ELISA Kit for Dog Occludin

Mfr.No.

E14928d

Categories

Elisa

Function

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. Interacts with ZO-1.

Specificity

Natural and recombinant Dog Occludin

Subcellular Location

Membrane Multi-pass membrane protein Cell junction tight junction

Entryname

OCLN_CANFA

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OCLN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-102%	90-103%	93-98%	91-100%
EDTA plasma(n=5)	90-106%	93-101%	91-102%	93-102%
heparin plasma(n=5)	93-103%	94-100%	92-100%	91-104%

Method Type

Sandwich

Platform

PF01284

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.7%

Protein Name

Occludin

Recovery

Recovery was determined by spiking various levels of OCLN into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	91-103
Plasma	97	93-106

Sequence

50:

MSSRPFESPP ; PYRPDEFKPN ; HYAPSNDVYG ; GDMHVRPMLS ; QPAYSFYPED ;

100:

EILHFYKWTS ; PPGVIRILSM ; LVIVMCIAIF ; GCVASTLAWD ; RGYGTGLMGG ;

150:

SIGYPYGSGF ; GSYGTGYGYG ; FGYGYGYGGY ; TDPRAAKGFL ; LAMVAFCFIA ;

200:

ALVIFVTSVI ; RSDISRTRRY ; YLTVIILSAF ; LGVMMFIATI ; VYIMGVNPTA ;

250:

QASGSLYSSQ ; IYAMCNQFYA ; STATGLYMDQ ; YLYHYCVVDP ; QEAIAIVLGF ;

300:

MVIVAFALII ; FFAVKTRRKM ; DRYDKSNILW ; DKEHIYDEQP ; PNVEEWVKNV ;

350:

SAGTQDMPPP ; PSDYVERVDS ; PMAYSSNGKV ; NDKRLYPESS ; YKSTPVPEVV ;

400:

QELPATSPAD ; DFRQPRYSSS ; GHLEPPSKRA ; PSKGRTGRPK ; RLEQDHYETD ;

450:

YTTGGESCDE ; LEEDWIREYP ; PITSDQQRQL ; YKRNFDTGLQ ; EYKSLQAELD ;

500:

EINKELSRLD ; KELDDYREES ; EEYMAAADEY ; NRLKQVKGSP ; DYKNKRNYCK ;

521:

QLKSKLSHIK ; KMVGDYDRQK ; T

Sequence Length

521

String

Q28269

Sub Unit

Interacts with VAPA.

Uniprot	Q28269
Gene Name	OCLN
Immunogen Species	Canis Lupus Familiaris (Dog)
Kegg	cfa:403844
Unigene	Cfa.3606

Reference	1. "Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues." Ando-Akatsuka Y., Saitou M., Hirase T., Kishi M., Sakakibara A., Itoh M., Yonemura S., Furuse M., Tsukita S. J. Cell Biol.133:43-47(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Cocker spaniel. tissue: Kidney. 2. "Possible involvement of phosphorylation of occludin in tight junction formation." Sakakibara A., Furuse M., Saitou M., Ando-Akatsuka Y., Tsukita S. J. Cell Biol.137:1393-1401(1997) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION

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SKU	Supplier No.	Size	Your price	Quantity
BHE13808348	E14928d	96T	$900		Add to Cart

ELISA Kit for Dog Occludin

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