Sub Unit

Monomer.

Uniprot	Q04750
Gene Name	Top1
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:21969
Unigene	Mm.217233

Reference	1. "Cloning of the mouse cDNA encoding DNA topoisomerase I and chromosomal location of the gene." Koiwai O., Yasui Y., Sakai Y., Watanabe T., Ishii K., Yanagihara S., Andoh T. Gene125:211-216(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE 2. "Cloning and characterization of mouse topoisomerase I cDNA." Hui C.-F., Lo C.K., Hwang J. Submitted (1993-07) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 3. "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.more... PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] strain: C57BL/6J. 4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways." Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y. Mol. Cell50:919-930(2013) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-174;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS] tissue: Embryonic fibroblast.

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ELISA Kit for Mouse DNA topoisomerase 1

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