ELISA Kit for Chicken Interleukin-18

Mfr.No.

E0064c

Categories

Elisa

Function

Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

Specificity

Natural and recombinant Chicken Interleukin-18

Subcellular Location

Secreted

Detection Range

7.8-500pg/mL

Entryname

IL18_CHICK

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-105%	94-101%	95-102%	92-97%
EDTA plasma(n=5)	90-95%	94-106%	105-107%	87-98%
heparin plasma(n=5)	95-103%	91-99%	104-107%	98-105%

Method Type

Sandwich

Platform

PF00340

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

Protein Name

Interleukin-18

Recovery

Recovery was determined by spiking various levels of IL-18 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	102	96-106
Plasma	101	99-104

Sensitivity

2.9pg/mL

Sequence

50:

MSCEEIAVCA ; VRLRENLCLY ; FEELECDAFC ; KDKTIKRFFR ; NVNSQLLVVR ;

100:

PDLNVAAFED ; VTDQEVKSGS ; GMYFDIHCYK ; TTAPSARMPV ; AFSVQVEDKS ;

150:

YYMCCEKEHG ; KMVVRFREGE ; VPKDIPGESN ; IIFFKKTFTS ; CSSKAFKFEY ;

196:

SLEQGMFLAF ; EEEDSLRKLI ; LKKLPREDEV ; DETTKFVTSH ; NERHNL

Sequence Length

196

String

Q8QFQ8

Uniprot	Q8QFQ8
Gene Name	IL18
Immunogen Species	Gallus Gallus (Chicken)
Unigene	Gga.42408

Reference	1. "Cloning and characterisation of chicken interleukin-18." Rothwell L., Buerstedde J.-M., Kaiser P. Submitted (2000-03) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]

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SKU	Supplier No.	Size	Your price	Quantity
BHE13806232	E0064c	96T	$900		Add to Cart

ELISA Kit for Chicken Interleukin-18

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