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ELISA Kit for Human Mucin-5AC

SKU : BHE13807920

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E0756h
Alternative Names	Gastric mucin ; Lewis B blood group antigen ; LeB ; Major airway glycoprotein ; Mucin-5 subtype AC ; tracheobronchial ; Tracheobronchial mucin ; TBM ; MUC5
Categories	Elisa
Function	Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particules that are subsequently removed by the mucocilary system.
Specificity	Natural and recombinant Human Mucin-5AC
Subcellular Location	Secreted
Detection Range	0.78-50ng/mL
Entryname	MUC5A_HUMAN
Method Type	Sandwich
Platform	PF08742
Protein Name	Mucin-5AC
Sensitivity	0.195ng/mL
Sequence	50: MSVGRRKLAL ; LWALALALAC ; TRHTGHAQDG ; SSESSYKHHP ; ALSPIARGPS ; 100: GVPLRGATVF ; PSLRTIPVVR ; ASNPAHNGRV ; CSTWGSFHYK ; TFDGDVFRFP ; 150: GLCNYVFSEH ; CGAAYEDFNI ; QLRRSQESAA ; PTLSRVLMKV ; DGVVIQLTKG ; 200: SVLVNGHPVL ; LPFSQSGVLI ; QQSSSYTKVE ; ARLGLVLMWN ; HDDSLLLELD ; 250: TKYANKTCGL ; CGDFNGMPVV ; SELLSHNTKL ; TPMEFGNLQK ; MDDPTEQCQD ; 300: PVPEPPRNCS ; TGFGICEELL ; HGQLFSGCVA ; LVDVGSYLEA ; CRQDLCFCED ; 350: TDLLSCVCHT ; LAEYSRQCTH ; AGGLPQDWRG ; PDFCPQKCPN ; NMQYHECRSP ; 400: CADTCSNQEH ; SRACEDHCVA ; GCFCPEGTVL ; DDIGQTGCVP ; VSKCACVYNG ; 450: AAYAPGATYS ; TDCTNCTCSG ; GRWSCQEVPC ; PDTCSVLGGA ; HFSTFDGKQY ; 500: TVHGDCSYVL ; TKPCDSSAFT ; VLAELRRCGL ; TDSETCLKSV ; TLSLDGAQTV ; 550: VVIKASGEVF ; LNQIYTQLPI ; SAANVTIFRP ; STFFIIAQTS ; LGLQLNLQPV ; 600: PTMQLFMQLA ; PKLRGQTCGL ; CGNFNSIQAD ; DFRTLSGVVE ; ATAAAFFNTF ; 650: KTQAACPNIR ; NSFEDPCSLS ; VENEKYAQHW ; CSQLTDADGP ; FGRCHAAVKP ; 700: GTYYSNCVFD ; TCNCERSEDC ; LCAALSSYVH ; ACAAKGVQLG ; GWRDGVCTKP ; 750: MTTCPKSMTY ; HYHVSTCQPT ; CRSLSEGDIT ; CSVGFIPVDG ; CICPKGTFLD ; 800: DTGKCVQASN ; CPCYHRGSMI ; PNGESVHDSG ; AICTCTHGKL ; SCIGGQAPAP ; 850: VCAAPMVFFD ; CRNATPGDTG ; AGCQKSCHTL ; DMTCYSPQCV ; PGCVCPDGLV ; 900: ADGEGGCITA ; EDCPCVHNEA ; SYRAGQTIRV ; GCNTCTCDSR ; MWRCTDDPCL ; 950: ATCAVYGDGH ; YLTFDGQSYS ; FNGDCEYTLV ; QNHCGGKDST ; QDSFRVVTEN ; 1000: VPCGTTGTTC ; SKAIKIFLGG ; FELKLSHGKV ; EVIGTDESQE ; VPYTIQQMGI ; 1050: YLVVDTDIGL ; VLLWDKKTSI ; FINLSPEFKG ; RVCGLCGNFD ; DIAVNDFATR ; 1100: SRSVVGDVLE ; FGNSWKLSPS ; CPDALAPKDP ; CTANPFRKSW ; AQKQCSILHG ; 1150: PTFAACHAHV ; EPARYYEACV ; NDACACDSGG ; DCECFCTAVA ; AYAQACHEVG ; 1200: LCVCLRTPSI ; CPLFCDYYNP ; EGQCEWHYQP ; CGVPCLRTCR ; NPRGDCLRDV ; 1250: RGLEGCYPKC ; PPEAPIFDED ; KMQCVATCPT ; PPLPPRCHVH ; GKSYRPGAVV ; 1300: PSDKNCQSCL ; CTERGVECTY ; KAEACVCTYN ; GQRFHPGDVI ; YHTTDGTGGC ; 1350: ISARCGANGT ; IERRVYPCSP ; TTPVPPTTFS ; FSTPPLVVSS ; THTPSNGPSS ; 1400: AHTGPPSSAW ; PTTAGTSPRT ; RLPTASASLP ; PVCGEKCLWS ; PWMDVSRPGR ; 1450: GTDSGDFDTL ; ENLRAHGYRV ; CESPRSVECR ; AEDAPGVPLR ; ALGQRVQCSP ; 1500: DVGLTCRNRE ; QASGLCYNYQ ; IRVQCCTPLP ; CSTSSSPAQT ; TPPTTSKTTE ; 1550: TRASGSSAPS ; STPGTVSLST ; ARTTPAPGTA ; TSVKKTFSTP ; SPPPVPATST ; 1600: SSMSTTAPGT ; SVVSSKPTPT ; EPSTSSCLQE ; LCTWTEWIDG ; SYPAPGINGG ; 1650: DFDTFQNLRD ; EGYTFCESPR ; SVQCRAESFP ; NTPLADLGQD ; VICSHTEGLI ; 1700: CLNKNQLPPI ; CYNYEIRIQC ; CETVNVCRDI ; TRLPKTVATT ; RPTPHPTGAQ ; 1750: TQTTFTTHMP ; SASTEQPTAT ; SRGGPTATSV ; TQGTHTTLVT ; RNCHPRCTWT ; 1800: KWFDVDFPSP ; GPHGGDKETY ; NNIIRSGEKI ; CRRPEEITRV ; QCRAKSHPEV ; 1850: SIEHLGQVVQ ; CSREEGLVCR ; NQDQQGPFKM ; CLNYEVRVLC ; CETPRGCHMT ; 1900: STPGSTSSSP ; AQTTPSTTSK ; TTEIQASGSS ; APSSTPGTVS ; LSTARTTPAP ; 1950: GTATSVKKTF ; STPSPPPVPA ; TSTSSMSTTA ; PGTSVVSSKP ; TPTEPSTSSC ; 2000: LQELCTWTEW ; IDGSYPAPGI ; NGGDFDTFQN ; LRDEGYTFCE ; SPRSVQCRAE ; 2050: SFPNTPLGRL ; GQDVICSHTE ; GLICLNKNQL ; PPICYNYEIR ; IQCCETVNVC ; 2100: RDITRPPKTV ; ATTRPTPHPT ; GAQTQTTFTT ; HMPSASTEQP ; TATSRGGPTA ; 2150: TSVTQGTHTT ; PVTRNCHPRC ; TWTTWFDVDF ; PSPGPHGGDK ; ETYNNIIRSG ; 2200: EKICRRPEEI ; TRLQCRAKSH ; PEVSIEHLGQ ; VVQCSREEGL ; VCRNQDQQGP ; 2250: FKMCLNIEVR ; VLCCETPKGC ; PVTSTPVTAP ; STPSGRAISP ; TQSTSSWQKS ; 2300: RTTTLVTTST ; TSTPQTSTTY ; AHTTSTTSAP ; TARTTSAPTT ; STTSVPTTST ; 2350: ISGPKTTPSP ; VPTTSTTSAA ; TTSTISAPTT ; STTSVPGTTP ; SPVLTTSTTS ; 2400: APTTRTTSAS ; PAGTTSGPGN ; TPSPVPTTST ; ISAPTTSITS ; APTTSTTSAP ; 2450: TSSTTSGPGT ; TPSPVPTTSI ; TSAPTTSTTS ; APTTSTTSAP ; TTSTTSAPTT ; 2500: STTSAPTTST ; TSTPTSSTTS ; TPQTSTTSAS ; TTSITSGPGT ; TPSPVPTTST ; 2550: TSAPTTSTTS ; AATTSTISAP ; TTSTTSAPTT ; STTSASTASK ; TSGLGTTPSP ; 2600: IPTTSTTSPP ; TTSTTSASTA ; SKTSGPGTTP ; SPVPTTSTIF ; APRTSTTSAS ; 2650: TTSTTPGPGT ; TPSPVPTTST ; ASVSKTSTSH ; VSISKTTHSQ ; PVTRDCHLRC ; 2700: TWTKWFDVDF ; PSPGPHGGDK ; ETYNNIIRSG ; EKICRRPEEI ; TRLQCRAESH ; 2750: PEVSIEHLGQ ; VVQCSREEGL ; VCRNQDQQGP ; FKMCLNYEVR ; VLCCETPKGC ; 2800: PVTSTPVTAP ; STPSGRATSP ; TQSTSSWQKS ; RTTTLVTTST ; TSTPQTSTTS ; 2850: APTTSTTSAP ; TTSTTSAPTT ; STTSTPQTSI ; SSAPTSSTTS ; APTSSTISAR ; 2900: TTSIISAPTT ; STTSSPTTST ; TSATTTSTTS ; APTSSTTSTP ; QTSKTSAATS ; 2950: STTSSSGTTP ; SPVTTTSTAS ; VSKTSTSHVS ; VSKTTHSQPV ; TRDCHPRCTW ; 3000: TKWFDVDFPS ; PGPHGGDKET ; YNNIIRSGEK ; ICRRPQEITR ; LQCRAKSHPE ; 3050: VSIEHLGQVV ; QCSREEGLVC ; RNQDQQGPFK ; MCLNYEVRVL ; CCETPKGCPV ; 3100: TSTSVTAPSP ; LVGEPPAQTQ ; STSSWQKSRT ; TTLVTSSITS ; TTQTSTTSAP ; 3150: TTSTTPASIP ; STTSAPTTST ; TSAPTTSTTS ; APTTSTTSTP ; QTTTSSAPTS ; 3200: STTSAPTTST ; ISAPTTSTIS ; APTTSTTSAP ; TASTTSAPTS ; TSSAPTTNTT ; 3250: SAPTTSTTSA ; PITSTISAPT ; TSTTSTPQTS ; TISSPTTSTT ; PTPQTSTTSS ; 3300: PTTSTTSAPT ; TSTTSAPTTS ; TTSTPQTSIS ; SAPTSSTTSA ; PTASTISAPT ; 3350: TSTTSFHTTS ; TTSPPTSSTS ; STPQTSKTSA ; ATSSTTSGSG ; TTPSPVPTTS ; 3400: TASVSKTSTS ; HVSVSKTTHS ; QPVTRDCHPR ; CTWTKWFDVD ; FPSPGPHGGD ; 3450: KETYNNIIRS ; GEKICRRPEE ; ITRLQCRAES ; HPEVSIEHLG ; QVVQCSREEG ; 3500: LVCRNQDQQG ; PFKMCLNYEV ; RVLCCETPKG ; CPVTSTPVTA ; PSTPSGRATS ; 3550: PTQSTSSWQK ; SRTTTLVTTS ; TTSTPQTSTT ; SAPTTSTIPA ; STPSTTSAPT ; 3600: TSTTSAPTTS ; TTSAPTHRTT ; SGPTTSTTLA ; PTTSTTSAPT ; TSTNSAPTTS ; 3650: TISASTTSTI ; SAPTTSTISS ; PTSSTTSTPQ ; TSKTSAATSS ; TTSGSGTTPS ; 3700: PVPTTSTTSA ; STTSTTSAPT ; TSTTSGPGTT ; PSPVPSTSIT ; SAATTSTTSA ; 3750: PTTRTTSAPT ; SSMTSGPGTT ; PSPVPTTSTT ; SAPTTSTTSG ; PGTTPSPVPT ; 3800: TSTTSAPITS ; TTSGPGSTPS ; PVPTTSTTSA ; PTTSTTSAST ; ASTTSGPTTS ; 3850: TTSASTTSTI ; SPLTTSTTSA ; PITSMPSGPG ; TTPSPVPTTS ; TTSAPTTSTT ; 3900: SGPGTTPSPV ; PTTSTTSAPT ; TSTTSASTAS ; TTSGPGTTPS ; PVPTTSTTSA ; 3950: PTTSTTSAST ; ASTTSGPGTS ; LSPVPTTSTT ; SAPTTSTTSG ; PGTTPSPVPT ; 4000: TSTTSAPTTS ; TTSGPGTTPS ; PVPTTSTTPV ; SKTSTSHLSV ; SKTTHSQPVT ; 4050: SDCHPLCAWT ; KWFDVDFPSP ; GPHGGDKETY ; NNIIRSGEKI ; CRRPEEITRL ; 4100: QCRAESHPEV ; NIEHLGQVVQ ; CSREEGLVCR ; NQDQQGPFKM ; CLNYEVRVLC ; 4150: CETPRGCPVT ; SVTPYGTSPT ; NALYPSLSTS ; MVSASVASTS ; VASSSVASSS ; 4200: VAYSTQTCFC ; NVADRLYPAG ; STIYRHRDLA ; GHCYYALCSQ ; DCQVVRGVDS ; 4250: DCPSTTLPPA ; PATSPSISTS ; EPVTELGCPN ; AVPPRKKGET ; WATPNCSEAT ; 4300: CEGNNVISLS ; PRTCPRVEKP ; TCANGYPAVK ; VADQDGCCHH ; YQCQCVCSGW ; 4350: GDPHYITFDG ; TYYTFLDNCT ; YVLVQQIVPV ; YGHFRVLVDN ; YFCGAEDGLS ; 4400: CPRSIILEYH ; QDRVVLTRKP ; VHGVMTNEII ; FNNKVVSPGF ; RKNGIVVSRI ; 4450: GVKMYATIPE ; LGVQVMFSGL ; IFSVEVPFSK ; FANNTEGQCG ; TCTNDRKDEC ; 4500: RTPRGTVVAS ; CSEMSGLWNV ; SIPDQPACHR ; PHPTPTTVGP ; TTVGSTTVGP ; 4550: TTVGSTTVGP ; TTPPAPCLPS ; PICHLILSKV ; FEPCHTVIPP ; LLFYEGCVFD ; 4600: RCHMTDLDVV ; CSSLELYAAL ; CASHDICIDW ; RGRTGHMCPF ; TCPADKVYQP ; 4650: CGPSNPSYCY ; GNDSASLGAL ; REAGPITEGC ; FCPEGMTLFS ; TSAQVCVPTG ; 4700: CPRCLGPHGE ; PVKVGHTVGM ; DCQECTCEAA ; TWTLTCRPKL ; CPLPPACPLP ; 4750: GFVPVPAAPQ ; AGQCCPQYSC ; ACNTSRCPAP ; VGCPEGARAI ; PTYQEGACCP ; 4800: VQNCSWTVCS ; INGTLYQPGA ; VVSSSLCETC ; RCELPGGPPS ; DAFVVSCETQ ; 4850: ICNTHCPVGF ; EYQEQSGQCC ; GTCVQVACVT ; NTSKSPAHLF ; YPGETWSDAG ; 4900: NHCVTHQCEK ; HQDGLVVVTT ; KKACPPLSCS ; LDEARMSKDG ; CCRFCPLPPP ; 4950: PYQNQSTCAV ; YHRSLIIQQQ ; GCSSSEPVRL ; AYCRGNCGDS ; SSMYSLEGNT ; 5000: VEHRCQCCQE ; LRTSLRNVTL ; HCTDGSSRAF ; SYTEVEECGC ; MGRRCPAPGD ; 5030: TQHSEEAEPE ; PSQEAESGSW ; ERGVPVSPMH ;
Sequence Length	5030
Sub Unit	Multimeric. Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD).

Uniprot	P98088
Gene Name	MUC5AC
Immunogen Species	Homo Sapiens (Human)
Unigene	Hs.534332

Reference	1. "Human mucin gene MUC5AC: organization of its 5'-region and central repetitive region." Escande F., Aubert J.-P., Porchet N., Buisine M.P. Biochem. J.358:763-772(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-2448;NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2449-3797;NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3798-4169 2. "Cloning of the amino-terminal and 5'-flanking region of the human MUC5AC mucin gene and transcriptional up-regulation by bacterial exoproducts." Li D., Gallup M., Fan N., Szymkowski D.E., Basbaum C.B. J. Biol. Chem.273:6812-6820(1998) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-1104 tissue: Trachea. 3. "Cloning and analysis of human gastric mucin cDNA reveals two types of conserved cysteine-rich domains." Klomp L.W., Van Rens L., Strous G.J. Biochem. J.308:831-838(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1005-1854 4. "Proteolytic fragmentation and peptide mapping of human carboxyamidomethylated tracheobronchial mucin." Rose M.C., Kaufman B., Martin B.M. J. Biol. Chem.264:8193-8199(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 1752-1773; 1796-1805; 2125-2146; 2169-2178; 2655-2676; 2697-2708; 2953-2974; 2997-3006; 3386-3407; 3428-3439 AND 4012-4033 tissue: Tracheobronchial mucosa. 5. "Genomic organization of the 3'-region of the human MUC5AC mucin gene: additional evidence for a common ancestral gene for the 11p15.5 mucin gene family." Buisine M.P., Desseyn J.-L., Porchet N., Degand P., Laine A., Aubert J.-P. Biochem. J.332:729-738(1998) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 3950-5030;VARIANT PRO-4897 tissue: Placenta. tissue: Trachea. 6. "Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5)." Meerzaman D., Charles P., Daskal E., Polymeropoulos M.H., Martin B.M., Rose M.C. J. Biol. Chem.269:12932-12939(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3990-5030;PARTIAL PROTEIN SEQUENCE;TISSUE SPECIFICITY tissue: Nasal polyp. 7. "Characterization of a mucin cDNA clone isolated from HT-29 mucus secreting cells: the 3' end of MUC5AC?" Lesuffleur T., Roche F., Hill A.S., Lacasa M., Fox M., Swallow D.M., Zweibaum A., Real F.X. J. Biol. Chem.270:13665-13673(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4081-5030 8. "Cleavage in the GDPH sequence of the C-terminal cysteine-rich part of the human MUC5AC mucin." Lidell M.E., Hansson G.C. Biochem. J.399:121-129(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 4303-4312;PROTEOLYTIC PROCESSING;MUTAGENESIS OF ASP-4302 9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4457-5030;VARIANT PRO-4897 tissue: Colon. 10. "The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.more... BMC Genomics8:399-399(2007) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4569-5030 tissue: Stomach. 11. "In vivo glycosylation of mucin tandem repeats." Silverman H.S., Parry S., Sutton-Smith M., Burdick M.D., McDermott K., Reid C.J., Batra S.K., Morris H.R., Hollingsworth M.A., Dell A., Harris A. Glycobiology11:459-471(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE OF O-LINKED CARBOHYDRATES 12. "The MUC5AC glycoprotein is the primary receptor for Helicobacter pylori in the human stomach." Van de Bovenkamp J.H., Mahdavi J., Korteland-Van Male A.M., Bueller H.A., Einerhand A.W., Boren T., Dekker J. Helicobacter8:521-532(2003) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION AS LEWIS B BLOOD GROUP ANTIGEN;TISSUE SPECIFICITY;INTERACTION WITH HELICOBACTER PYLORI 13. "C-Mannosylation of MUC5AC and MUC5B Cys subdomains." Perez-Vilar J., Randell S.H., Boucher R.C. Glycobiology14:325-337(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT TRP-2122;SUBCELLULAR LOCATION;MUTAGENESIS OF TRP-2122

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