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ELISA Kit for Human E3 ubiquitin-protein ligase SMURF2

SKU : BHE13811094

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E2373h
Alternative Names	HECT-type E3 ubiquitin transferase SMURF2 ; SMAD ubiquitination regulatory factor 2 ; SMAD-specific E3 ubiquitin-protein ligase 2
Categories	Elisa
Function	E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
Specificity	Natural and recombinant Human E3 ubiquitin-protein ligase SMURF2
Subcellular Location	Nucleus Cytoplasm Cell membrane Membrane raft Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
Detection Range	0.78-50ng/mL
Entryname	SMUF2_HUMAN
Method Type	Competitive
Platform	PF00168
Protein Name	E3 ubiquitin-protein ligase SMURF2
Sensitivity	0.28ng/mL
Sequence	50: MSNPGGRRNG ; PVKLRLTVLC ; AKNLVKKDFF ; RLPDPFAKVV ; VDGSGQCHST ; 100: DTVKNTLDPK ; WNQHYDLYIG ; KSDSVTISVW ; NHKKIHKKQG ; AGFLGCVRLL ; 150: SNAINRLKDT ; GYQRLDLCKL ; GPNDNDTVRG ; QIVVSLQSRD ; RIGTGGQVVD ; 200: CSRLFDNDLP ; DGWEERRTAS ; GRIQYLNHIT ; RTTQWERPTR ; PASEYSSPGR ; 250: PLSCFVDENT ; PISGTNGATC ; GQSSDPRLAE ; RRVRSQRHRN ; YMSRTHLHTP ; 300: PDLPEGYEQR ; TTQQGQVYFL ; HTQTGVSTWH ; DPRVPRDLSN ; INCEELGPLP ; 350: PGWEIRNTAT ; GRVYFVDHNN ; RTTQFTDPRL ; SANLHLVLNR ; QNQLKDQQQQ ; 400: QVVSLCPDDT ; ECLTVPRYKR ; DLVQKLKILR ; QELSQQQPQA ; GHCRIEVSRE ; 450: EIFEESYRQV ; MKMRPKDLWK ; RLMIKFRGEE ; GLDYGGVARE ; WLYLLSHEML ; 500: NPYYGLFQYS ; RDDIYTLQIN ; PDSAVNPEHL ; SYFHFVGRIM ; GMAVFHGHYI ; 550: DGGFTLPFYK ; QLLGKSITLD ; DMELVDPDLH ; NSLVWILEND ; ITGVLDHTFC ; 600: VEHNAYGEII ; QHELKPNGKS ; IPVNEENKKE ; YVRLYVNWRF ; LRGIEAQFLA ; 650: LQKGFNEVIP ; QHLLKTFDEK ; ELELIICGLG ; KIDVNDWKVN ; TRLKHCTPDS ; 700: NIVKWFWKAV ; EFFDEERRAR ; LLQFVTGSSR ; VPLQGFKALQ ; GAAGPRLFTI ; 748: HQIDACTNNL ; PKAHTCFNRI ; DIPPYESYEK ; LYEKLLTAIE ; ETCGFAVE
Sequence Length	748
String	Q9HAU4
Sub Unit	Interacts (via WW domains) with SMAD1. Interacts (via WW domains) with SMAD2 (via PY-motif). Interacts (via WW domains) with SMAD3 (via PY-motif). Interacts with SMAD6. Interacts with SMAD7 (via PY-motif) and TGFBR1; SMAD7 recruits SMURF2 to the TGF-beta receptor and regulates its degradation. Does not interact with SMAD4; SMAD4 lacks a PY-motif. Interacts with AIMP1. Interacts with STAMBP and RNF11. Interacts with NDFIP1 and NDFIP2 (Probable); this interaction activates the E3 ubiquitin-protein ligase.

Uniprot	Q9HAU4
Gene Name	SMURF2
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:64750
Unigene	Hs.515011

Reference	1. "Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF-beta receptor for degradation." Kavsak P., Rasmussen R.K., Causing C.G., Bonni S., Zhu H., Thomsen G.H., Wrana J.L. Mol. Cell6:1365-1375(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];MUTAGENESIS OF 251-PRO--VAL-284 AND 297-GLY--LEU-330;INTERACTION WITH SMAD7 AND TGFBR1 2. "Smurf2 Is a ubiquitin E3 ligase mediating proteasome-dependent degradation of Smad2 in transforming growth factor-beta signaling." Lin X., Liang M., Feng X.-H. J. Biol. Chem.275:36818-36822(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];MUTAGENESIS OF 251-PRO--VAL-284 AND CYS-716 3. "Regulation of Smad degradation and activity by Smurf2, an E3 ubiquitin ligase." Zhang Y., Chang C., Gehling D.J., Hemmati-Brivanlou A., Derynck R. Proc. Natl. Acad. Sci. U.S.A.98:974-979(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];MUTAGENESIS OF CYS-716 4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] tissue: Brain. 5. "TGF-beta induces assembly of a Smad2-Smurf2 ubiquitin ligase complex that targets SnoN for degradation." Bonni S., Wang H.R., Causing C.G., Kavsak P., Stroschein S.L., Luo K., Wrana J.L. Nat. Cell Biol.3:587-595(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH SMAD2; SMAD3 AND SNON 6. "Distinct endocytic pathways regulate TGF-beta receptor signalling and turnover." Di Guglielmo G.M., Le Roy C., Goodfellow A.F., Wrana J.L. Nat. Cell Biol.5:410-421(2003) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;MUTAGENESIS OF CYS-716 7. Di Guglielmo G.M., Le Roy C., Goodfellow A.F., Wrana J.L. Nat. Cell Biol.5:680-680(2003) [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM 8. "The RING-H2 protein RNF11 is overexpressed in breast cancer and is a target of Smurf2 E3 ligase." Subramaniam V., Li H., Wong M.J., Kitching R., Attisano L., Wrana J., Zubovits J., Burger A.M., Seth A.K. Br. J. Cancer89:1538-1544(2003) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH RNF11 9. "An RNF11: Smurf2 complex mediates ubiquitination of the AMSH protein." Li H., Seth A.K. Oncogene23:1801-1808(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH STAMBP AND RNF11 10. "AIMP1/p43 downregulates TGF-beta signaling via stabilization of smurf2." Lee Y.S., Han J.M., Son S.H., Choi J.W., Jeon E.J., Bae S.-C., Park Y.I., Kim S. Biochem. Biophys. Res. Commun.371:395-400(2008) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH AIMP1 11. "Control of the activity of WW-HECT domain E3 ubiquitin ligases by NDFIP proteins." Mund T., Pelham H.R. EMBO Rep.10:501-507(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACTIVATION BY NDFIP1 AND NDFIP2;AUTOUBIQUITINATION 12. "SCF(FBXL15) regulates BMP signalling by directing the degradation of HECT-type ubiquitin ligase Smurf1." Cui Y., He S., Xing C., Lu K., Wang J., Xing G., Meng A., Jia S., He F., Zhang L. EMBO J.30:2675-2689(2011) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION 13. "Regulation of Smurf2 ubiquitin ligase activity by anchoring the E2 to the HECT domain." Ogunjimi A.A., Briant D.J., Pece-Barbara N., Le Roy C., Di Guglielmo G.M., Kavsak P., Rasmussen R.K., Seet B.T., Sicheri F., Wrana J.L. Mol. Cell19:297-308(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 369-748;INTERACTION WITH SMAD7;MUTAGENESIS OF TRP-535; HIS-547 AND TYR-581 14. "An expanded WW domain recognition motif revealed by the interaction between Smad7 and the E3 ubiquitin ligase Smurf2." Chong P.A., Lin H., Wrana J.L., Forman-Kay J.D. J. Biol. Chem.281:17069-17075(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 297-333 IN COMPLEX WITH SMAD7 15. "Autoinhibition of the HECT-type ubiquitin ligase Smurf2 through its C2 domain." Wiesner S., Ogunjimi A.A., Wang H.R., Rotin D., Sicheri F., Wrana J.L., Forman-Kay J.D. Cell130:651-662(2007) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 10-140;AUTOINHIBITION BY C2 DOMAIN;MUTAGENESIS OF PHE-29; PHE-30; THR-56 AND LEU-57

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