ELISA Kit for Bovine Thyroglobulin

Mfr.No.

E0355b

Categories

Elisa

Function

Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).

Specificity

Natural and recombinant Bovine Thyroglobulin

Subcellular Location

Secreted

Detection Range

0.312-20ng/mL

Entryname

THYG_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
Saliva(n=3)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF00135

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

Thyroglobulin

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sensitivity

0.022ng/ml

Sequence

50:

MALALWVFGL ; LDLICLASAN ; IFEYQVDAQP ; LRPCELQRER ; AFLKREDYVP ;

100:

QCAEDGSFQT ; VQCGKDGASC ; WCVDADGREV ; PGSRQPGRPA ; ACLSFCQLQK ;

150:

QQILLSSYIN ; STATSYLPQC ; QDSGDYSPVQ ; CDLRRRQCWC ; VDAEGMEVYG ;

200:

TRQQGRPARC ; PRSCEIRNRR ; LLHGVGDRSP ; PQCSPDGAFR ; PVQCKLVNTT ;

250:

DMMIFDLVHS ; YSRFPDAFVT ; FSSFRSRFPE ; VSGYCYCADS ; QGRELAETGL ;

300:

ELLLDEIYDT ; IFAGLDLAST ; FAETTLYRIL ; QRRFLAVQLV ; ISGRFRCPTK ;

350:

CEVERFAATS ; FRHPYVPSCH ; PDGEYQAAQC ; QQGGPCWCVD ; SRGQEIPGTR ;

400:

QRGEPPSCAE ; DQSCPSERRR ; AFSRLRFGPS ; GYFSRRSLLL ; APEEGPVSQR ;

450:

FARFTASCPP ; SIKELFLDSG ; IFQPMLQGRD ; TRFVAPESLK ; EAIRGLFPSR ;

500:

ELARLALQFT ; TNAKRLQQNL ; FGGRFLVKVG ; QFNLSGALGT ; RGTFNFSHFF ;

550:

QQLGLPGFQD ; GRALADLAKP ; LSVGLNSNPA ; SEAPKASKID ; VALRKPVVGS ;

600:

FGFEVNLQEN ; QNALQFLSSF ; LELPEFLLFL ; QHAISVPEDI ; ARDLGDVMEM ;

650:

VFSSQGCGQA ; PGSLFVPACT ; AEGSYEEVQC ; FAGDCWCVDA ; QGRELAGSRV ;

700:

RGGRPRCPTE ; CEKQRARMQS ; LLGSQPAGSS ; LFVPACTSKG ; NFLPVQCFNS ;

750:

ECYCVDTEGQ ; PIPGTRSALG ; EPKKCPSPCQ ; LQAERAFLGT ; VRTLVSNPST ;

800:

LPALSSIYIP ; QCSASGQWSP ; VQCDGPPEQA ; FEWYERWEAQ ; NSAGQALTPA ;

850:

ELLMKIMSYR ; EAASRNFRLF ; IQNLYEAGQQ ; GIFPGLARYS ; SFQDVPVSVL ;

900:

EGNQTQPGGN ; VFLEPYLFWQ ; ILNGQLDRYP ; GPYSDFSAPL ; AHFDLRSCWC ;

950:

VDEAGQKLEG ; TRNEPNKVPA ; CPGSCEEVKL ; RVLQFIREAE ; EIVTYSNSSR ;

1000:

FPLGESFLAA ; KGIRLTDEEL ; AFPPLSPSRE ; TFLEKFLSGS ; DYAIRLAAQS ;

1050:

TFDFYQRRLV ; TLAESPRAPS ; PVWSSAYLPQ ; CDAFGGWEPV ; QCHAATGHCW ;

1100:

CVDGKGEYVP ; TSLTARSRQI ; PQCPTSCERL ; RASGLLSSWK ; QAGVQAEPSP ;

1150:

KDLFIPTCLE ; TGEFARLQAS ; EAGTWCVDPA ; SGEGVPPGTN ; SSAQCPSLCE ;

1200:

VLQSGVPSRR ; TSPGYSPACR ; AEDGGFSPVQ ; CDPAQGSCWC ; VLGSGEEVPG ;

1250:

TRVAGSQPAC ; ESPQCPLPFS ; VADVAGGAIL ; CERASGLGAA ; AGQRCQLRCS ;

1300:

QGYRSAFPPE ; PLLCSVQRRR ; WESRPPQPRA ; CQRPQFWQTL ; QTQAQFQLLL ;

1350:

PLGKVCSADY ; SGLLLAFQVF ; LLDELTARGF ; CQIQVKTAGT ; PVSIPVCDDS ;

1400:

SVKVECLSRE ; RLGVNITWKL ; QLVDAPPASL ; PDLQDVEEAL ; AGKYLAGRFA ;

1450:

DLIQSGTFQL ; HLDSKTFSAD ; TSIRFLQGDR ; FGTSPRTQFG ; CLEGFGRVVA ;

1500:

ASDASQDALG ; CVKCPEGSYF ; QDEQCIPCPA ; GFYQEQAGSL ; ACVPCPEGRT ;

1550:

TVYAGAFSQT ; HCVTDCQKNE ; VGLQCDQDSQ ; YRASQRDRTS ; GKAFCVDGEG ;

1600:

RRLPWTEAEA ; PLVDAQCLVM ; RKFEKLPESK ; VIFSADVAVM ; VRSEVPGSES ;

1650:

SLMQCLADCA ; LDEACGFLTV ; STAGSEVSCD ; FYAWASDSIA ; CTTSGRSEDA ;

1700:

LGTSQATSFG ; SLQCQVKVRS ; REGDPLAVYL ; KKGQEFTITG ; QKRFEQTGFQ ;

1750:

SALSGMYSPV ; TFSASGASLA ; EVHLFCLLAC ; DHDSCCDGFI ; LVQVQGGPLL ;

1800:

CGLLSSPDVL ; LCHVRDWRDP ; AEAQANASCP ; GVTYDQDSRQ ; VTLRLGGQEI ;

1850:

RGLTPLEGTQ ; DTLTSFQQVY ; LWKDSDMGSR ; SESMGCRRDT ; EPRPASPSET ;

1900:

DLTTGLFSPV ; DLIQVIVDGN ; VSLPSQQHWL ; FKHLFSLQQA ; NLWCLSRCAG ;

1950:

EPSFCQLAEV ; TDSEPLYFTC ; TLYPEAQVCD ; DILESSPKGC ; RLILPRRPSA ;

2000:

LYRKKVVLQD ; RVKNFYNRLP ; FQKLTGISIR ; NKVPMSDKSI ; SSGFFECERL ;

2050:

CDMDPCCTGF ; GFLNVSQLKG ; GEVTCLTLNS ; LGLQTCSEEY ; GGVWRILDCG ;

2100:

SPDTEVRTYP ; FGWYQKPVSP ; SDAPSFCPSV ; ALPALTENVA ; LDSWQSLALS ;

2150:

SVIVDPSIRN ; FDVAHISTAA ; VGNFSAARDR ; CLWECSRHQD ; CLVTTLQTQP ;

2200:

GAVRCMFYAD ; TQSCTHSLQA ; QNCRLLLHEE ; ATYIYRKPNI ; PLPGFGTSSP ;

2250:

SVPIATHGQL ; LGRSQAIQVG ; TSWKPVDQFL ; GVPYAAPPLG ; EKRFRAPEHL ;

2300:

NWTGSWEATK ; PRARCWQPGI ; RTPTPPGVSE ; DCLYLNVFVP ; QNMAPNASVL ;

2350:

VFFHNAAEGK ; GSGDRPAVDG ; SFLAAVGNLI ; VVTASYRTGI ; FGFLSSGSSE ;

2400:

LSGNWGLLDQ ; VVALTWVQTH ; IQAFGGDPRR ; VTLAADRGGA ; DIASIHLVTT ;

2450:

RAANSRLFRR ; AVLMGGSALS ; PAAVIRPERA ; RQQAAALAKE ; VGCPSSSVQE ;

2500:

MVSCLRQEPA ; RILNDAQTKL ; LAVSGPFHYW ; GPVVDGQYLR ; ETPARVLQRA ;

2550:

PRVKVDLLIG ; SSQDDGLINR ; AKAVKQFEES ; QGRTSSKTAF ; YQALQNSLGG ;

2600:

EAADAGVQAA ; ATWYYSLEHD ; SDDYASFSRA ; LEQATRDYFI ; ICPVIDMASH ;

2650:

WARTVRGNVF ; MYHAPESYSH ; SSLELLTDVL ; YAFGLPFYPA ; YEGQFTLEEK ;

2700:

SLSLKIMQYF ; SNFIRSGNPN ; YPHEFSRRAP ; EFAAPWPDFV ; PRDGAESYKE ;

2750:

LSVLLPNRQG ; LKKADCSFWS ; KYIQSLKASA ; DETKDGPSAD ; SEEEDQPAGS ;

2769:

GLTEDLLGLP ; ELASKTYSK

Sequence Length

2769

String

P01267

Sub Unit

Homodimer.

Uniprot	P01267
Gene Name	TG
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:280706
Unigene	Bt.88019

Reference	1. "Primary structure of bovine thyroglobulin deduced from the sequence of its 8,431-base complementary DNA." Mercken L., Simons M.-J., Swillens S., Massaer M., Vassart G. Nature316:647-651(1985) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. "Presence of hormonogenic and repetitive domains in the first 930 amino acids of bovine thyroglobulin as deduced from the cDNA sequence." Mercken L., Simons M.-J., de Martynhoff G., Swillens S., Vassart G. Eur. J. Biochem.147:59-64(1985) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-930 3. "Structural organization of the bovine thyroglobulin gene and of its 5'-flanking region." de Martynhoff G., Pohl V., Mercken L., van Ommen G.-J., Vassart G. Eur. J. Biochem.164:591-599(1987) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22 4. "Structural organization of the 5' region of the thyroglobulin gene. Evidence for intron loss and 'exonization' during evolution." Parma J., Christophe D., Pohl V., Vassart G. J. Mol. Biol.196:769-779(1987) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1002-1209 5. "Thyroglobulin glycosylation: location and nature of the N-linked oligosaccharide units in bovine thyroglobulin." Rawitch A.B., Pollock H.G., Yang S.X. Arch. Biochem. Biophys.300:271-279(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-110; ASN-483; ASN-495; ASN-853; ASN-947; ASN-1140; ASN-1365; ASN-1776; ASN-1870; ASN-2014; ASN-2123; ASN-2251 AND ASN-2296;ABSENCE OF GLYCOSYLATION AT ASN-198;STRUCTURE OF CARBOHYDRATE

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ELISA Kit for Bovine Thyroglobulin

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