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ELISA Kit for Mouse Apoptosis-inducing factor 1, mitochondrial

SKU : BHE13800760

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E1064m
Alternative Names	Programmed cell death protein 8 ; Aif ; Pdcd8
Categories	Elisa
Function	Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis,and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Specificity	Natural and recombinant Mouse Apoptosis-inducing factor 1, mitochondrial
Subcellular Location	Mitochondrion intermembrane space Mitochondrion inner membrane Cytoplasm Cytoplasm perinuclear region Nucleus Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
Detection Range	78-5000pg/ml
Entryname	AIFM1_MOUSE
Method Type	Sandwich
Platform	PF00070
Protein Name	Apoptosis-inducing factor 1, mitochondrial
Sensitivity	40.4pg/mL
Sequence	50: MFRCGGLAGA ; FKQKLVPLVR ; TVYVQRPKQR ; NRLPGNLFQQ ; WRVPLELQMA ; 100: RQMASSGSSG ; GKMDNSVLVL ; IVGLSTIGAG ; AYAYKTIKED ; QKRYNERVMG ; 150: LGLSPEEKQR ; RAIASATEGG ; SVPQIRAPSH ; VPFLLIGGGT ; AAFAAARSIR ; 200: ARDPGARVLI ; VSEDPELPYM ; RPPLSKELWF ; SDDPNVTKTL ; QFRQWNGKER ; 250: SIYFQPPSFY ; VSAQDLPNIE ; NGGVAVLTGK ; KVVHLDVRGN ; MVKLNDGSQI ; 300: TFEKCLIATG ; GTPRSLSAID ; RAGAEVKSRT ; TLFRKIGDFR ; ALEKISREVK ; 350: SITVIGGGFL ; GSELACALGR ; KSQASGIEVI ; QLFPEKGNMG ; KILPQYLSNW ; 400: TMEKVKREGV ; KVMPNAIVQS ; VGVSGGRLLI ; KLKDGRKVET ; DHIVTAVGLE ; 450: PNVELAKTGG ; LEIDSDFGGF ; RVNAELQARS ; NIWVAGDAAC ; FYDIKLGRRR ; 500: VEHHDHAVVS ; GRLAGENMTG ; AAKPYWHQSM ; FWSDLGPDVG ; YEAIGLVDSS ; 550: LPTVGVFAKA ; TAQDNPKSAT ; EQSGTGIRSE ; SETESEASEI ; TIPPSAPAVP ; 600: QVPVEGEDYG ; KGVIFYLRDK ; VVVGIVLWNV ; FNRMPIARKI ; IKDGEQHEDL ; 612: NEVAKLFNIH ; ED
Sequence Length	612
String	Q9Z0X1
Sub Unit	Interacts with XIAP. Interacts (via N-terminus) with EIF3G (via C-terminus).

Uniprot	Q9Z0X1
Gene Name	Aifm1
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:26926
Unigene	Mm.240434

Reference	1. "Molecular characterization of mitochondrial apoptosis-inducing factor." Susin S.A., Lorenzo H.K., Zamzami N., Marzo I., Snow B.E., Brothers G.M., Mangion J., Jacotot E., Costantini P., Loeffler M., Larochette N., Goodlett D.R., Aebersold R., Siderovski D.P., Penninger J.M., Kroemer G. Nature397:441-446(1999) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF N-TERMINUS;PROTEIN SEQUENCE OF 322-336;FUNCTION;COFACTOR;IDENTIFICATION BY MASS SPECTROMETRY;SUBCELLULAR LOCATION 2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: FVB/N. tissue: Mammary gland. 3. Lubec G., Sunyer B., Chen W.-Q. Submitted (2009-01) to the UniProtKB [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 378-386;IDENTIFICATION BY MASS SPECTROMETRY strain: OF1. tissue: Hippocampus. 4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways." Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y. Mol. Cell50:919-930(2013) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-108;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS] tissue: Liver. 5. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways." Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W. Proc. Natl. Acad. Sci. U.S.A.110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-387 AND LYS-592;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS] tissue: Liver. 6. "The crystal structure of the mouse apoptosis-inducing factor AIF." Mate M.J., Ortiz-Lombardia M., Boitel B., Haouz A., Tello D., Susin S.A., Penninger J., Kroemer G., Alzari P.M. Nat. Struct. Biol.9:442-446(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 108-610 IN COMPLEX WITH FAD;NAD-BINDING;MUTAGENESIS OF LYS-176 AND GLU-313

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