ELISA Kit for Chicken Interferon gamma

Mfr.No.

E0049c

Categories

Elisa

Function

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Specificity

Natural and recombinant Chicken Interferon gamma

Subcellular Location

Secreted

Detection Range

15.6-1000pg/mL

Entryname

IFNG_CHICK

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFNG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-104%	96-100%	92-98%	102-105%
EDTA plasma(n=5)	89-95%	93-105%	98-104%	93-97%
heparin plasma(n=5)	96-103%	97-101%	99-105%	91-98%

Method Type

Sandwich

Platform

PF00714

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Protein Name

Interferon gamma

Recovery

Recovery was determined by spiking various levels of IFNG into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	98	96-100
Plasma	99	97-101

Sensitivity

5.3pg/mL

Sequence

50:

MTCQTYNLFV ; LSVIMIYYGH ; TASSLNLVQL ; QDDIDKLKAD ; FNSSHSDVAD ;

100:

GGPIIVEKLK ; NWTERNEKRI ; ILSQIVSMYL ; EMLENTDKSK ; PHIKHISEEL ;

150:

YTLKNNLPDG ; VKKVKDIMDL ; AKLPMNDLRI ; QRKAANELFS ; ILQKLVDPPS ;

164:

FKRKRSQSQR ; RCNC

Sequence Length

164

String

P49708

Sub Unit

Homodimer.

Uniprot	P49708
Gene Name	IFNG
Immunogen Species	Gallus Gallus (Chicken)
Kegg	gga:396054
Unigene	Gga.47882

Reference	1. "Cloning and expression of the chicken interferon-gamma gene." Digby M.R., Lowenthal J.W. J. Interferon Cytokine Res.15:939-945(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. "Biological properties of recombinant chicken interferon-gamma." Weining K.C., Schultz U., Muenster U., Kaspers B., Staeheli P. Eur. J. Immunol.26:2440-2447(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 3. "Structure of the chicken interferon-gamma gene, and comparison to mammalian homologues." Kaiser P., Wain H.M., Rothwell L. Gene207:25-32(1998) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] strain: N LINE. 4. "Molecular genetic analysis of chicken interferon gamma gene." Song K.D., Kim D.K., Choi K.D., Lillehoj H.S., Han I.K., Han J.Y. Submitted (1997-04) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] strain: White leghorn. tissue: Spleen. 5. "Chicken interferon-gamma." Lv Y.Z., Cao Y.C., Bi Y.Z. Submitted (2001-09) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Spleen. 6. "Cloning and expression of the chicken interferon-gamma gene." Bao M., Zhong D., Xu F., Yu W. Submitted (2003-12) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Spleen. 7. "Cloning and sequence analysis of interferon gamma of China Lushi chicken." Zhang X., Zhang C., Li Y., Cheng X., Wu T. Submitted (2009-02) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Peripheral blood lymphocyte.

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ELISA Kit for Chicken Interferon gamma

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