ELISA Kit for Mouse Amelogenin, X isoform

Mfr.No.

E14172m

Alternative Names

Leucine-rich amelogenin peptide ; LRAP ; Amel

Categories

Elisa

Function

Plays a role in the biomineralization of teeth. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel.

Specificity

Natural and recombinant Mouse Amelogenin, X isoform

Subcellular Location

Secreted extracellular space extracellular matrix

Detection Range

0.156-10ng/mL

Entryname

AMELX_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMELX and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-112%	97-109%	95-111%	99-107%
EDTA plasma(n=5)	99-105%	100-107%	97-109%	94-112%
heparin plasma(n=5)	95-110%	98-106%	96-111%	98-108%

Method Type

Sandwich

Platform

PF02948

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.1%

Protein Name

Amelogenin, X isoform

Recovery

Recovery was determined by spiking various levels of AMELX into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	104	97-108
Plasma	103	95-106

Sensitivity

0.079ng/mL

Sequence

50:

MGTWILFACL ; LGAAFAMPLP ; PHPGSPGYIN ; LSYEKSHSQA ; INTDRTALVL ;

100:

TPLKWYQSMI ; RQPYPSYGYE ; PMGGWLHHQI ; IPVLSQQHPP ; SHTLQPHHHL ;

150:

PVVPAQQPVA ; PQQPMMPVPG ; HHSMTPTQHH ; QPNIPPSAQQ ; PFQQPFQPQA ;

200:

IPPQSHQPMQ ; PQSPLHPMQP ; LAPQPPLPPL ; FSMQPLSPIL ; PELPLEAWPA ;

210:

TDKTKREEVD ;

Sequence Length

210

String

P63277

Uniprot	P63277
Gene Name	Amelx
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:11704
Unigene	Mm.391342

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SKU	Supplier No.	Size	Your price	Quantity
BHE13800592	E14172m	96T	$776		Add to Cart

ELISA Kit for Mouse Amelogenin, X isoform

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