ELISA Kit for Human 3-hydroxyisobutyrate dehydrogenase, mitochondrial

Mfr.No.

E12565h

Categories

Elisa

Specificity

Natural and recombinant Human 3-hydroxyisobutyrate dehydrogenase, mitochondrial

Subcellular Location

Mitochondrion

Detection Range

0.156-10ng/mL

Entryname

3HIDH_HUMAN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIBADH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-108%	94-111%	95-107%	98-105%
EDTA plasma(n=5)	92-107%	96-106%	90-109%	92-107%
heparin plasma(n=5)	95-103%	93-107%	94-107%	93-108%

Method Type

Sandwich

Platform

PF03446

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Protein Name

3-hydroxyisobutyrate dehydrogenase, mitochondrial

Recovery

Recovery was determined by spiking various levels of HIBADH into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	102	93-105
Plasma	100	91-106

Sensitivity

0.08ng/mL

Sequence

50:

MAASLRLLGA ; ASGLRYWSRR ; LRPAAGSFAA ; VCSRSVASKT ; PVGFIGLGNM ;

100:

GNPMAKNLMK ; HGYPLIIYDV ; FPDACKEFQD ; AGEQVVSSPA ; DVAEKADRII ;

150:

TMLPTSINAI ; EAYSGANGIL ; KKVKKGSLLI ; DSSTIDPAVS ; KELAKEVEKM ;

200:

GAVFMDAPVS ; GGVGAARSGN ; LTFMVGGVED ; EFAAAQELLG ; CMGSNVVYCG ;

250:

AVGTGQAAKI ; CNNMLLAISM ; IGTAEAMNLG ; IRLGLDPKLL ; AKILNMSSGR ;

300:

CWSSDTYNPV ; PGVMDGVPSA ; NNYQGGFGTT ; LMAKDLGLAQ ; DSATSTKSPI ;

336:

LLGSLAHQIY ; RMMCAKGYSK ; KDFSSVFQFL ; REEETF

Sequence Length

336

String

P31937

Sub Unit

Homodimer.

Uniprot	P31937
Gene Name	HIBADH
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:11112
Unigene	Hs.406758

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SKU	Supplier No.	Size	Your price	Quantity
BHE13800054	E12565h	96T	$736		Add to Cart

ELISA Kit for Human 3-hydroxyisobutyrate dehydrogenase, mitochondrial

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