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ELISA Kit for Human V(D)J recombination-activating protein 2

SKU : BHE13809623

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E1551h
Categories	Elisa
Function	Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3.
Specificity	Natural and recombinant Human V(D)J recombination-activating protein 2
Subcellular Location	Nucleus
Detection Range	0.312-20ng/mL
Entryname	RAG2_HUMAN
Method Type	Sandwich
Platform	PF03089
Protein Name	V(D)J recombination-activating protein 2
Sensitivity	0.12ng/mL
Sequence	50: MSLQMVTVSN ; NIALIQPGFS ; LMNFDGQVFF ; FGQKGWPKRS ; CPTGVFHLDV ; 100: KHNHVKLKPT ; IFSKDSCYLP ; PLRYPATCTF ; KGSLESEKHQ ; YIIHGGKTPN ; 150: NEVSDKIYVM ; SIVCKNNKKV ; TFRCTEKDLV ; GDVPEARYGH ; SINVVYSRGK ; 200: SMGVLFGGRS ; YMPSTHRTTE ; KWNSVADCLP ; CVFLVDFEFG ; CATSYILPEL ; 250: QDGLSFHVSI ; AKNDTIYILG ; GHSLANNIRP ; ANLYRIRVDL ; PLGSPAVNCT ; 300: VLPGGISVSS ; AILTQTNNDE ; FVIVGGYQLE ; NQKRMICNII ; SLEDNKIEIR ; 350: EMETPDWTPD ; IKHSKIWFGS ; NMGNGTVFLG ; IPGDNKQVVS ; EGFYFYMLKC ; 400: AEDDTNEEQT ; TFTNSQTSTE ; DPGDSTPFED ; SEEFCFSAEA ; NSFDGDDEFD ; 450: TYNEDDEEDE ; SETGYWITCC ; PTCDVDINTW ; VPFYSTELNK ; PAMIYCSHGD ; 500: GHWVHAQCMD ; LAERTLIHLS ; AGSNKYYCNE ; HVEIARALHT ; PQRVLPLKKP ; 527: PMKSLRKKGS ; GKILTPAKKS ; FLRRLFD
Sequence Length	527
String	P55895
Sub Unit	Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2.

Uniprot	P55895
Gene Name	RAG2
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:5897
Unigene	Hs.714519

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SKU	Supplier No.	Size	Your price	Quantity
BHE13809623	E1551h	96T	$736		Add to Cart

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