ELISA Kit for Chicken Occludin

Mfr.No.

E14928c

Categories

Elisa

Function

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. Interacts with ZO-1.

Specificity

Natural and recombinant Chicken Occludin

Subcellular Location

Membrane Multi-pass membrane protein Cell junction tight junction

Entryname

OCLN_CHICK

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OCLN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-102%	90-103%	93-98%	91-100%
EDTA plasma(n=5)	90-106%	93-101%	91-102%	93-102%
heparin plasma(n=5)	93-103%	94-100%	92-100%	91-104%

Method Type

Sandwich

Platform

PF01284

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.7%

Protein Name

Occludin

Recovery

Recovery was determined by spiking various levels of OCLN into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	91-103
Plasma	97	93-106

Sequence

50:

MFSKKSYDGP ; PAGYGPPTGY ; GAPTADYGYG ; SPPPGSYYVD ; DAPQLFYKWT ;

100:

SPPGAVRGLQ ; AGVLVLCIAI ; FACVASTLAW ; DYGYGLGGAY ; GTGLGGFYGS ;

150:

NYYGSGLSYS ; YGYGGYYGGV ; NQRTANGFMI ; AMAVLCFLAQ ; LGLLVAALSK ;

200:

SGATRSRRFY ; LAVLVLSAVL ; AFVMLIASIV ; YIMGVNPQAQ ; MSSGYYYSPL ;

250:

LAMCSQAYGS ; TYLNQYIYHY ; CTVDPQEAVA ; AVCGFLIVIL ; LCLICFFAQK ;

300:

TRSKIWRYGK ; ANIYWDRAPV ; VQEGPDVEEW ; VKNVADGASV ; QDETATLAYS ;

350:

EKPTSPVAAP ; PYSYVPPPSA ; GYYPSGTYSS ; RGDQPDRALS ; ASPVHGEEEE ;

400:

EKGKDQPSRP ; PARRGRRRRR ; NPELDESQYE ; TDYTTAVESS ; DERDQEQWAS ;

450:

LYPPITSDGA ; RQRYKQEFDT ; DLKRYKQLCA ; EMDSINDRLN ; QLSRRLDSIT ;

500:

EDSPQYQDVA ; EEYNQLKDLK ; RSPDYQSKKQ ; ESKVLRNKLF ; HIKRMVSAYD ;

504:

KVRG

Sequence Length

504

Sub Unit

Interacts with TJP1 and TJP3.

Uniprot	Q91049
Gene Name	OCLN
Immunogen Species	Gallus Gallus (Chicken)
Kegg	gga:396026
Unigene	Gga.603

Reference	1. "Occludin: a novel integral membrane protein localizing at tight junctions." Furuse M., Hirase T., Itoh M., Nagafuchi A., Yonemura S., Tsukita S., Tsukita S. J. Cell Biol.123:1777-1788(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Embryonic brain. 2. "Phosphorylation of Tyr-398 and Tyr-402 in occludin prevents its interaction with ZO-1 and destabilizes its assembly at the tight junctions." Elias B.C., Suzuki T., Seth A., Giorgianni F., Kale G., Shen L., Turner J.R., Naren A., Desiderio D.M., Rao R. J. Biol. Chem.284:1559-1569(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-379 AND TYR-383 BY SRC;IDENTIFICATION BY MASS SPECTROMETRY;MUTAGENESIS OF TYR-379 AND TYR-383;INTERACTION WITH TJP1 AND TJP3

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SKU	Supplier No.	Size	Your price	Quantity
BHE13808159	E14928c	96T	$900		Add to Cart

ELISA Kit for Chicken Occludin

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