ELISA Kit for Human Programmed cell death 1 ligand 1

Mfr.No.

E0513h

Alternative Names

B7 homolog 1 ; B7-H1 ; CD274 ; B7H1 ; PDCD1L1 ; PDCD1LG1 ; PDL1

Categories

Elisa

Function

Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Specificity

Natural and recombinant Human Programmed cell death 1 ligand 1

Subcellular Location

Isoform 2 Endomembrane system Single-pass type I membrane protein

Detection Range

0.312-20ng/mL

Entryname

PD1L1_HUMAN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PD1L1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	98-110%	97-109%	100-106%	98-108%
EDTA plasma(n=5)	95-109%	97-108%	99-106%	95-107%
heparin plasma(n=5)	96-106%	94-109%	98-107%	97-106%

Method Type

Sandwich

Platform

PF08205

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

Protein Name

Programmed cell death 1 ligand 1

Recovery

Recovery was determined by spiking various levels of PD1L1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	104	95-106
Plasma	102	93-105

Sensitivity

0.098ng/mL

Sequence

50:

MRIFAVFIFM ; TYWHLLNAFT ; VTVPKDLYVV ; EYGSNMTIEC ; KFPVEKQLDL ;

100:

AALIVYWEME ; DKNIIQFVHG ; EEDLKVQHSS ; YRQRARLLKD ; QLSLGNAALQ ;

150:

ITDVKLQDAG ; VYRCMISYGG ; ADYKRITVKV ; NAPYNKINQR ; ILVVDPVTSE ;

200:

HELTCQAEGY ; PKAEVIWTSS ; DHQVLSGKTT ; TTNSKREEKL ; FNVTSTLRIN ;

250:

TTTNEIFYCT ; FRRLDPEENH ; TAELVIPELP ; LAHPPNERTH ; LVILGAILLC ;

290:

LGVALTFIFR ; LRKGRMMDVK ; KCGIQDTNSK ; KQSDTHLEET ;

Sequence Length

290

String

Q9NZQ7

Sub Unit

Interacts with PDCD1.

Uniprot	Q9NZQ7
Gene Name	CD274
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:29126
Unigene	Hs.521989

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SKU	Supplier No.	Size	Your price	Quantity
BHE13808765	E0513h	96T	$736		Add to Cart

ELISA Kit for Human Programmed cell death 1 ligand 1

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