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ELISA Kit for Human E3 ubiquitin-protein ligase AMFR

SKU : BHE13801168

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E14974h
Alternative Names	Autocrine motility factor receptor ; AMF receptor ; RING finger protein 45 ; RING-type E3 ubiquitin transferase AMFR ; gp78 ; RNF45
Categories	Elisa
Function	E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.
Specificity	Natural and recombinant Human E3 ubiquitin-protein ligase AMFR
Subcellular Location	Endoplasmic reticulum membrane Multi-pass membrane protein
Detection Range	0.625-40ng/mL
Entryname	AMFR_HUMAN
Method Type	Sandwich
Platform	PF02845
Protein Name	E3 ubiquitin-protein ligase AMFR
Sensitivity	0.34ng/ml
Sequence	50: MPLLFLERFP ; WPSLRTYTGL ; SGLALLGTII ; SAYRALSQPE ; AGPGEPDQLT ; 100: ASLQPEPPAP ; ARPSAGGPRA ; RDVAQYLLSD ; SLFVWVLVNT ; ACCVLMLVAK ; 150: LIQCIVFGPL ; RVSERQHLKD ; KFWNFIFYKF ; IFIFGVLNVQ ; TVEEVVMWCL ; 200: WFAGLVFLHL ; MVQLCKDRFE ; YLSFSPTTPM ; SSHGRVLSLL ; VAMLLSCCGL ; 250: AAVCSITGYT ; HGMHTLAFMA ; AESLLVTVRT ; AHVILRYVIH ; LWDLNHEGTW ; 300: EGKGTYVYYT ; DFVMELTLLS ; LDLMHHIHML ; LFGNIWLSMA ; SLVIFMQLRY ; 350: LFHEVQRRIR ; RHKNYLRVVG ; NMEARFAVAT ; PEELAVNNDD ; CAICWDSMQA ; 400: ARKLPCGHLF ; HNSCLRSWLE ; QDTSCPTCRM ; SLNIADNNRV ; REEHQGENLD ; 450: ENLVPVAAAE ; GRPRLNQHNH ; FFHFDGSRIA ; SWLPSFSVEV ; MHTTNILGIT ; 500: QASNSQLNAM ; AHQIQEMFPQ ; VPYHLVLQDL ; QLTRSVEITT ; DNILEGRIQV ; 550: PFPTQRSDSI ; RPALNSPVER ; PSSDQEEGET ; SAQTERVPLD ; LSPRLEETLD ; 600: FGEVEVEPSE ; VEDFEARGSR ; FSKSADERQR ; MLVQRKDELL ; QQARKRFLNK ; 643: SSEDDAASES ; FLPSEGASSD ; PVTLRRRMLA ; AAAERRLQKQ ; QTS
Sequence Length	643
Sub Unit	Interacts with RNF5. Also forms an ERAD complex containing VCP/p97, NGLY1; PSMC1; SAKS1 AND RAD23B required for coupling retrotranslocation, ubiquitination and deglycosylation (By similarity). Interacts with DRL1. Interacts (through a region distinct from the RING finger) with UBE2G2/UBC7. Component of the VCP/p97-AMFR/gp78 complex that enhances VCP/p97 binding to polyubiquitinated proteins for their degradation by the endoplasmic reticulum-associated degradation (ERAD) pathway. Interacts (via the VIM) with VCP/p97. Interacts (via its membrane domain) with INSIG1; the interaction initiates the sterol-mediated ubiquitination and degradation of HMGCR by the ERAD pathway.

Uniprot	Q9UKV5
Gene Name	AMFR
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:267
Unigene	Hs.295137

Reference	1. "The autocrine motility factor receptor gene encodes a novel type of seven transmembrane protein." Shimizu K., Tani M., Watanabe H., Nagamachi Y., Niinaka Y., Shiroishi T., Ohwada S., Raz A., Yokota J. FEBS Lett.456:295-300(1999) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION 2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] tissue: Ovary. 3. "Identification of an upstream region that controls the transcription of the human autocrine motility factor receptor." Huang B., Xie Y., Raz A. Biochem. Biophys. Res. Commun.212:727-742(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 358-643 tissue: Placenta. 4. "Purification of human tumor cell autocrine motility factor and molecular cloning of its receptor." Watanabe H., Carmi P., Hogan V., Raz T., Silletti S., Nabi I.R., Raz A. J. Biol. Chem.266:13442-13448(1991) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 520-643 5. "The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum." Fang S., Ferrone M., Yang C., Jensen J.P., Tiwari S., Weissman A.M. Proc. Natl. Acad. Sci. U.S.A.98:14422-14427(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION AS A UBIQUITIN LIGASE;SUBCELLULAR LOCATION;INTERACTION WITH UBE2G2 6. "Overexpression of the tumor autocrine motility factor receptor, gp78, a ubiquitin protein ligase (E3), results in increased ubiquitinylation and decreased secretion of apolipoprotein B100 in Hep G2 cells." Liang J.S., Kim T., Fang S., Yamaguchi J., Weissman A.M., Fisher E.A., Ginsberg H.N. J. Biol. Chem.278:23984-23988(2003) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION OF APOB 7. "Gp78, a membrane-anchored ubiquitin ligase, associates with Insig-1 and couples sterol-regulated ubiquitination to degradation of HMG CoA reductase." Song B.L., Sever N., DeBose-Boyd R.A. Mol. Cell19:829-840(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH INSIG1 AND VCP;FUNCTION;MUTAGENESIS OF CYS-356 8. "Recruitment of the p97 ATPase and ubiquitin ligases to the site of retrotranslocation at the endoplasmic reticulum membrane." Ye Y., Shibata Y., Kikkert M., van Voorden S., Wiertz E., Rapoport T.A. Proc. Natl. Acad. Sci. U.S.A.102:14132-14138(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH DERL1 AND VCP 9. "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-516;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS] tissue: Cervix carcinoma. 10. "CYP3A4 ubiquitination by gp78 (the tumor autocrine motility factor receptor, AMFR) and CHIP E3 ligases." Pabarcus M.K., Hoe N., Sadeghi S., Patterson C., Wiertz E., Correia M.A. Arch. Biochem. Biophys.483:66-74(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION 11. "Solution structure of RSGI RUH-076, a human CUE domain." RIKEN structural genomics initiative (RSGI) Submitted (2007-09) to the PDB data bank [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 452-502 12. "The structural and functional basis of the p97/valosin-containing protein (VCP)-interacting motif (VIM): mutually exclusive binding of cofactors to the N-terminal domain of p97." Hanzelmann P., Schindelin H. J. Biol. Chem.286:38679-38690(2011) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 622-640 IN COMPLEX WITH VCP;VIM MOTIF 13. "The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V., Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.more... Science314:268-274(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT [LARGE SCALE ANALYSIS] VAL-605

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