ELISA Kit for Human NAD-dependent deacetylase sirtuin-2

Mfr.No.

E0430h

Alternative Names

SIR2-like protein 2 ; SIR2L ; SIR2L2

Categories

Elisa

Function

NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.

Specificity

Natural and recombinant Human NAD-dependent deacetylase sirtuin-2

Subcellular Location

Cytoplasm cytoskeleton Colocalizes with microtubules.

Detection Range

0.312-20ng/mL

Entryname

SIRT2_HUMAN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIRT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	91-107%	93-104%	92-104%	95-103%
EDTA plasma(n=5)	92-105%	94-105%	93-105%	93-106%
heparin plasma(n=5)	95-102%	96-102%	94-102%	96-101%

Method Type

Sandwich

Platform

PF02146

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.9%

Protein Name

NAD-dependent deacetylase sirtuin-2

Recovery

Recovery was determined by spiking various levels of SIRT2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	95-103
Plasma	98	96-101

Sensitivity

0.11ng/mL

Sequence

50:

MAEPDPSHPL ; ETQAGKVQEA ; QDSDSDSEGG ; AAGGEADMDF ; LRNLFSQTLS ;

100:

LGSQKERLLD ; ELTLEGVARY ; MQSERCRRVI ; CLVGAGISTS ; AGIPDFRSPS ;

150:

TGLYDNLEKY ; HLPYPEAIFE ; ISYFKKHPEP ; FFALAKELYP ; GQFKPTICHY ;

200:

FMRLLKDKGL ; LLRCYTQNID ; TLERIAGLEQ ; EDLVEAHGTF ; YTSHCVSASC ;

250:

RHEYPLSWMK ; EKIFSEVTPK ; CEDCQSLVKP ; DIVFFGESLP ; ARFFSCMQSD ;

300:

FLKVDLLLVM ; GTSLQVQPFA ; SLISKAPLST ; PRLLINKEKA ; GQSDPFLGMI ;

350:

MGLGGGMDFD ; SKKAYRDVAW ; LGECDQGCLA ; LAELLGWKKE ; LEDLVRREHA ;

389:

SIDAQSGAGV ; PNPSTSASPK ; KSPPPAKDEA ; RTTEREKPQ

Sequence Length

389

String

Q8IXJ6

Sub Unit

Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton.

Uniprot	Q8IXJ6
Gene Name	SIRT2
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:22933
Unigene	Hs.466693

Reference

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SKU	Supplier No.	Size	Your price	Quantity
BHE13810502	E0430h	96T	$736		Add to Cart

ELISA Kit for Human NAD-dependent deacetylase sirtuin-2

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