ELISA Kit for Human Retinoic acid receptor beta

Mfr.No.

E10642h

Alternative Names

HBV-activated protein ; Nuclear receptor subfamily 1 group B member 2 ; RAR-epsilon ; HAP ; NR1B2

Categories

Elisa

Function

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.

Specificity

Natural and recombinant Human Retinoic acid receptor beta

Subcellular Location

Isoform Beta-4 Cytoplasm

Detection Range

0.156-10ng/mL

Entryname

RARB_HUMAN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RARB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-102%	92-101%	91-99%	93-100%
EDTA plasma(n=5)	94-103%	91-105%	92-106%	94-102%
heparin plasma(n=5)	93-105%	90-107%	95-101%	91-107%

Method Type

Sandwich

Platform

PF00104

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.2%

Protein Name

Retinoic acid receptor beta

Recovery

Recovery was determined by spiking various levels of RARB into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	92-103
Plasma	98	93-105

Sensitivity

0.089ng/mL

Sequence

50:

MTTSGHACPV ; PAVNGHMTHY ; PATPYPLLFP ; PVIGGLSLPP ; LHGLHGHPPP ;

100:

SGCSTPSPAT ; IETQSTSSEE ; LVPSPPSPLP ; PPRVYKPCFV ; CQDKSSGYHY ;

150:

GVSACEGCKG ; FFRRSIQKNM ; IYTCHRDKNC ; VINKVTRNRC ; QYCRLQKCFE ;

200:

VGMSKESVRN ; DRNKKKKETS ; KQECTESYEM ; TAELDDLTEK ; IRKAHQETFP ;

250:

SLCQLGKYTT ; NSSADHRVRL ; DLGLWDKFSE ; LATKCIIKIV ; EFAKRLPGFT ;

300:

GLTIADQITL ; LKAACLDILI ; LRICTRYTPE ; QDTMTFSDGL ; TLNRTQMHNA ;

350:

GFGPLTDLVF ; TFANQLLPLE ; MDDTETGLLS ; AICLICGDRQ ; DLEEPTKVDK ;

400:

LQEPLLEALK ; IYIRKRRPSK ; PHMFPKILMK ; ITDLRSISAK ; GAERVITLKM ;

450:

EIPGSMPPLI ; QEMLENSEGH ; EPLTPSSSGN ; TAEHSPSISP ; SSVENSGVSQ ;

455:

SPLVQ

Sequence Length

455

String

P10826

Sub Unit

Homodimer (By similarity). Heterodimer; with a RXR molecule. Binds DNA preferentially as a RAR/RXR heterodimer (By similarity). Interacts weakly with NCOR2.

Uniprot	P10826
Gene Name	RARB
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:5915
Unigene	Hs.654490

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SKU	Supplier No.	Size	Your price	Quantity
BHE13810223	E10642h	96T	$736		Add to Cart

ELISA Kit for Human Retinoic acid receptor beta

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