ELISA Kit for Chicken Major prion protein homolog

Mfr.No.

E9345c

Alternative Names

65-21 protein ; Acetylcholine receptor-inducing activity ; ARIA ; PR-LP ; PRN-P

Categories

Elisa

Function

Stimulates the synthesis of acetylcholine receptors in cultured chicken myotubes. May serve normally to regulate the chemoreceptor number at the neuromuscular junction and perhaps in the central nervous system as well.

Specificity

Natural and recombinant Chicken Major prion protein homolog

Subcellular Location

Cell membrane Lipid-anchor GPI-anchor

Detection Range

0.312-20ng/mL

Entryname

PRIO_CHICK

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-102%	92-103%	95-105%	92-107%
EDTA plasma(n=5)	88-96%	93-101%	94-102%	95-106%
heparin plasma(n=5)	93-104%	94-105%	101-106%	95-109%

Method Type

Sandwich

Platform

PF00377

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.6%

Protein Name

Major prion protein homolog

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-106
Plasma	98	92-108

Sensitivity

0.095ng/mL

Sequence

50:

MARLLTTCCL ; LALLLAACTD ; VALSKKGKGK ; PSGGGWGAGS ; HRQPSYPRQP ;

100:

GYPHNPGYPH ; NPGYPHNPGY ; PHNPGYPHNP ; GYPQNPGYPH ; NPGYPGWGQG ;

150:

YNPSSGGSYH ; NQKPWKPPKT ; NFKHVAGAAA ; AGAVVGGLGG ; YAMGRVMSGM ;

200:

NYHFDSPDEY ; RWWSENSARY ; PNRVYYRDYS ; SPVPQDVFVA ; DCFNITVTEY ;

250:

SIGPAAKKNT ; SEAVAAANQT ; EVEMENKVVT ; KVIREMCVQQ ; YREYRLASGI ;

273:

QLHPADTWLA ; VLLLLLTTLF ; AMH

Sequence Length

273

String

P27177

Uniprot	P27177
Gene Name	PRNP
Immunogen Species	Gallus Gallus (Chicken)
Kegg	gga:396452
Unigene	Gga.3867

Reference	1. "Molecular cloning of a candidate chicken prion protein." Gabriel J.M., Oesch B., Kretzschmar H., Scott M., Prusiner S.B. Proc. Natl. Acad. Sci. U.S.A.89:9097-9101(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] 2. "A prion-like protein from chicken brain copurifies with an acetylcholine receptor-inducing activity." Harris D.A., Falls D.L., Johnson F.A., Fischbach G.D. Proc. Natl. Acad. Sci. U.S.A.88:7664-7668(1991) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 25-51 tissue: Brain. 3. "Mr 42,000 ARIA: a protein that may regulate the accumulation of acetylcholine receptors at developing chick neuromuscular junctions." Falls D.L., Harris D.A., Johnson F.A., Morgan M.M., Corfas G., Fischbach G.D. Cold Spring Harb. Symp. Quant. Biol.55:397-406(1990) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW 4. "Prion protein NMR structures of chickens, turtles, and frogs." Calzolai L., Lysek D.A., Perez D.R., Guentert P., Wuethrich K. Proc. Natl. Acad. Sci. U.S.A.102:651-655(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 134-248

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BHE13808866	E9345c	96T	$900		Add to Cart

ELISA Kit for Chicken Major prion protein homolog

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