JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Assay Kits / ELISA Kits

ELISA Kit for Human Splicing factor, proline- and glutamine-rich

SKU : BHE13809557

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

EIAAB SCIENCE INC, WUHAN

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	E11562h
Alternative Names	100 kDa DNA-pairing protein ; hPOMp100 ; DNA-binding p52/p100 complex ; 100 kDa subunit ; Polypyrimidine tract-binding protein-associated-splicing factor ; PSF ; PSF
Categories	Elisa
Function	DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
Specificity	Natural and recombinant Human Splicing factor, proline- and glutamine-rich
Subcellular Location	Nucleus matrix Predominantly in nuclear matrix.
Detection Range	0.156-10ng/mL
Entryname	SFPQ_HUMAN
Method Type	Sandwich
Platform	PF08075
Protein Name	Splicing factor, proline- and glutamine-rich
Sensitivity	0.08ng/mL
Sequence	50: MSRDRFRSRG ; GGGGGFHRRG ; GGGGRGGLHD ; FRSPPPGMGL ; NQNRGPMGPG ; 100: PGQSGPKPPI ; PPPPPHQQQQ ; QPPPQQPPPQ ; QPPPHQPPPH ; PQPHQQQQPP ; 150: PPPQDSSKPV ; VAQGPGPAPG ; VGSAPPASSS ; APPATPPTSG ; APPGSGPGPT ; 200: PTPPPAVTSA ; PPGAPPPTPP ; SSGVPTTPPQ ; AGGPPPPPAA ; VPGPGPGPKQ ; 250: GPGPGGPKGG ; KMPGGPKPGG ; GPGLSTPGGH ; PKPPHRGGGE ; PRGGRQHHPP ; 300: YHQQHHQGPP ; PGGPGGRSEE ; KISDSEGFKA ; NLSLLRRPGE ; KTYTQRCRLF ; 350: VGNLPADITE ; DEFKRLFAKY ; GEPGEVFINK ; GKGFGFIKLE ; SRALAEIAKA ; 400: ELDDTPMRGR ; QLRVRFATHA ; AALSVRNLSP ; YVSNELLEEA ; FSQFGPIERA ; 450: VVIVDDRGRS ; TGKGIVEFAS ; KPAARKAFER ; CSEGVFLLTT ; TPRPVIVEPL ; 500: EQLDDEDGLP ; EKLAQKNPMY ; QKERETPPRF ; AQHGTFEYEY ; SQRWKSLDEM ; 550: EKQQREQVEK ; NMKDAKDKLE ; SEMEDAYHEH ; QANLLRQDLM ; RRQEELRRME ; 600: ELHNQEMQKR ; KEMQLRQEEE ; RRRREEEMMI ; RQREMEEQMR ; RQREESYSRM ; 650: GYMDPRERDM ; RMGGGGAMNM ; GDPYGSGGQK ; FPPLGGGGGI ; GYEANPGVPP ; 700: ATMSGSMMGS ; DMRTERFGQG ; GAGPVGGQGP ; RGMGPGTPAG ; YGRGREEYEG ; 707: PNKKPRF
Sequence Length	707
String	P23246
Sub Unit	Interacts with PSPC1 (By similarity). Monomer and component of the SFPQ-NONO complex, which is probably a heterotetramer of two 52 kDa (NONO) and two 100 kDa (SFPQ) subunits. SFPQ is a component of spliceosome and U5.4/6 snRNP complexes. Interacts with SNRPA/U1A. Component of a snRNP-free complex with SNRPA/U1A. Part of complex consisting of SFPQ, NONO and MATR3. Interacts with polypyrimidine tract-binding protein 1/PTB. Part of a complex consisting of SFPQ, NONO and NR5A1. Interacts with RXRA, probably THRA, and SIN3A. Interacts with TOP1. Part of a complex consisting of SFPQ, NONO and TOP1. Interacts with SNRNP70 in apoptotic cells (By similarity). Interacts with RNF43. Interacts with PITX3 and NR4A2/NURR1.

Uniprot	P23246
Gene Name	SFPQ
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:6421
Unigene	Hs.355934

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13809557	E11562h	96T	$736		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.