ELISA Kit for Chicken Adenosine receptor A1

Mfr.No.

E12273c

Categories

Elisa

Function

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.

Specificity

Natural and recombinant Chicken Adenosine receptor A1

Subcellular Location

Cell membrane Multi-pass membrane protein

Entryname

AA1R_CHICK

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AA1R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	94-108%	97-106%	95-106%	92-110%
EDTA plasma(n=5)	92-107%	95-105%	91-108%	94-105%
heparin plasma(n=5)	95-103%	93-108%	93-107%	90-110%

Method Type

Sandwich

Platform

PF00001

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.2%

Protein Name

Adenosine receptor A1

Recovery

Recovery was determined by spiking various levels of AA1R into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	93-104
Plasma	100	92-106

Sequence

50:

MAQSVTAFQA ; AYISIEVLIA ; LVSVPGNILV ; IWAVKMNQAL ; RDATFCFIVS ;

100:

LAVADVAVGA ; LVIPLAIIIN ; IGPQTEFYSC ; LMMACPVLIL ; TESSILALLA ;

150:

IAVDRYLRVK ; IPVRYKSVVT ; PRRAAVAIAC ; CWIVSFLVGL ; TPMFGWNNLN ;

200:

KVLGTRDLNV ; SHSEFVIKCQ ; FETVISMEYM ; VYFNFFVWVL ; PPLLLMLLIY ;

250:

LEVFNLIRTQ ; LNKKVSSSSN ; DPQKYYGKEL ; KIAKSLALVL ; FLFALSWLPL ;

300:

HILNCITLFC ; PSCKTPHILT ; YIAIFLTHGN ; SAMNPIVYAF ; RIKKFRTAFL ;

324:

QIWNQYFCCK ; TNKSSSSSTA ; ETVN

Sequence Length

324

Uniprot	P49892
Gene Name	ADORA1
Immunogen Species	Gallus Gallus (Chicken)
Kegg	gga:374212
Unigene	Gga.4980

Reference	1. "Isolation and characterization of an avian A1 adenosine receptor gene and a related cDNA clone." Aguilar J.S., Tan F., Durand I., Green R.D. Biochem. J.307:729-734(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] tissue: Adipose tissue.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13800307	E12273c	96T	$900		Add to Cart

ELISA Kit for Chicken Adenosine receptor A1

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