ELISA Kit for Chicken Angiogenin

Mfr.No.

E0007c

Alternative Names

Ribonuclease A

Categories

Elisa

Function

May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.

Specificity

Natural and recombinant Chicken Angiogenin

Subcellular Location

Secreted

Detection Range

78-5000pg/mL

Entryname

ANGI_CHICK

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-99%	91-102%	93-103%	90-104%
EDTA plasma(n=5)	89-98%	90-103%	91-97%	92-102%
heparin plasma(n=5)	90-101%	92-105%	94-107%	91-106%

Method Type

Sandwich

Platform

PF00074

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 5.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 9.2%

Protein Name

Angiogenin

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	95	91-101
Plasma	94	90-99

Sensitivity

39pg/mL

Sequence

50:

MAMSSLWWTA ; ILLLALTVSM ; CYGVPTYQDF ; LRTHVDFPKT ; SFPNIAAYCN ;

100:

VMMVRRGINV ; HGRCKSLNTF ; VHTDPRNLNT ; LCINQPNRAL ; RTTQQQLPVT ;

139:

DCKLIRSHPT ; CSYTGNQFNH ; RVRVGCWGGL ; PVHLDGTFP

Sequence Length

139

Sub Unit

Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur.

Uniprot	P27043
Gene Name	ANG
Immunogen Species	Gallus Gallus (Chicken)
Kegg	gga:396194
Unigene	Gga.703

Reference	1. "Identification of genes differentially expressed in two types of v-myb-transformed avian myelomonocytic cells." Nakano T., Graf T. Oncogene7:527-534(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] strain: White leghorn. tissue: Bone marrow.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13800740	E0007c	96T	$900		Add to Cart

ELISA Kit for Chicken Angiogenin

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