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ELISA Kit for Human Protein argonaute-2

SKU : BHE13800589

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E11449h
Alternative Names	Argonaute RISC catalytic component 2 ; Eukaryotic translation initiation factor 2C 2 ; eIF-2C 2 ; PAZ Piwi domain protein ; PPD ; Protein slicer ; EIF2C2
Categories	Elisa
Function	Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Specificity	Natural and recombinant Human Protein argonaute-2
Subcellular Location	Cytoplasm P-body Nucleus Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.
Detection Range	31.2-2000pg/ml
Entryname	AGO2_HUMAN
Method Type	Sandwich
Platform	PF08699
Protein Name	Protein argonaute-2
Sensitivity	15.66pg/mL
Sequence	50: MYSGAGPALA ; PPAPPPPIQG ; YAFKPPPRPD ; FGTSGRTIKL ; QANFFEMDIP ; 100: KIDIYHYELD ; IKPEKCPRRV ; NREIVEHMVQ ; HFKTQIFGDR ; KPVFDGRKNL ; 150: YTAMPLPIGR ; DKVELEVTLP ; GEGKDRIFKV ; SIKWVSCVSL ; QALHDALSGR ; 200: LPSVPFETIQ ; ALDVVMRHLP ; SMRYTPVGRS ; FFTASEGCSN ; PLGGGREVWF ; 250: GFHQSVRPSL ; WKMMLNIDVS ; ATAFYKAQPV ; IEFVCEVLDF ; KSIEEQQKPL ; 300: TDSQRVKFTK ; EIKGLKVEIT ; HCGQMKRKYR ; VCNVTRRPAS ; HQTFPLQQES ; 350: GQTVECTVAQ ; YFKDRHKLVL ; RYPHLPCLQV ; GQEQKHTYLP ; LEVCNIVAGQ ; 400: RCIKKLTDNQ ; TSTMIRATAR ; SAPDRQEEIS ; KLMRSASFNT ; DPYVREFGIM ; 450: VKDEMTDVTG ; RVLQPPSILY ; GGRNKAIATP ; VQGVWDMRNK ; QFHTGIEIKV ; 500: WAIACFAPQR ; QCTEVHLKSF ; TEQLRKISRD ; AGMPIQGQPC ; FCKYAQGADS ; 550: VEPMFRHLKN ; TYAGLQLVVV ; ILPGKTPVYA ; EVKRVGDTVL ; GMATQCVQMK ; 600: NVQRTTPQTL ; SNLCLKINVK ; LGGVNNILLP ; QGRPPVFQQP ; VIFLGADVTH ; 650: PPAGDGKKPS ; IAAVVGSMDA ; HPNRYCATVR ; VQQHRQEIIQ ; DLAAMVRELL ; 700: IQFYKSTRFK ; PTRIIFYRDG ; VSEGQFQQVL ; HHELLAIREA ; CIKLEKDYQP ; 750: GITFIVVQKR ; HHTRLFCTDK ; NERVGKSGNI ; PAGTTVDTKI ; THPTEFDFYL ; 800: CSHAGIQGTS ; RPSHYHVLWD ; DNRFSSDELQ ; ILTYQLCHTY ; VRCTRSVSIP ; 850: APAYYAHLVA ; FRARYHLVDK ; EHDSAEGSHT ; SGQSNGRDHQ ; ALAKAVQVHQ ; 859: DTLRTMYFA
Sequence Length	859
String	Q9UKV8
Sub Unit	Interacts with DICER1 through its Piwi domain and with TARBP2 during assembly of the RNA-induced silencing complex (RISC). Together, DICER1, EIF2C2/AGO2 and TARBP2 constitute the trimeric RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC). Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Note however that the term RISC has also been used to describe the trimeric RLC/miRLC. The formation of RISC complexes containing siRNAs rather than miRNAs appears to occur independently of DICER1. Interacts with EIF2C1/AGO1. Also interacts with DDB1, DDX5, DDX6, DDX20, DHX30, DHX36, DDX47, DHX9, EIF6, ELAVL, FXR1, GEMIN4, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, P4HA1, P4HB, RBM4, SART3, TNRC6A, TNRC6B, UPF1 and YBX1. Interacts with the P-body components DCP1A and XRN1. Associates with polysomes and messenger ribonucleoproteins (mNRPs). Interacts with RBM4; the interaction is modulated under stress-induced conditions, occurs under both cell proliferation and differentiation conditions and in a RNA-and phosphorylation-independent manner.

Uniprot	Q9UKV8
Gene Name	AGO2
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:27161
Unigene	Hs.743313

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SKU	Supplier No.	Size	Your price	Quantity
BHE13800589	E11449h	96T	$736		Add to Cart

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