ELISA Kit for Chicken Talin-1

Mfr.No.

E2104c

Alternative Names

TLN

Categories

Elisa

Function

Probably involved in connections of major cytoskeletal structures to the plasma membrane. Talin is a high molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.

Specificity

Natural and recombinant Chicken Talin-1

Subcellular Location

Cell projection ruffle membrane Peripheral membrane protein Cytoplasmic side Cytoplasm cytoskeleton Colocalizes with LAYN at the membrane ruffles.

Detection Range

0.78-50ng/mL

Entryname

TLN1_CHICK

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TLN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-102%	93-100%	89-102%	91-100%
Saliva(n=3)	94-99%	91-103%	91-105%	90-102%
heparin plasma(n=5)	91-102%	92-104%	93-101%	93-100%

Method Type

Competitive

Platform

PF00373

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.3%

Protein Name

Talin-1

Recovery

Recovery was determined by spiking various levels of TLN1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	92-104
Plasma	97	93-102

Sensitivity

0.23ng/mL

Sequence

50:

MVALSLKISI ; GNVVKTMQFE ; PSTMVYDACR ; MIRERVPEAQ ; MGQPNDFGLF ;

100:

LSDEDPKKGI ; WLEAGKALDY ; YMLRNGDTME ; YKKKQRPLKI ; RMLDGTVKTV ;

150:

MVDDSKTVTD ; MLTTICARIG ; ITNYDEYSLV ; REIMEEKKEE ; VTGTLKKDKT ;

200:

LLRDEKKMEK ; LKQKLHTDDE ; LNWLDHGRTL ; REQGIDDNET ; LLLRRKFFYS ;

250:

DQNVDSRDPV ; QLNLLYVQAR ; DDILNGSHPV ; SFDKACEFAG ; YQCQIQFGPH ;

300:

NEQKHKPGFL ; ELKDFLPKEY ; IKQKGERKIF ; MAHKNCGNMS ; EIEAKVRYVK ;

350:

LARSLKTYGV ; SFFLVKEKMK ; GKNKLVPRLL ; GITKECVMRV ; DEKTKEVIQE ;

400:

WSLTNIKRWA ; ASPKSFTLDF ; GDYQDGYYSV ; QTTEGEQIAQ ; LIAGYIDIIL ;

450:

KKKKSKDHFG ; LEGDEESTML ; EDSVSPKKST ; VLQQQFNRVG ; KAELGSVALP ;

500:

AIMRTGAGGP ; ENFQVGTMPQ ; AQMQITSGQM ; HRGHMPPLTS ; AQQALTGTIN ;

550:

SSMQAVNAAQ ; ATLDDFETLP ; PLGQDAASKA ; WRKNKMDESK ; HEIHSQADAI ;

600:

TAGTASVVNL ; TAGDPADTDY ; TAVGCAVTTI ; SSNLTEMSKG ; VKLLAALMED ;

650:

EGGNGRQLLQ ; AAKNLASAVS ; DLLKTAQPAS ; AEPRQNLLQA ; AGLVGQTSGE ;

700:

LLQQIGESDT ; DPRFQDMLMQ ; LAKAVASAAA ; ALVLKAKNVA ; QKTEDSALQT ;

750:

QVIAAATQCA ; LSTSQLVACT ; KVVAPTISSP ; VCQEQLIEAG ; KLVAKSAEGC ;

800:

VEASKAATND ; DQLLKQVGVA ; ATAVTQALND ; LLQHIKQHAT ; GGQPIGRYDQ ;

850:

ATDTILNVTE ; NIFSSMGDAG ; EMVRQARILA ; QATSDLVNAI ; KADAEGETDL ;

900:

ENSRKLLSAA ; KILADATAKM ; VEAAKGAAAH ; PDSEEQQQRL ; REAAEGLRMA ;

950:

TNAAAQNAIK ; KKLVHKLEHA ; AKQAAASATQ ; TIAAAQHAAA ; SNKNPAAQQQ ;

1000:

LVQSCKVVAD ; QIPMLVQGVR ; GSQSQPDSPS ; AQLALIAASQ ; NFLQPGGKMV ;

1050:

AAAKATVPTI ; TDQASAMQLS ; QCAKNLAAAL ; AELRTAAQKA ; QEACGPLEID ;

1100:

SALGLVQSLE ; RDLKEAKAAA ; RDGKLKPLPG ; ETMEKCAQDL ; GNSTKAVTSA ;

1150:

IAHLLGEVAQ ; GNENYTGIAA ; REVAQALRSL ; SQAARGVAAN ; SSDPQAQNAM ;

1200:

LECASDVMDK ; ANNLIEEARK ; AVAKPGDPDS ; QQRLVQVAKA ; VSQALNRCVN ;

1250:

CLPGQRDVDA ; AIRMVGEASK ; RLLSDSFPPS ; NKTFQEAQSQ ; LNRAAAGLNQ ;

1300:

SANELVQASR ; GTPQDLAKSS ; GKFGQDFNEF ; LQAGVEMASL ; SPTKEDQAQV ;

1350:

VSNLKSISMS ; SSKLLLAAKA ; LSADPTSPNL ; KSQLAAAARA ; VTDSINQLIT ;

1400:

MCTQQAPGQK ; ECDNALRELE ; TVKELLENPT ; QTVNDMSYFS ; CLDSVMENSK ;

1450:

VLGESMAGIS ; QNAKNSKLPE ; FGESISAASK ; ALCGLTEAAA ; QAAYLVGVSD ;

1500:

PNSQAGQQGL ; VDPTQFARAN ; QAIQMACQNL ; VDPACTQSQV ; LSAATIVAKH ;

1550:

TSALCNTCRL ; ASSRTANPVA ; KRQFVQPAKE ; VANSTANLVK ; TIKALDGAFN ;

1600:

EENRERCRAA ; TAPLIEAVDN ; LTAFASNPEF ; ATVPAQISPE ; GRRAMEPIVT ;

1650:

SAKTMLESSA ; GLIQTARSLA ; VNPKDPPQWS ; VLAGHSRTVS ; DSIKKLITNM ;

1700:

RDKAPGQREC ; DEAIDVLNRC ; MREVDQASLA ; AISQQLAPRE ; GISQEALHNQ ;

1750:

MITAVQEINN ; LIEPVASAAR ; AEASQLGHKV ; SQMAQYFEPL ; ILAAIGAASK ;

1800:

TPNHQQQMNL ; LDQTKTLAES ; ALQMLYTAKE ; AGGNPKQAAH ; TQEALEEAVQ ;

1850:

MMKEAVEDLT ; TTLNEAASAA ; GVVGGMVDSI ; TQAINQLDEG ; PMGEPEGTFV ;

1900:

DYQTTMVKTA ; KAIAVTVQEM ; VTKSTTNPDE ; LGILANQLTN ; DYGQLAQQAK ;

1950:

PAALTAENEE ; IGSHIKRRVQ ; ELGHGCAALV ; TKAGALQCSP ; SDAYTKKELI ;

2000:

ESARKVSEKV ; SHVLAALQAG ; NRGTQACITA ; ASAVSGIIAD ; LDTTIMFATA ;

2050:

GTLNRENSET ; FADHREGILK ; TAKALVEDTK ; VLVQNATASQ ; EKLAQAAQSS ;

2100:

VSTITRLAEV ; VKLGAASLGS ; EDPETQVVLI ; NAVKDVAKAL ; GDLIGATKAA ;

2150:

AGKAGDDPAV ; YQLKNSAKVM ; VTNVTSLLKT ; VKAVEDEATK ; GTRALEATIE ;

2200:

HIRQELAVFS ; SPVPPAQVST ; PEDFIRMTKG ; ITMATAKAVA ; AGNSCRQEDV ;

2250:

IATANLSRRA ; IADMLRACKE ; AAYHPEVSAD ; VRQRALRFGK ; ECADGYLELL ;

2300:

EHVLVILQKP ; THELKQQLAG ; YSKRVASSVT ; ELIQAAEAMK ; GTEWVDPEDP ;

2350:

TVIAENELLG ; AAAAIEAAAK ; KLEQLKPRAK ; PKQADESLDF ; EEQILEAAKS ;

2400:

IAAATSALVK ; AASAAQRELV ; AQGKVGVIPA ; NAVDDGQWSQ ; GLISAARMVA ;

2450:

AATNNLCEAA ; NAAVQGHASE ; EKLISSAKQV ; AASTAQLLVA ; CKVKADHDSE ;

2500:

AMKRLQAAGN ; AVKRASDNLV ; KAAQKAAAFQ ; DHDETVVVKE ; KMVGGIAQII ;

2541:

AAQEEMLRKE ; RELEEARKKL ; AMIRQQQYKF ; LPTELRDEEQ ; N

Sequence Length

2541

String

P54939

Sub Unit

Interacts with PIP5K1C and NRAP (By similarity). Binds with high affinity to vinculin and with low affinity to integrins. May interact with F-actin. Interacts with LAYN.

Uniprot	P54939
Gene Name	TLN1
Immunogen Species	Gallus Gallus (Chicken)
Kegg	gga:395194
Unigene	Gga.4319

Reference	1. "Talin contains three actin-binding sites each of which is adjacent to a vinculin-binding site." Hemmings L., Rees D.J.G., Ohanian V., Bolton S.J., Gilmore A.P., Patel B., Priddle H., Trevithick J.E., Hynes R.O., Critchley D.R. J. Cell Sci.109:2715-2726(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];INTERACTION WITH F-ACTIN AND VCL 2. "The cytoskeletal protein talin is O-glycosylated." Hagmann J., Grob M., Burger M.M. J. Biol. Chem.267:14424-14428(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 1469-1487 AND 1882-1898;GLYCOSYLATION AT THR-1486 AND THR-1889 tissue: Gizzard. 3. "Structural determinants of integrin recognition by talin." Garcia-Alvarez B., de Pereda J.M., Calderwood D.A., Ulmer T.S., Critchley D., Campbell I.D., Ginsberg M.H., Liddington R.C. Mol. Cell11:49-58(2003) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 196-400 IN COMPLEX WITH ITGB3 4. "Vinculin activation by talin through helical bundle conversion." Izard T., Evans G., Borgon R.A., Rush C.L., Bricogne G., Bois P.R.J. Nature427:171-175(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 1944-1969 IN COMPLEX WITH VCL 5. "Layilin, a novel talin-binding transmembrane protein homologous with C-type lectins, is localized in membrane ruffles." Borowsky M.L., Hynes R.O. J. Cell Biol.143:429-442(1998) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH LAYN

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ELISA Kit for Chicken Talin-1

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