Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate.
Specificity
Natural and recombinant Mouse Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2
Subcellular Location
Golgi apparatus membrane Single-pass type II membrane protein