ELISA Kit for Chicken 14-3-3 protein theta

Mfr.No.

E14293c

Alternative Names

RCJMB04_2i3

Categories

Elisa

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Specificity

Natural and recombinant Chicken 14-3-3 protein theta

Subcellular Location

Cytoplasm

Entryname

1433T_CHICK

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 14-3-3 protein theta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-106%	95-102%	90-110%	96-104%
EDTA plasma(n=5)	91-104%	93-103%	92-106%	93-103%
heparin plasma(n=5)	94-101%	91-107%	96-101%	92-105%

Method Type

Sandwich

Platform

PF00244

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.5%

Protein Name

14-3-3 protein theta

Recovery

Recovery was determined by spiking various levels of 14-3-3 protein theta into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	94-103
Plasma	98	92-101

Sequence

50:

MDKTELIQKA ; KLAEQAERYD ; DMATCMKAVT ; EQGAELSNEE ; RNLLSVAYKN ;

100:

VVGGRRSAWR ; VISSIEQKTD ; TSDKKMQLIK ; DYREKVESEL ; RSICTTVLEL ;

150:

LDKYLIANAT ; NPESKVFYLK ; MKGDYFRYLA ; EVACGDDRKQ ; TIENSQGAYQ ;

200:

EAFDISKKEM ; QPTHPIRLGL ; ALNFSVFYYE ; ILNNPELACT ; LAKTAFDEAI ;

245:

AELDTLNEDS ; YKDSTLIMQL ; LRDNLTLWTS ; DSAGEECDAA ; EGAEN

Sequence Length

245

String

Q5ZMD1

Sub Unit

Homodimer, and heterodimer with other family members.

Uniprot	Q5ZMD1
Gene Name	YWHAQ
Immunogen Species	Gallus Gallus (Chicken)
Kegg	gga:421932
Unigene	Gga.8471

Reference	1. "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol.6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: CB. tissue: Bursa of Fabricius.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13800184	E14293c	96T	$900		Add to Cart

ELISA Kit for Chicken 14-3-3 protein theta

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