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ELISA Kit for Human E3 ubiquitin-protein ligase UBR4

SKU : BHE13811822

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E0192h
Alternative Names	600 kDa retinoblastoma protein-associated factor ; N-recognin-4 ; RING-type E3 ubiquitin transferase UBR4 ; Retinoblastoma-associated factor of 600 kDa ; RBAF600 ; Zinc finger UBR1-type protein 1 ; KIAA0462 ; KIAA1307 ; RBAF600 ; ZUBR1
Categories	Elisa
Function	E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions.
Specificity	Natural and recombinant Human E3 ubiquitin-protein ligase UBR4
Subcellular Location	Membrane Multi-pass membrane protein Cytoplasm Cytoplasm cytoskeleton Nucleus Concentrates at the leading edge of membrane structures involved in actin motility.
Detection Range	0.625-40ng/mL
Entryname	UBR4_HUMAN
Method Type	Competitive
Platform	PF02207
Protein Name	E3 ubiquitin-protein ligase UBR4
Sensitivity	0.22ng/mL
Sequence	50: MATSGGEEAA ; AAAPAPGTPA ; TGADTTPGWE ; VAVRPLLSAS ; YSAFEMKELP ; 100: QLVASVIESE ; SEILHHEKQY ; EPFYSSFVAL ; STHYITTVCS ; LIPRNQLQSV ; 150: AAACKVLIEF ; SLLRLENPDE ; ACAVSQKHLI ; LLIKGLCTGC ; SRLDRTEIIT ; 200: FTAMMKSAKL ; PQTVKTLSDV ; EDQKELASPV ; SPELRQKEVQ ; MNFLNQLTSV ; 250: FNPRTVASQP ; ISTQTLVEGE ; NDEQSSTDQA ; SAIKTKNVFI ; AQNVASLQEL ; 300: GGSEKLLRVC ; LNLPYFLRYI ; NRFQDAVLAN ; SFFIMPATVA ; DATAVRNGFH ; 350: SLVIDVTMAL ; DTLSLPVLEP ; LNPSRLQDVT ; VLSLSCLYAG ; VSVATCMAIL ; 400: HVGSAQQVRT ; GSTSSKEDDY ; ESDAATIVQK ; CLEIYDMIGQ ; AISSSRRAGG ; 450: EHYQNFQLLG ; AWCLLNSLFL ; ILNLSPTALA ; DKGKEKDPLA ; ALRVRDILSR ; 500: TKEGVGSPKL ; GPGKGHQGFG ; VLSVILANHA ; IKLLTSLFQD ; LQVEALHKGW ; 550: ETDGPPAALS ; IMAQSTSIQR ; IQRLIDSVPL ; MNLLLTLLST ; SYRKACVLQR ; 600: QRKGSMSSDA ; SASTDSNTYY ; EDDFSSTEED ; SSQDDDSEPI ; LGQWFEETIS ; 650: PSKEKAAPPP ; PPPPPPLESS ; PRVKSPSKQA ; PGEKGNILAS ; RKDPELFLGL ; 700: ASNILNFITS ; SMLNSRNNFI ; RNYLSVSLSE ; HHMATLASII ; KEVDKDGLKG ; 750: SSDEEFAAAL ; YHFNHSLVTS ; DLQSPNLQNT ; LLQQLGVAPF ; SEGPWPLYIH ; 800: PQSLSVLSRL ; LLIWQHKASA ; QGDPDVPECL ; KVWDRFLSTM ; KQNALQGVVP ; 850: SETEDLNVEH ; LQMLLLIFHN ; FTETGRRAIL ; SLFVQIIQEL ; SVNMDAQMRF ; 900: VPLILARLLL ; IFDYLLHQYS ; KAPVYLFEQV ; QHNLLSPPFG ; WASGSQDSNS ; 950: RRATTPLYHG ; FKEVEENWSK ; HFSSDAVPHP ; RFYCVLSPEA ; SEDDLNRLDS ; 1000: VACDVLFSKL ; VKYDELYAAL ; TALLAAGSQL ; DTVRRKENKN ; VTALEACALQ ; 1050: YYFLILWRIL ; GILPPSKTYI ; NQLSMNSPEM ; SECDILHTLR ; WSSRLRISSY ; 1100: VNWIKDHLIK ; QGMKAEHASS ; LLELASTTKC ; SSVKYDVEIV ; EEYFARQISS ; 1150: FCSIDCTTIL ; QLHEIPSLQS ; IYTLDAAISK ; VQVSLDEHFS ; KMAAETDPHK ; 1200: SSEITKNLLP ; ATLQLIDTYA ; SFTRAYLLQN ; FNEEGTTEKP ; SKEKLQGFAA ; 1250: VLAIGSSRCK ; ANTLGPTLVQ ; NLPSSVQTVC ; ESWNNINTNE ; FPNIGSWRNA ; 1300: FANDTIPSES ; YISAVQAAHL ; GTLCSQSLPL ; AASLKHTLLS ; LVRLTGDLIV ; 1350: WSDEMNPPQV ; IRTLLPLLLE ; SSTESVAEIS ; SNSLERILGP ; AESDEFLARV ; 1400: YEKLITGCYN ; ILANHADPNS ; GLDESILEEC ; LQYLEKQLES ; SQARKAMEEF ; 1450: FSDSGELVQI ; MMATANENLS ; AKFCNRVLKF ; FTKLFQLTEK ; SPNPSLLHLC ; 1500: GSLAQLACVE ; PVRLQAWLTR ; MTTSPPKDSD ; QLDVIQENRQ ; LLQLLTTYIV ; 1550: RENSQVGEGV ; CAVLLGTLTP ; MATEMLANGD ; GTGFPELMVV ; MATLASAGQG ; 1600: AGHLQLHNAA ; VDWLSRCKKY ; LSQKNVVEKL ; NANVMHGKHV ; MILECTCHIM ; 1650: SYLADVTNAL ; SQSNGQGPSH ; LSVDGEERAI ; EVDSDWVEEL ; AVEEEDSQAE ; 1700: DSDEDSLCNK ; LCTFTITQKE ; FMNQHWYHCH ; TCKMVDGVGV ; CTVCAKVCHK ; 1750: DHEISYAKYG ; SFFCDCGAKE ; DGSCLALVKR ; TPSSGMSSTM ; KESAFQSEPR ; 1800: ISESLVRHAS ; TSSPADKAKV ; TISDGKVADE ; EKPKKSSLCR ; TVEGCREELQ ; 1850: NQANFSFAPL ; VLDMLNFLMD ; AIQTNFQQAS ; AVGSSSRAQQ ; ALSELHTVEK ; 1900: AVEMTDQLMV ; PTLGSQEGAF ; ENVRMNYSGD ; QGQTIRQLIS ; AHVLRRVAMC ; 1950: VLSSPHGRRQ ; HLAVSHEKGK ; ITVLQLSALL ; KQADSSKRKL ; TLTRLASAPV ; 2000: PFTVLSLTGN ; PCKEDYLAVC ; GLKDCHVLTF ; SSSGSVSDHL ; VLHPQLATGN ; 2050: FIIKAVWLPG ; SQTELAIVTA ; DFVKIYDLCV ; DALSPTFYFL ; LPSSKIRDVT ; 2100: FLFNEEGKNI ; IVIMSSAGYI ; YTQLMEEASS ; AQQGPFYVTN ; VLEINHEDLK ; 2150: DSNSQVAGGG ; VSVYYSHVLQ ; MLFFSYCQGK ; SFAATISRTT ; LEVLQLFPIN ; 2200: IKSSNGGSKT ; SPALCQWSEV ; MNHPGLVCCV ; QQTTGVPLVV ; MVKPDTFLIQ ; 2250: EIKTLPAKAK ; IQDMVAIRHT ; ACNEQQRTTM ; ILLCEDGSLR ; IYMANVENTS ; 2300: YWLQPSLQPS ; SVISIMKPVR ; KRKTATITTR ; TSSQVTFPID ; FFEHNQQLTD ; 2350: VEFGGNDLLQ ; VYNAQQIKHR ; LNSTGMYVAN ; TKPGGFTIEI ; SNNNSTMVMT ; 2400: GMRIQIGTQA ; IERAPSYIEI ; FGRTMQLNLS ; RSRWFDFPFT ; REEALQADKK ; 2450: LNLFIGASVD ; PAGVTMIDAV ; KIYGKTKEQF ; GWPDEPPEEF ; PSASVSNICP ; 2500: SNLNQSNGTG ; DSDSAAPTTT ; SGTVLERLVV ; SSLEALESCF ; AVGPIIEKER ; 2550: NKNAAQELAT ; LLLSLPAPAS ; VQQQSKSLLA ; SLHTSRSAYH ; SHKDQALLSK ; 2600: AVQCLNTSSK ; EGKDLDPEVF ; QRLVITARSI ; AIMRPNNLVH ; FTESKLPQME ; 2650: TEGMDEGKEP ; QKQLEGDCCS ; FITQLVNHFW ; KLHASKPKNA ; FLAPACLPGL ; 2700: THIEATVNAL ; VDIIHGYCTC ; ELDCINTASK ; IYMQMLLCPD ; PAVSFSCKQA ; 2750: LIRVLRPRNK ; RRHVTLPSSP ; RSNTPMGDKD ; DDDDDDADEK ; MQSSGIPNGG ; 2800: HIRQESQEQS ; EVDHGDFEMV ; SESMVLETAE ; NVNNGNPSPL ; EALLAGAEGF ; 2850: PPMLDIPPDA ; DDETMVELAI ; ALSLQQDQQG ; SSSSALGLQS ; LGLSGQAPSS ; 2900: SSLDAGTLSD ; TTASAPASDD ; EGSTAATDGS ; TLRTSPADHG ; GSVGSESGGS ; 2950: AVDSVAGEHS ; VSGRSSAYGD ; ATAEGHPAGP ; GSVSSSTGAI ; STTTGHQEGD ; 3000: GSEGEGEGET ; EGDVHTSNRL ; HMVRLMLLER ; LLQTLPQLRN ; VGGVRAIPYM ; 3050: QVILMLTTDL ; DGEDEKDKGA ; LDNLLSQLIA ; ELGMDKKDVS ; KKNERSALNE ; 3100: VHLVVMRLLS ; VFMSRTKSGS ; KSSICESSSL ; ISSATAAALL ; SSGAVDYCLH ; 3150: VLKSLLEYWK ; SQQNDEEPVA ; TSQLLKPHTT ; SSPPDMSPFF ; LRQYVKGHAA ; 3200: DVFEAYTQLL ; TEMVLRLPYQ ; IKKITDTNSR ; IPPPVFDHSW ; FYFLSEYLMI ; 3250: QQTPFVRRQV ; RKLLLFICGS ; KEKYRQLRDL ; HTLDSHVRGI ; KKLLEEQGIF ; 3300: LRASVVTASS ; GSALQYDTLI ; SLMEHLKACA ; EIAAQRTINW ; QKFCIKDDSV ; 3350: LYFLLQVSFL ; VDEGVSPVLL ; QLLSCALCGS ; KVLAALAASS ; GSSSASSSSA ; 3400: PVAASSGQAT ; TQSKSSTKKS ; KKEEKEKEKD ; GETSGSQEDQ ; LCTALVNQLN ; 3450: KFADKETLIQ ; FLRCFLLESN ; SSSVRWQAHC ; LTLHIYRNSS ; KSQQELLLDL ; 3500: MWSIWPELPA ; YGRKAAQFVD ; LLGYFSLKTP ; QTEKKLKEYS ; QKAVEILRTQ ; 3550: NHILTNHPNS ; NIYNTLSGLV ; EFDGYYLESD ; PCLVCNNPEV ; PFCYIKLSSI ; 3600: KVDTRYTTTQ ; QVVKLIGSHT ; ISKVTVKIGD ; LKRTKMVRTI ; NLYYNNRTVQ ; 3650: AIVELKNKPA ; RWHKAKKVQL ; TPGQTEVKID ; LPLPIVASNL ; MIEFADFYEN ; 3700: YQASTETLQC ; PRCSASVPAN ; PGVCGNCGEN ; VYQCHKCRSI ; NYDEKDPFLC ; 3750: NACGFCKYAR ; FDFMLYAKPC ; CAVDPIENEE ; DRKKAVSNIN ; TLLDKADRVY ; 3800: HQLMGHRPQL ; ENLLCKVNEA ; APEKPQDDSG ; TAGGISSTSA ; SVNRYILQLA ; 3850: QEYCGDCKNS ; FDELSKIIQK ; VFASRKELLE ; YDLQQREAAT ; KSSRTSVQPT ; 3900: FTASQYRALS ; VLGCGHTSST ; KCYGCASAVT ; EHCITLLRAL ; ATNPALRHIL ; 3950: VSQGLIRELF ; DYNLRRGAAA ; MREEVRQLMC ; LLTRDNPEAT ; QQMNDLIIGK ; 4000: VSTALKGHWA ; NPDLASSLQY ; EMLLLTDSIS ; KEDSCWELRL ; RCALSLFLMA ; 4050: VNIKTPVVVE ; NITLMCLRIL ; QKLIKPPAPT ; SKKNKDVPVE ; ALTTVKPYCN ; 4100: EIHAQAQLWL ; KRDPKASYDA ; WKKCLPIRGI ; DGNGKAPSKS ; ELRHLYLTEK ; 4150: YVWRWKQFLS ; RRGKRTSPLD ; LKLGHNNWLR ; QVLFTPATQA ; ARQAACTIVE ; 4200: ALATIPSRKQ ; QVLDLLTSYL ; DELSIAGECA ; AEYLALYQKL ; ITSAHWKVYL ; 4250: AARGVLPYVG ; NLITKEIARL ; LALEEATLST ; DLQQGYALKS ; LTGLLSSFVE ; 4300: VESIKRHFKS ; RLVGTVLNGY ; LCLRKLVVQR ; TKLIDETQDM ; LLEMLEDMTT ; 4350: GTESETKAFM ; AVCIETAKRY ; NLDDYRTPVF ; IFERLCSIIY ; PEENEVTEFF ; 4400: VTLEKDPQQE ; DFLQGRMPGN ; PYSSNEPGIG ; PLMRDIKNKI ; CQDCDLVALL ; 4450: EDDSGMELLV ; NNKIISLDLP ; VAEVYKKVWC ; TTNEGEPMRI ; VYRMRGLLGD ; 4500: ATEEFIESLD ; STTDEEEDEE ; EVYKMAGVMA ; QCGGLECMLN ; RLAGIRDFKQ ; 4550: GRHLLTVLLK ; LFSYCVKVKV ; NRQQLVKLEM ; NTLNVMLGTL ; NLALVAEQES ; 4600: KDSGGAAVAE ; QVLSIMEIIL ; DESNAEPLSE ; DKGNLLLTGD ; KDQLVMLLDQ ; 4650: INSTFVRSNP ; SVLQGLLRII ; PYLSFGEVEK ; MQILVERFKP ; YCNFDKYDED ; 4700: HSGDDKVFLD ; CFCKIAAGIK ; NNSNGHQLKD ; LILQKGITQN ; ALDYMKKHIP ; 4750: SAKNLDADIW ; KKFLSRPALP ; FILRLLRGLA ; IQHPGTQVLI ; GTDSIPNLHK ; 4800: LEQVSSDEGI ; GTLAENLLEA ; LREHPDVNKK ; IDAARRETRA ; EKKRMAMAMR ; 4850: QKALGTLGMT ; TNEKGQVVTK ; TALLKQMEEL ; IEEPGLTCCI ; CREGYKFQPT ; 4900: KVLGIYTFTK ; RVALEEMENK ; PRKQQGYSTV ; SHFNIVHYDC ; HLAAVRLARG ; 4950: REEWESAALQ ; NANTKCNGLL ; PVWGPHVPES ; AFATCLARHN ; TYLQECTGQR ; 5000: EPTYQLNIHD ; IKLLFLRFAM ; EQSFSADTGG ; GGRESNIHLI ; PYIIHTVLYV ; 5050: LNTTRATSRE ; EKNLQGFLEQ ; PKEKWVESAF ; EVDGPYYFTV ; LALHILPPEQ ; 5100: WRATRVEILR ; RLLVTSQARA ; VAPGGATRLT ; DKAVKDYSAY ; RSSLLFWALV ; 5150: DLIYNMFKKV ; PTSNTEGGWS ; CSLAEYIRHN ; DMPIYEAADK ; ALKTFQEEFM ; 5183: PVETFSEFLD ; VAGLLSEITD ; PESFLKDLLN ; SVP
Sequence Length	5183
String	Q5T4S7
Sub Unit	Interacts with RB1 and calmodulin. Interacts with protein E7 from papilloma virus HPV-16, HPV-6B and HPV-11.

Uniprot	Q5T4S7
Gene Name	UBR4
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:23352
Unigene	Hs.148078

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