ELISA Kit for Mouse Amphiregulin

Mfr.No.

E0006m

Alternative Names

Schwannoma-derived growth factor ; SDGF ; Sdgf

Categories

Elisa

Function

Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

Specificity

Natural and recombinant Mouse Amphiregulin

Subcellular Location

Membrane Single-pass membrane protein

Detection Range

15.6-1000pg/mL

Entryname

AREG_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LRP8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	94-105%	97-103%	95-104%	92-109%
EDTA plasma(n=5)	92-99%	93-105%	93-101%	96-106%
heparin plasma(n=5)	93-105%	96-110%	98-109%	98-110%

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.2%

Protein Name

Amphiregulin

Recovery

Recovery was determined by spiking various levels of LRP8 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	94-105
Plasma	98	93-104

Sensitivity

7.82pg/mL

Sequence

50:

MRTPLLPLAR ; SVLLLLVLGS ; GHYAAALELN ; DPSSGKGESL ; SGDHSAGGLE ;

100:

LSVGREVSTI ; SEMPSGSELS ; TGDYDYSEEY ; DNEPQISGYI ; IDDSVRVEQV ;

150:

IKPKKNKTEG ; EKSTEKPKRK ; KKGGKNGKGR ; RNKKKKNPCT ; AKFQNFCIHG ;

200:

ECRYIENLEV ; VTCNCHQDYF ; GERCGEKSMK ; THSEDDKDLS ; KIAVVAVTIF ;

248:

VSAIILAAIG ; IGIVITVHLW ; KRYFREYEGE ; TEERRRLRQE ; NGTVHAIA

Sequence Length

248

String

P31955

Sub Unit

The immature precursor interacts with CNIH.

Uniprot	P31955
Gene Name	Areg
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:11839
Unigene	Mm.8039

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SKU	Supplier No.	Size	Your price	Quantity
BHE13801206	E0006m	96T	$776		Add to Cart

ELISA Kit for Mouse Amphiregulin

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