ELISA Kit for Bovine Regulator of G-protein signaling 19

Mfr.No.

E5248b

Categories

Elisa

Function

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, with the order G(i)a3 > G(i)a1 > G(o)a >> G(z)a/G(i)a2. Activity on G(z)-alpha is inhibited by phosphorylation and palmitoylation of the G-protein.

Specificity

Natural and recombinant Bovine Regulator of G-protein signaling 19

Subcellular Location

Membrane Lipid-anchor

Entryname

RGS19_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RGS19 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	86-100%	84-101%	89-95%	87-97%
EDTA plasma(n=5)	88-99%	90-97%	85-102%	84-105%
heparin plasma(n=5)	85-105%	91-98%	91-96%	89-99%

Platform

PF00615

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.4%

Protein Name

Regulator of G-protein signaling 19

Recovery

Recovery was determined by spiking various levels of RGS19 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	92	85-97
Plasma	94	88-100

Sequence

50:

MPTPPEAEKQ ; QTGPEEADQP ; PSMSSHDAAP ; PAPPRRNPCC ; LCWCCCCSCS ;

100:

WNEERRRAWR ; ASRESRLQPL ; PSCEVCATPT ; PTPTPTPEEV ; RSWAQSFDKL ;

150:

MHSPAGRSVF ; REFLRTEYSE ; ENMLFWLACE ; ELKAEANQHV ; VDEKARLIYE ;

200:

DYVSILSPKE ; VSLDSRVREG ; INKKMQEPSA ; HTFDDAQLQI ; YTLMHRDSYP ;

223:

RFLSSPAYRA ; LLLQGASQSS ; SEA

Sequence Length

223

String

Q08DC7

Sub Unit

Interacts with GIPC PDZ domain.

Uniprot	Q08DC7
Gene Name	RGS19
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:539036
Unigene	Bt.20549

Reference	1. NIH - Mammalian Gene Collection (MGC) project Submitted (2006-09) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: Hereford. tissue: Thalamus.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13809314	E5248b	96T	$900		Add to Cart

ELISA Kit for Bovine Regulator of G-protein signaling 19

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