ELISA Kit for Dog Vascular endothelial growth factor A

Mfr.No.

E0143d

Alternative Names

Vascular permeability factor ; VPF ; VEGF

Categories

Elisa

Function

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin.

Specificity

Natural and recombinant Dog Vascular endothelial growth factor A

Subcellular Location

Secreted Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Detection Range

31.2-2000pg/mL

Entryname

VEGFA_CANFA

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF-A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	84-93%	79-94%	88-96%	78-91%
EDTA plasma(n=5)	78-89%	82-97%	90-102%	89-103%
heparin plasma(n=5)	83-94%	89-101%	87-99%	91-103%

Method Type

Sandwich

Platform

PF00341

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 10.2%

Protein Name

Vascular endothelial growth factor A

Recovery

Recovery was determined by spiking various levels of VEGF-A into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	86	80-95
Plasma	95	90-102

Sensitivity

10.9pg/mL

Sequence

50:

MNFLLSWVHW ; SLALLLYLHH ; AKWSQAAPMA ; GGEHKPHEVV ; KFMDVYQRSY ;

100:

CRPIETLVDI ; FQEYPDEIEY ; IFKPSCVPLM ; RCGGCCNDEG ; LECVPTEEFN ;

150:

ITMQIMRIKP ; HQGQHIGEMS ; FLQHSKCECR ; PKKDRARQEK ; KSIRGKGKGQ ;

200:

KRKRKKSRYK ; PWSVPCGPCS ; ERRKHLFVQD ; PQTCKCSCKN ; TDSRCKARQL ;

214:

ELNERTCRCD ; KPRR

Sequence Length

214

String

Q9MYV3

Sub Unit

Homodimer; disulfide-linked. Also found as heterodimer with PGF.

Uniprot	Q9MYV3
Gene Name	VEGFA
Immunogen Species	Canis Lupus Familiaris (Dog)
Kegg	cfa:403802
Unigene	Cfa.3581

Reference	1. "Vascular endothelial growth factor (VEGF) and its receptors in tumor-bearing dogs." Scheidegger P., Weiglhofer W., Suarez S., Kaser-Hotz B., Steiner R., Ballmer-Hofer K., Jaussi R. Biol. Chem.380:1449-1454(1999) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-188) 2. Jingjing L., Roque R.S. Submitted (1999-03) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-188; VEGF-182 AND VEGF-164) tissue: Heart.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13811397	E0143d	96T	$900		Add to Cart

ELISA Kit for Dog Vascular endothelial growth factor A

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