ELISA Kit for Chicken Angiotensin-converting enzyme

Mfr.No.

E0004c

Alternative Names

Dipeptidyl carboxypeptidase I ; Kininase II ; DCP1

Categories

Elisa

Function

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin.

Specificity

Natural and recombinant Chicken Angiotensin-converting enzyme

Subcellular Location

Membrane Single-pass type I membrane protein

Detection Range

0.312-20ng/mL

Entryname

ACE_CHICK

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
Saliva(n=3)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF01401

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

Angiotensin-converting enzyme

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sensitivity

0.15ng/mL

Sequence

50:

AKELYGNIWS ; NFSDPQLKKI ; IGSIQTLGPS ; NLPLDKRQQY ; NTILSDMDKI ;

100:

YSTAKVCLDN ; GTCWDLEPDI ; SDIMATSRSY ; KKLLYAWEGW ; HNAAGNPLRA ;

150:

KYQEFVTLSN ; EAYQMDGFED ; TGSYWRSWYD ; STTFEDDLEH ; LYNQLEPLYL ;

200:

NLHAFVRRKL ; YDRYGPKYIN ; LKGPIPAHLL ; GNMWAQQWNN ; IYDLMVPYPD ;

250:

KPNLDVTNTM ; VNQGWNATHM ; FRVSEEFFTS ; LGLLEMPPEF ; WEKSMLEKPA ;

300:

DGREVVCHAS ; AWDFYNRKDF ; RIKQCTTVTM ; EQLFTVHHEM ; GHVQYYLQYK ;

350:

DQPVSFRGGA ; NPGFHEAIGD ; VLSLSVSTPS ; HLQKIGLLSS ; AVEDEESNIN ;

400:

YLLKMALEKI ; AFLPFGYLID ; QWRWNVFSGR ; TPPSRYNYDW ; WYLRTKYQGI ;

450:

CAPVSRNESN ; FDPGAKYHIP ; GNTPYIRYFV ; SFILQFQFHK ; ALCQAANHTG ;

500:

PLHTCDIYMS ; KEAGAKLREV ; LKAGSSKSWQ ; EILFNLTGTD ; KMDAGALLEY ;

550:

FSPVTTWLQE ; QNNKTNEVLG ; WPEFDWRSPI ; PEGYPEGIDK ; IVDEAQAKEF ;

600:

LSEYNSTAEV ; VWNAYTEASW ; EYNTNITDHN ; KEVMLEKNLA ; MSKHTIEYGM ;

650:

RARQFDPSDF ; QDETVTRILN ; KLSVLERAAL ; PEDELKEYNT ; LLSDMETTYS ;

700:

VAKVCRENNT ; FHPLDPDLTD ; ILATSRDYNE ; LLFAWKGWWD ; ASGAKIKDKY ;

750:

KRYVELSNKA ; AVLNGYTDNG ; AYWRSLYETP ; TFEEDLERLY ; LQLQPLYLNL ;

800:

HAYVRRALYN ; KYGAEHISLK ; GPIPAHLLGN ; MWAQSWSNIF ; DLVMPFPDAT ;

850:

KVDATPAMKQ ; QGWTPKMMFE ; ESDRFFTSLG ; LIPMPQEFWD ; KSMIEKPADG ;

900:

REVVCHASAW ; DFYNRKDFRI ; KQCTVVNMDD ; LITVHHEMGH ; VQYFLQYMDQ ;

950:

PISFRDGANP ; GFHEAIGDVM ; ALSVSTPKHL ; HSINLLDQVT ; ENEESDINYL ;

1000:

MSIALDKIAF ; LPFGYLMDQW ; RWKVFDGRIK ; EDEYNQQWWN ; LRLKYQGLCP ;

1050:

PVPRSEDDFD ; PGAKFHIPAN ; VPYIRYFVSF ; VIQFQFHQAL ; CKAAGHTGPL ;

1100:

HTCDIYQSKE ; AGKLLGDAMK ; LGFSKPWPEA ; MQLITGQPNM ; SAEALMSYFE ;

1150:

PLMTWLVKKN ; TENGEVLGWP ; EYSWTPYAVT ; EFHAATDTAD ; FLGMSVGTKQ ;

1193:

ATAGAWVLLA ; LALVFLITSI ; FLGVKLFSSR ; RKAFKSSSEM ; ELK

Sequence Length

1193

String

Q10751

Uniprot	Q10751
Gene Name	ACE
Immunogen Species	Gallus Gallus (Chicken)
Unigene	Gga.3781

Reference	1. "Chicken lacks the testis specific isozyme of angiotensin converting enzyme found in mammals." Esther C.R., Thomas K.E., Bernstein K.E. Biochem. Biophys. Res. Commun.205:1916-1921(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Lung.

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ELISA Kit for Chicken Angiotensin-converting enzyme

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