ELISA Kit for Mouse 14-3-3 protein theta

Mfr.No.

E14293m

Alternative Names

14-3-3 protein tau

Categories

Elisa

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Specificity

Natural and recombinant Mouse 14-3-3 protein theta

Subcellular Location

Cytoplasm

Detection Range

0.78-50ng/mL

Entryname

1433T_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 14-3-3 protein theta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-106%	95-102%	90-110%	96-104%
EDTA plasma(n=5)	91-104%	93-103%	92-106%	93-103%
heparin plasma(n=5)	94-101%	91-107%	96-101%	92-105%

Method Type

Sandwich

Platform

PF00244

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.5%

Protein Name

14-3-3 protein theta

Recovery

Recovery was determined by spiking various levels of 14-3-3 protein theta into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	94-103
Plasma	98	92-101

Sensitivity

0.47ng/mL

Sequence

50:

MEKTELIQKA ; KLAEQAERYD ; DMATCMKAVT ; EQGAELSNEE ; RNLLSVAYKN ;

100:

VVGGRRSAWR ; VISSIEQKTD ; TSDKKLQLIK ; DYREKVESEL ; RSICTTVLEL ;

150:

LDKYLIANAT ; NPESKVFYLK ; MKGDYFRYLA ; EVACGDDRKQ ; TIENSQGAYQ ;

200:

EAFDISKKEM ; QPTHPIRLGL ; ALNFSVFYYE ; ILNNPELACT ; LAKTAFDEAI ;

245:

AELDTLNEDS ; YKDSTLIMQL ; LRDNLTLWTS ; DSAGEECDAA ; EGAEN

Sequence Length

245

String

P68254

Sub Unit

Homodimer (By similarity). Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with SSH1 (By similarity). Interacts with GAB2 (By similarity). Interacts with CDK16.

Uniprot	P68254
Gene Name	Ywhaq
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:100503129
Unigene	Mm.289630

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE13800088	E14293m	96T	$776		Add to Cart

ELISA Kit for Mouse 14-3-3 protein theta

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of ELISA Kit for Mouse 14-3-3 protein theta

Rate it now..!!