ELISA Kit for Mouse Adenosine receptor A1

Mfr.No.

E12273m

Categories

Elisa

Function

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.

Specificity

Natural and recombinant Mouse Adenosine receptor A1

Subcellular Location

Cell membrane Multi-pass membrane protein

Detection Range

78-5000pg/mL

Entryname

AA1R_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AA1R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	94-108%	97-106%	95-106%	92-110%
EDTA plasma(n=5)	92-107%	95-105%	91-108%	94-105%
heparin plasma(n=5)	95-103%	93-108%	93-107%	90-110%

Method Type

Sandwich

Platform

PF00001

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.2%

Protein Name

Adenosine receptor A1

Recovery

Recovery was determined by spiking various levels of AA1R into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	93-104
Plasma	100	92-106

Sensitivity

39pg/mL

Sequence

50:

MPPYISAFQA ; AYIGIEVLIA ; LVSVPGNVLV ; IWAVKVNQAL ; RDATFCFIVS ;

100:

LAVADVAVGA ; LVIPLAILIN ; IGPQTYFHTC ; LMVACPVLIL ; TQSSILALLA ;

150:

IAVDRYLRVK ; IPLRYKTVVT ; QRRAAVAIAG ; CWILSLVVGL ; TPMFGWNNLS ;

200:

EVEQAWIANG ; SVGEPVIKCE ; FEKVISMEYM ; VYFNFFVWVL ; PPLLLMVLIY ;

250:

LEVFYLIRKQ ; LNKKVSASSG ; DPQKYYGKEL ; KIAKSLALIL ; FLFALSWLPL ;

300:

HILNCITLFC ; PTCQKPSILI ; YIAIFLTHGN ; SAMNPIVYAF ; RIHKFRVTFL ;

326:

KIWNDHFRCQ ; PKPPIEEDIP ; EEKADD

Sequence Length

326

String

Q60612

Uniprot	Q60612
Gene Name	Adora1
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:11539
Unigene	Mm.298908

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SKU	Supplier No.	Size	Your price	Quantity
BHE13800455	E12273m	96T	$776		Add to Cart

ELISA Kit for Mouse Adenosine receptor A1

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