ELISA Kit for Human Catalase

Mfr.No.

E0242h

Categories

Elisa

Function

Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.

Specificity

Natural and recombinant Human Catalase

Subcellular Location

Peroxisome

Detection Range

0.312-20ng/mL

Entryname

CATA_HUMAN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CATA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-104%	90-107%	94-102%	91-105%
EDTA plasma(n=5)	94-103%	91-109%	95-104%	90-110%
heparin plasma(n=5)	93-107%	93-105%	92-108%	95-103%

Method Type

Sandwich

Platform

PF00199

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.7%

Protein Name

Catalase

Recovery

Recovery was determined by spiking various levels of CATA into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	98	90-104
Plasma	99	91-105

Sensitivity

0.085ng/mL

Sequence

50:

MADSRDPASD ; QMQHWKEQRA ; AQKADVLTTG ; AGNPVGDKLN ; VITVGPRGPL ;

100:

LVQDVVFTDE ; MAHFDRERIP ; ERVVHAKGAG ; AFGYFEVTHD ; ITKYSKAKVF ;

150:

EHIGKKTPIA ; VRFSTVAGES ; GSADTVRDPR ; GFAVKFYTED ; GNWDLVGNNT ;

200:

PIFFIRDPIL ; FPSFIHSQKR ; NPQTHLKDPD ; MVWDFWSLRP ; ESLHQVSFLF ;

250:

SDRGIPDGHR ; HMNGYGSHTF ; KLVNANGEAV ; YCKFHYKTDQ ; GIKNLSVEDA ;

300:

ARLSQEDPDY ; GIRDLFNAIA ; TGKYPSWTFY ; IQVMTFNQAE ; TFPFNPFDLT ;

350:

KVWPHKDYPL ; IPVGKLVLNR ; NPVNYFAEVE ; QIAFDPSNMP ; PGIEASPDKM ;

400:

LQGRLFAYPD ; THRHRLGPNY ; LHIPVNCPYR ; ARVANYQRDG ; PMCMQDNQGG ;

450:

APNYYPNSFG ; APEQQPSALE ; HSIQYSGEVR ; RFNTANDDNV ; TQVRAFYVNV ;

500:

LNEEQRKRLC ; ENIAGHLKDA ; QIFIQKKAVK ; NFTEVHPDYG ; SHIQALLDKY ;

527:

NAEKPKNAIH ; TFVQSGSHLA ; AREKANL

Sequence Length

527

String

P04040

Sub Unit

Homotetramer.

Uniprot	P04040
Gene Name	CAT
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:847
Unigene	Hs.502302

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SKU	Supplier No.	Size	Your price	Quantity
BHE13801884	E0242h	96T	$736		Add to Cart

ELISA Kit for Human Catalase

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